In JoVE (1)
Other Publications (1)
Articles by Binil Raj in JoVE
Ascending Aortic Constriction in Rats for Creation of Pressure Overload Cardiac Hypertrophy Model Ajith Kumar GS*1, Binil Raj*1, Santhosh Kumar S2, Sanjay G3, Chandrasekharan Cheranellore Kartha1 1Cardio Vascular Disease Biology, Rajiv Gandhi Centre for Biotechnology, 2Animal Research Facility, Rajiv Gandhi Centre for Biotechnology, 3Cardiology Department, Sree Chitra Tirunal Institute for Medical Sciences & Technology We describe a stepwise procedure for creating pressure overload and left ventricular hypertrophy in Wistar rats by constriction of the ascending aorta using a small metallic clip. This model is extensively used for studying remodeling changes during cardiac hypertrophy and for identifying and evaluating strategies for regression of such changes.
Other articles by Binil Raj on PubMed
In vitro Antioxidant Activities of the Methanol Extract and Its Different Solvent Fractions Obtained from the Fruit Pericarp of Terminalia Bellerica Natural Product Research. Feb, 2011 | Pubmed ID: 21294040 Terminalia bellerica has been used as a traditional medicine in a variety of ailments including anaemia, asthma, cancer, inflammation, rheumatism and hypertension. In this study, the free radical scavenging and antioxidant activities of methanol extract (ME) and its different solvent fractions (namely hexane (HE), ethyl acetate (EA), butanol (BL) and water (WA)) of the T. bellerica fruit pericarp were evaluated and compared with standard antioxidant compounds like gallic acid (GA), catechin and ascorbic acid. Among the different fractions tested, the EA fraction exhibited higher antioxidant and radical scavenging activity against 1,1-diphenyl-2-picrylhydrazyl (DPPH), superoxide and hydroxyl radicals than the other fractions, which may be attributed to its higher phenolic and flavonoid content, since a linear relation was observed between the phenolic content and the antioxidant parameters. The HPTLC analysis of the EA fraction revealed that it mainly contains GA and ferulic acid (FA) as major phenolics, and the higher antioxidant activities of EA fraction may be due to the presence of these compounds.