In JoVE (1)
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Articles by Blair Hardman in JoVE
Generation and Identification of GM-CSF Derived Alveolar-like Macrophages and Dendritic Cells From Mouse Bone Marrow Yifei Dong1, Arif A. Arif1, Grace F. T. Poon1, Blair Hardman1, Manisha Dosanjh1, Pauline Johnson1 1Microbiology and Immunology, University of British Columbia Bone marrow cells cultured with granulocyte macrophage colony stimulating factor (GM-CSF) generate a heterogeneous culture containing macrophages and dendritic cells (DCs). This method highlights using MHCII and hyaluronan (HA) binding to differentiate macrophages from the DCs in the GM-CSF culture. Macrophages in this culture have many similarities to alveolar macrophages.