In JoVE (1)
Other Publications (1)
Articles by Brian R. Lee in JoVE
Preparation of Acute Brain Slices Using an Optimized N-Methyl-D-glucamine Protective Recovery Method Jonathan T. Ting*1, Brian R. Lee*1, Peter Chong1, Gilberto Soler-Llavina1, Charles Cobbs2, Christof Koch1, Hongkui Zeng1, Ed Lein1 1Cell Types Program, Allen Institute for Brain Science, 2The Ben and Catherine Ivy Center for Advanced Brain Tumor Treatment, Swedish Neuroscience Institute This protocol demonstrates the implementation of an optimized N-methyl-D-glucamine (NMDG) protective recovery method of brain slice preparation. A single media formulation is used to reliably obtain healthy brain slices from animals of any age and for diverse experimental applications.
Other articles by Brian R. Lee on PubMed
Bidirectional Modulation of Incubation of Cocaine Craving by Silent Synapse-based Remodeling of Prefrontal Cortex to Accumbens Projections Neuron. Sep, 2014 | Pubmed ID: 25199705 Glutamatergic projections from the medial prefrontal cortex (mPFC) to nucleus accumbens (NAc) contribute to cocaine relapse. Here we show that silent synapse-based remodeling of the two major mPFC-to-NAc projections differentially regulated the progressive increase in cue-induced cocaine seeking after withdrawal (incubation of cocaine craving). Specifically, cocaine self-administration in rats generated AMPA receptor-silent glutamatergic synapses within both infralimbic (IL) and prelimbic mPFC (PrL) to NAc projections, measured after 1 day of withdrawal. After 45 days of withdrawal, IL-to-NAc silent synapses became unsilenced/matured by recruiting calcium-permeable (CP) AMPARs, whereas PrL-to-NAc silent synapses matured by recruiting non-CP-AMPARs, resulting in differential remodeling of these projections. Optogenetic reversal of silent synapse-based remodeling of IL-to-NAc and PrL-to-NAc projections potentiated and inhibited, respectively, incubation of cocaine craving on withdrawal day 45. Thus, pro- and antirelapse circuitry remodeling is induced in parallel after cocaine self-administration. These results may provide substrates for utilizing endogenous antirelapse mechanisms to reduce cocaine relapse.