In JoVE (1)
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Articles by Cassi Estrem in JoVE
High-resolution Imaging and Analysis of Individual Astral Microtubule Dynamics in Budding Yeast Colby P. Fees1, Cassi Estrem1, Jeffrey K. Moore1 1Department of Cell and Developmental Biology, University of Colorado School of Medicine Budding yeast is an advantageous model for studying microtubule dynamics in vivo due to its powerful genetics and the simplicity of its microtubule cytoskeleton. The following protocol describes how to transform and culture yeast cells, acquire confocal microscopy images, and quantitatively analyze microtubule dynamics in living yeast cells.