Other Publications (1)
Articles by Cheng Yong Tham in JoVE
Live-imaging of Breast Epithelial Cell Migration After the Transient Depletion of TIP60 Yanzhou Zhang1, Grace SuShin Chia1, Cheng Yong Tham1, Sudhakar Jha1,2 1Cancer Science Institute of Singapore, National University of Singapore, 2Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore Here, we present the real-time monitoring of cell migration in a wound-healing assay using TIP60-depleted MCF10A breast epithelial cells. The implementation of live-cell imaging techniques in our protocol allows us to analyze and visualize single-cell movement in real time and across time.
Other articles by Cheng Yong Tham on PubMed
TIP60 Inhibits Metastasis by Ablating DNMT1-SNAIL2-driven Epithelial-mesenchymal Transition Program Journal of Molecular Cell Biology. | Pubmed ID: 27651430 HIV-Tat-interacting protein of 60 kDa (TIP60) is a lysine acetyltransferase and known to be downregulated in multiple cancers. Among various signalling pathways, TIP60 is implicated in regulating epithelial-mesenchymal transition (EMT). Here, we show that TIP60 expression abrogates cell migration and metastatic potential of breast cancer cells using in vitro and in vivo models. Mechanistically, we show that this is through its ability to destabilize DNMT1 and inhibit SNAIL2 function (SNAIL2-mediated EMT/cell migration). Depletion of TIP60 stabilizes DNMT1 and increases SNAIL2 levels, resulting in EMT. Recruitment of DNMT1 to the SNAIL2 targets in the absence of TIP60 increases DNA methylation on their promoter region and further represses the expression of epithelial markers. In pathophysiological scenario, we find TIP60 to be significantly downregulated in breast cancer patients with poor overall survival and disease-free survival prognoses. These data suggest that levels of TIP60 can be a prognostic marker of breast cancer progression and stabilization of TIP60 could be a promising strategy to treat cancers.