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Articles by Chin-Hsien Tsai in JoVE
Analyses of Mitochondrial Calcium Influx in Isolated Mitochondria and Cultured Cells Joshua T. Maxwell1, Chin-Hsien Tsai1, Tahmina A. Mohiuddin2, Jennifer Q. Kwong1 1Department of Pediatrics, Emory University School of Medicine, 2Emory College of Arts and Sciences, Emory University Here, we present two protocols for the measurement of mitochondrial Ca2+ influx in isolated mitochondria and cultured cells. For isolated mitochondria, we detail a plate reader-based Ca2+ import assay using the Ca2+ sensitive dye calcium green-5N. For cultured cells, we describe a confocal microscopy method using the Ca2+ dye Rhod-2/AM.