Articles by Christian Njoo in JoVE
Other articles by Christian Njoo on PubMed
造血集落刺激因子肿瘤神经交互进行调解和骨癌症疼痛。 Nature Medicine. Jul, 2009 | Pubmed ID: 19525966 疼痛是癌症的几种形式与关联最严重和使人虚弱的症状之一。各种类型的癌和肉瘤移转到骨骼骨骼及原因自发骨疼痛和痛觉过敏，这是伴随着骨退化和外周神经重塑。尽管最近取得的进展，开发和维护的癌症诱发疼痛的基本分子机制还不太清楚。几种类型的非造血系统肿瘤分泌作用于髓系细胞和肿瘤细胞的造血集落刺激因子。在这里我们报告对感觉神经功能也表示受体和粒细胞集落-和粒细胞-巨噬细胞集落刺激因子 (G-CSF、 GM-CSF） 信号调解员。GM-CSF 敏感神经对机械刺激体外和体内，强化钙素释放和造成皮肤感觉神经末梢的萌芽。中断 G-CSF 与 GM-CSF 信号体内导致减少的肿瘤的生长和神经重塑，和废除骨癌症疼痛。GM-CSF 感觉神经元信号转导的关键意义被揭示的衰减后感官神经特定组合式 GM-CSF 受体的肿瘤诱发疼痛。这些结果表明 G-CSF 和 GM-CSF 非常重要在肿瘤神经交互，表明其受体在原发性神经传入纤维构成潜在的治疗目标，在癌痛。
A Novel Biological Role for the Phospholipid Lysophosphatidylinositol in Nociceptive Sensitization Via Activation of Diverse G-protein Signalling Pathways in Sensory Nerves in Vivo Pain. Dec, 2013 | Pubmed ID: 23973358 The rich diversity of lipids and the specific signalling pathways they recruit provides tremendous scope for modulation of biological functions. Lysophosphatidylinositol (LPI) is emerging as a key modulator of cell proliferation, migration, and function, and holds important pathophysiological implications due to its high levels in diseased tissues, such as in cancer. Here we report a novel role for LPI in sensitization of peripheral sensory neurons, which was evident as exaggerated sensitivity to painful and innocuous pressure. Histopathological analyses indicated lack of involvement of myelin pathology and immune cell recruitment by LPI. Using pharmacological and conditional genetic tools in mice, we delineated receptor-mediated from non-receptor-mediated effects of LPI and we observed that GPR55, which functions as an LPI receptor when heterologously expressed in mammalian cells, only partially mediates LPI-induced actions in the context of pain sensitization in vivo; we demonstrate that, in vivo, LPI functions by activating Gα(13) as well as Gα(q/11) arms of G-protein signalling in sensory neurons. This study thus reports a novel pathophysiological function for LPI and elucidates underlying molecular mechanisms.