In JoVE (1)
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Articles by Christopher W. Doucette in JoVE
Application of Stopped-flow Kinetics Methods to Investigate the Mechanism of Action of a DNA Repair Protein F. Noah Biro1, Jie Zhai1, Christopher W. Doucette1, Manju M. Hingorani1 1Molecular Biology and Biochemistry Department, Wesleyan University Msh2-Msh6 is responsible for initiating repair of replication errors in DNA. Here we present a transient kinetics approach towards understanding how this critical protein works. The report illustrates stopped-flow experiments for measuring the coupled DNA binding and ATPase kinetics underlying Msh2-Msh6 mechanism of action in DNA repair.