In JoVE (1)
Articles by Christopher Bartos in JoVE
Safety Precautions and Operating Procedures in an (A)BSL-4 Laboratory: 4. Medical Imaging Procedures Russell Byrum1, Lauren Keith1, Christopher Bartos1, Marisa St. Claire1, Matthew G. Lackemeyer1, Michael R. Holbrook1, Krisztina Janosko1, Jason Barr1, Daniela Pusl1, Laura Bollinger1, Jiro Wada1, Linda Coe1, Lisa E. Hensley1, Peter B. Jahrling1, Jens H. Kuhn1, Margaret R. Lentz1 1Integrated Research Facility at Frederick, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH) Here, we present an overview of the preparation and animal handling procedures required to safely perform medical imaging in an animal biosafety level 4 laboratory. Computed tomography of a mock-infected guinea pig illustrates these procedures that may be used to evaluate the disease caused by a high consequence pathogen.
Other articles by Christopher Bartos on PubMed
Intratracheal Exposure of Common Marmosets to MERS-CoV Jordan-n3/2012 or MERS-CoV EMC/2012 Isolates Does Not Result in Lethal Disease Virology. Nov, 2015 | Pubmed ID: 26342468 Middle East Respiratory Syndrome Coronavirus (MERS-CoV) continues to be a threat to human health in the Middle East. Development of countermeasures is ongoing; however, an animal model that faithfully recapitulates human disease has yet to be defined. A recent study indicated that inoculation of common marmosets resulted in inconsistent lethality. Based on these data we sought to compare two isolates of MERS-CoV. We followed disease progression in common marmosets after intratracheal exposure with: MERS-CoV-EMC/2012, MERS-CoV-Jordan-n3/2012, media, or inactivated virus. Our data suggest that common marmosets developed a mild to moderate non-lethal respiratory disease, which was quantifiable by computed tomography (CT), with limited other clinical signs. Based on CT data, clinical data, and virological data, MERS-CoV inoculation of common marmosets results in mild to moderate clinical signs of disease that are likely due to manipulations of the marmoset rather than as a result of robust viral replication.
3B11-N, a Monoclonal Antibody Against MERS-CoV, Reduces Lung Pathology in Rhesus Monkeys Following Intratracheal Inoculation of MERS-CoV Jordan-n3/2012 Virology. Mar, 2016 | Pubmed ID: 26828465 Middle East Respiratory Syndrome Coronavirus (MERS-CoV) was identified in 2012 as the causative agent of a severe, lethal respiratory disease occurring across several countries in the Middle East. To date there have been over 1600 laboratory confirmed cases of MERS-CoV in 26 countries with a case fatality rate of 36%. Given the endemic region, it is possible that MERS-CoV could spread during the annual Hajj pilgrimage, necessitating countermeasure development. In this report, we describe the clinical and radiographic changes of rhesus monkeys following infection with 5×10(6) PFU MERS-CoV Jordan-n3/2012. Two groups of NHPs were treated with either a human anti-MERS monoclonal antibody 3B11-N or E410-N, an anti-HIV antibody. MERS-CoV Jordan-n3/2012 infection resulted in quantifiable changes by computed tomography, but limited other clinical signs of disease. 3B11-N treated subjects developed significantly reduced lung pathology when compared to infected, untreated subjects, indicating that this antibody may be a suitable MERS-CoV treatment.