Articles by Chunyan Gu-Trantien in JoVE
A Simple and Rapid Protocol to Non-enzymatically Dissociate Fresh Human Tissues for the Analysis of Infiltrating Lymphocytes Soizic Garaud*1,2, Chunyan Gu-Trantien*1,2, Jean-Nicolas Lodewyckx1,2, Anaïs Boisson1,2, Pushpamali De Silva1,2, Laurence Buisseret1,2, Edoardo Migliori1,2, Myriam Libin3, Céline Naveaux1,2, Hugues Duvillier1,4, Karen Willard-Gallo1,2 1Molecular Immunology Unit, Université Libre de Bruxelles, 2Institut Jules Bordet, Université Libre de Bruxelles, 3Institut d'Immunologie Médicale, Université Libre de Bruxelles, 4Flow Cytometry Core Facility, Université Libre de Bruxelles This protocol describes the rapid non-enzymatic dissociation of fresh human tissue fragments for qualitative and quantitative assessment of CD45+ cells (lymphocytes/leukocytes) present in various normal and malignant human tissues. Additionally, the supernatant obtained from the primary tissue homogenate can be collected and stored for further analysis or experimentation.
Other articles by Chunyan Gu-Trantien on PubMed
CD4⁺ Follicular Helper T Cell Infiltration Predicts Breast Cancer Survival The Journal of Clinical Investigation. Jul, 2013 | Pubmed ID: 23778140 CD4⁺ T cells are critical regulators of immune responses, but their functional role in human breast cancer is relatively unknown. The goal of this study was to produce an image of CD4⁺ T cells infiltrating breast tumors using limited ex vivo manipulation to better understand the in vivo differences associated with patient prognosis. We performed comprehensive molecular profiling of infiltrating CD4⁺ T cells isolated from untreated invasive primary tumors and found that the infiltrating T cell subpopulations included follicular helper T (Tfh) cells, which have not previously been found in solid tumors, as well as Th1, Th2, and Th17 effector memory cells and Tregs. T cell signaling pathway alterations included a mixture of activation and suppression characterized by restricted cytokine/chemokine production, which inversely paralleled lymphoid infiltration levels and could be reproduced in activated donor CD4⁺ T cells treated with primary tumor supernatant. A comparison of extensively versus minimally infiltrated tumors showed that CXCL13-producing CD4⁺ Tfh cells distinguish extensive immune infiltrates, principally located in tertiary lymphoid structure germinal centers. An 8-gene Tfh signature, signifying organized antitumor immunity, robustly predicted survival or preoperative response to chemotherapy. Our identification of CD4⁺ Tfh cells in breast cancer suggests that they are an important immune element whose presence in the tumor is a prognostic factor.
Tumor-infiltrating Follicular Helper T Cells: The New Kids on the Block Oncoimmunology. Oct, 2013 | Pubmed ID: 24244900 By analyzing CD4(+) lymphocytes in human breast carcinomas, we have recently uncovered the presence of follicular helper T cells in lesions exhibiting an extensive immune infiltrate. The presence of these specialized CD4(+) T cells, which localize to the germinal centers of peritumoral tertiary lymphoid structures found in extensively infiltrated neoplastic lesions, predicts improved disease outcome among breast carcinoma patients.