In JoVE (1)
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Articles by Crystal R. Noell in JoVE
Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay Heying Cui*1, Kyle M. Loftus*1, Crystal R. Noell1, Sozanne R. Solmaz1 1Department of Chemistry, State University of New York at Binghamton Cyclin-dependent kinase 1 (Cdk1) is activated in the G2 phase of the cell cycle and regulates many cellular pathways. Here, we present a protocol for an in vitro kinase assay with Cdk1, which allows the identification of Cdk1-specific phosphorylation sites for establishing cellular targets of this important kinase.