In JoVE (3)
- Nanopodia - Thin, Fragile Membrane Projections with Roles in Cell Movement and Intercellular Interactions
- The Modular Design and Production of an Intelligent Robot Based on a Closed-Loop Control Strategy
- A Drosophila In Vivo Injury Model for Studying Neuroregeneration in the Peripheral and Central Nervous System
Other Publications (1)
Articles by Dan Li in JoVE
Nanopodia - Thin, Fragile Membrane Projections with Roles in Cell Movement and Intercellular Interactions Chi-Iou Lin1, Chun-Yee Lau1, Dan Li1, Shou-Ching Jaminet1 1Center for Vascular Biology Research, Department of of Pathology, Beth Israel Deaconess Medical Center, Harvard Medical School Nanopodia are thin but fragile membrane channels that extend up to 100 μm from a cell's leading front or trailing rear and sense the cellular environment. Direct fixation at 37 °C, gentle washing, and avoidance of organic solvents like ethanol, methanol, or acetone and of higher Triton X-100 concentrations are required to observe these cellular structures.
The Modular Design and Production of an Intelligent Robot Based on a Closed-Loop Control Strategy Libo Zhang1, Junjie Zhu2, Hao Ren2, Dongdong Liu3, Dan Meng4, Yanjun Wu1, Tiejian Luo5 1Institute of Software, Chinese Academy of Sciences, 2Hunan University, 3Fuyang Normal University, 4Anhui Normal University, 5University of Chinese Academy of Sciences We present a protocol on modular design and production of intelligent robots to help scientific and technical workers design intelligent robots with special production tasks based on personal needs and individualized design.
A Drosophila In Vivo Injury Model for Studying Neuroregeneration in the Peripheral and Central Nervous System Dan Li*1, Feng Li*1, Pavithran Guttipatti1, Yuanquan Song1,2 1 Here, we present a protocol using the Drosophila sensory neuron - dendritic arborization (da) neuron injury model, which combines in vivo live imaging, two-photon laser axotomy/dendriotomy, and the powerful fly genetic toolbox, as a platform for screening potential promoters and inhibitors of neuroregeneration.
Other articles by Dan Li on PubMed
Liver X Receptors (LXRs) Regulate Apolipoprotein AIV-implications of the Antiatherosclerotic Effect of LXR Agonists Molecular Endocrinology (Baltimore, Md.). Aug, 2004 | Pubmed ID: 15131258 Liver X receptors (LXRs) regulate target genes that are critical in lipoprotein metabolism and atherosclerosis. Apolipoprotein AIV (ApoAIV) is an apolipoprotein that is associated with chylomicrons and high-density lipoproteins. Plasma ApoAIV level in humans is inversely correlated with coronary artery events and overexpression of ApoAIV in mice results in significant reduction in atherosclerosis. We report here that LXRs directly regulate apoAIV at the transcriptional level. Treatment of C57B6 mice with a synthetic LXR agonist, T0901317, resulted in significant increases in plasma apoAIV that was associated with high-density lipoprotein. Examination of both intestinal and liver apoAIV mRNA revealed specific increases in liver mRNA only. In a human heptoma HepG2 cell model, apoAIV mRNA was up-regulated upon the treatment with either native or synthetic LXR agonists. Nuclear run-on study revealed a significant increase in the ApoAIV transcriptional rate upon LXR activation. Examination of the human apoAIV proximal promoter revealed a potential LXR response element that demonstrated binding with HepG2 nuclear extracts. Cotransfection studies in HepG2 cells indicated that this responsive element was functional in mediating the human ApoAIV gene response to LXR agonists. In addition, we identified a functional LXR-responsive element at 3' end enhancer region of mouse ApoAIV gene. We conclude that ApoAIV is a direct target gene of LXRs that may contribute to the antiatherogenic effect of LXR activation.