In JoVE (1)
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Articles by David C. DeWitt in JoVE
Extraction of Hemocytes from Drosophila melanogaster Larvae for Microbial Infection and Analysis Aoi Hiroyasu1, David C. DeWitt2, Alan G. Goodman1 1School of Molecular Biosciences, College of Veterinary Medicine, Washington State University, 2Integrative Physiology and Neuroscience, College of Veterinary Medicine, Washington State University This method demonstrates how to visualize pathogen invasion into insect cells with three-dimensional (3D) models. Hemocytes from Drosophila larvae were infected with viral or bacterial pathogens, either ex vivo or in vivo. Infected hemocytes were then fixed and stained for imaging with a confocal microscope and subsequent 3D cellular reconstruction.