Articles by Davide Carone in JoVE
Optimized System for Cerebral Perfusion Monitoring in the Rat Stroke Model of Intraluminal Middle Cerebral Artery Occlusion Simone Beretta1, Matteo Riva1, Davide Carone1, Elisa Cuccione1, Giada Padovano1, Virginia Rodriguez Menendez1, Giovanni B. Pappadá1, Alessandro Versace1, Carlo Giussani1, Erik P. Sganzerla1, Carlo Ferrarese1 1Department of Neuroscience and Biomedical Technologies, University of Milano Bicocca Cerebral perfusion monitoring has been demonstrated to improve accuracy in ischemic stroke models. Technical difficulties often limit the use of this essential tool for cerebrovascular research. In this video, an optimized system is shown to obtain a single or multi-site hemodynamic monitoring during intraluminal middle cerebral artery occlusion in rats.
Other articles by Davide Carone on PubMed
Hemodynamic Monitoring of Intracranial Collateral Flow Predicts Tissue and Functional Outcome in Experimental Ischemic Stroke Experimental Neurology. Feb, 2012 | Pubmed ID: 22193110 Intracranial collaterals provide residual blood flow to penumbral tissue in acute ischemic stroke and contribute to infarct size variability in humans. In the present study, hemodynamic monitoring of the borderzone territory between the leptomeningeal branches of middle cerebral artery and anterior cerebral artery was compared to lateral middle cerebral artery territory, during common carotid artery occlusion and middle cerebral artery occlusion in rats. The functional performance of intracranial collaterals, shown by perfusion deficit in the territory of leptomeningeal branches either during common carotid artery occlusion or middle cerebral artery occlusion, showed significant variability among animals and consistently predicted infarct size and functional deficit. Our findings indicate that leptomeningeal collateral flow is a strong predictor of stroke severity in rats, similarly to humans. Monitoring of collateral blood flow in experimental stroke is essential for reducing variability in neuroprotection studies and accelerating the development of collateral therapeutics.