In JoVE (1)
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Articles by Dirk F. Hockemeyer in JoVE
Establishment of Genome-edited Human Pluripotent Stem Cell Lines: From Targeting to Isolation John D. Blair1, Helen S. Bateup1, Dirk F. Hockemeyer1 1Department of Molecular and Cell Biology, University of California, Berkeley Genome editing of human pluripotent stem cells (hPSCs) can be done quickly and efficiently. Presented here is a robust experimental procedure to genetically engineer hPSCs as exemplified by editing the AAVS1 safe harbor locus to express EGFP and introduce antibiotic resistance.