In JoVE (1)
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Articles by Dmitry A. Molotkov in JoVE
Gene Delivery to Postnatal Rat Brain by Non-ventricular Plasmid Injection and Electroporation Dmitry A. Molotkov1, Alexey Y. Yukin1,2, Ramil A. Afzalov1,2, Leonard S. Khiroug1 1Neuroscience Center, University of Helsinki, 2Faculty of Biological and Enviromental Sciences, University of Helsinki This protocol describes a non-viral method of delivery of genetic constructs to a certain area of living rodent brain. The method consists of plasmid preparation, micropipette fabrication, neonatal rat pup surgery, microinjection of the construct, and in vivo electroporation.