Articles by Dosh Whye in JoVE
Efficient Differentiation of Mouse Embryonic Stem Cells into Motor Neurons Chia-Yen Wu1, Dosh Whye1, Robert W. Mason1, Wenlan Wang1 1Nemours Biomedical Research, Alfred I. duPont Hospital for Children We developed a new protocol to improve efficiency of in vitro differentiation of mouse embryonic stem cells into motor neurons. The differentiated ES cells acquired motor neurons features as evidenced by expression of neuronal and motor neuron markers using immunohistochemical techniques.
Other articles by Dosh Whye on PubMed
Targeting Nonclassical Oncogenes for Therapy in T-ALL Cancer Cell. Apr, 2012 | Pubmed ID: 22516257 Constitutive phosphoinositide 3-kinase (PI3K)/Akt activation is common in T cell acute lymphoblastic leukemia (T-ALL). Although four distinct class I PI3K isoforms (α, β, γ, δ) could participate in T-ALL pathogenesis, none has been implicated in this process. We report that in the absence of PTEN phosphatase tumor suppressor function, PI3Kγ or PI3Kδ alone can support leukemogenesis, whereas inactivation of both isoforms suppressed tumor formation. The reliance of PTEN null T-ALL on the combined activities of PI3Kγ/δ was further demonstrated by the ability of a dual inhibitor to reduce disease burden and prolong survival in mice as well as prevent proliferation and promote activation of proapoptotic pathways in human tumors. These results support combined inhibition of PI3Kγ/δ as therapy for T-ALL.