Other Publications (1)
Articles by Ekaterina O. Gubernatorova in JoVE
Murine Model of Intestinal Ischemia-reperfusion Injury Ekaterina O. Gubernatorova1,2, Ernesto Perez-Chanona3, Ekaterina P. Koroleva1, Christian Jobin3, Alexei V. Tumanov1,2 1Trudeau Institute, 2Engelhardt Institute of Molecular Biology, 3Departments of Medicine and Infectious Diseases and Pathology, University of Florida Here we describe the detailed procedure of intestinal ischemia-reperfusion in mice which results in reproducible injury without mortality to encourage the standardization of this technique across the field. This model of intestinal ischemia-reperfusion injury can be utilized to study the cellular and molecular mechanisms of injury and regeneration.
Other articles by Ekaterina O. Gubernatorova on PubMed
Citrobacter Rodentium-induced Colitis: A Robust Model to Study Mucosal Immune Responses in the Gut Journal of Immunological Methods. Jun, 2015 | Pubmed ID: 25702536 Citrobacter rodentium is a natural mouse pathogen which reproducibly infects mice and causes intestinal disease. The C. rodentium model of infection is very useful for investigating host-pathogen immune interactions in the gut, and can also be used to understand the pathogenesis of several important human intestinal disorders, including Crohn's disease, ulcerative colitis, dysbiosis and colon tumorigenesis. Both innate and adaptive immune responses play a critical role in protection against C. rodentium. Here, we summarize the role of immune components in protection against C. rodentium and describe techniques for the analysis of innate and adaptive mucosal immune responses, including setting up the infection, analysis of colonic hyperplasia and bacterial dissemination, evaluation of antibody responses, and purification and analysis of intestinal epithelial and lymphoid cells.