Articles by Elçin Ünal in JoVE
Single Molecule Fluorescence In Situ Hybridization (smFISH) Analysis in Budding Yeast Vegetative Growth and Meiosis Jingxun Chen1, David McSwiggen2, Elçin Ünal1 1Department of Molecular and Cell Biology, Barker Hall, University of California, Berkeley, 2Department of Molecular and Cell Biology, Li Ka Shing Center, University of California, Berkeley This single molecule fluorescence in situ hybridization protocol is optimized to quantify the number of RNA molecules in budding yeast during vegetative growth and meiosis.
Other articles by Elçin Ünal on PubMed
One-two Punch Mechanism of Gene Repression: a Fresh Perspective on Gene Regulation Current Genetics. Jun, 2018 | Pubmed ID: 29218463 Cellular differentiation depends on temporally controlled waves of gene activation and inactivation that ultimately transform one cell type into another. It is well established that transcription factor cascades coordinate the timely activation of gene expression clusters during development. In comparison, much less is understood about how gene repression events are coordinated with the transcription factor-driven waves of gene activation and how this repression is achieved at a mechanistic level. Using budding yeast as a model, we recently discovered a new gene regulatory event, whereby a central meiotic transcription factor induces the expression of an mRNA isoform to repress gene expression through an integrated transcriptional and translational mechanism. This new model could explain how gene activation and inactivation waves can be temporally coordinated. In this review, we discuss our findings and their potential implications.