In JoVE (1)
Other Publications (1)
Articles by Elias Uhlin in JoVE
Integration Free Derivation of Human Induced Pluripotent Stem Cells Using Laminin 521 Matrix Elias Uhlin1, Ana Marin Navarro1,2, Harriet Rönnholm1, Kelly Day1, Malin Kele1, Anna Falk1 1Department of Neuroscience, Karolinska Institutet, 2Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet Robust derivation of human induced pluripotent stem (hiPS) cells was achieved by using non-integrating Sendai virus (SeV) vector mediated reprogramming of dermal fibroblasts. hiPS cell maintenance and clonal expansion was performed using xeno-free and chemically defined culture conditions with recombinant human laminin 521 (LN-521) matrix and Essential E8 (E8) Medium.
Other articles by Elias Uhlin on PubMed
Derivation of Human IPS Cell Lines from Monozygotic Twins in Defined and Xeno Free Conditions Stem Cell Research. Jan, 2017 | Pubmed ID: 28395796 Human induced pluripotent stem (hiPS) cell lines CTRL-9-II and CTRL-10-I were derived from healthy monozygotic twin donors using non-integrating RNA based Sendai virus reprogramming and cultured in a xeno-free chemically defined condition. The established hiPS cell lines, CTRL-9-II and CTRL-10-I, are karyotypically normal, free from reprogramming vectors, display endogenously expression of pluripotency factors at levels similar to embryonic stem cells. The generated iPS cell lines demonstrate pluripotency by passing bioinformatics assay PluriTest and by embryonic body assay.