Other Publications (1)
Articles by Ellie Graeden in JoVE
Live avbildning av Zebrafish embryonal hjärna av konfokalmikroskopi Ellie Graeden1,2, Hazel Sive1,2 1Department of Biology, MIT - Massachusetts Institute of Technology, 2Whitehead Institute for Biomedical Research, MIT - Massachusetts Institute of Technology I denna video visar vi en metod för att analysera utvecklingen av ryggradsdjur hjärnan i levande zebrafisk embryon till en enda cell upplösning av konfokalmikroskopi. Detta inkluderar den metod som vi injicerar encelliga zebrafisk embryo och därefter montera och bild den växande hjärnan.
Other articles by Ellie Graeden on PubMed
Formation of the Zebrafish Midbrain-hindbrain Boundary Constriction Requires Laminin-dependent Basal Constriction Mechanisms of Development. Nov-Dec, 2008 | Pubmed ID: 18682291 The midbrain-hindbrain boundary (MHB) is a highly conserved fold in the vertebrate embryonic brain. We have termed the deepest point of this fold the MHB constriction (MHBC) and have begun to define the mechanisms by which it develops. In the zebrafish, the MHBC is formed soon after neural tube closure, concomitant with inflation of the brain ventricles. The MHBC is unusual, as it forms by bending the basal side of the neuroepithelium. At single cell resolution, we show that zebrafish MHBC formation involves two steps. The first is a shortening of MHB cells to approximately 75% of the length of surrounding cells. The second is basal constriction, and apical expansion, of a small group of cells that contribute to the MHBC. In the absence of inflated brain ventricles, basal constriction still occurs, indicating that the MHBC is not formed as a passive consequence of ventricle inflation. In laminin mutants, basal constriction does not occur, indicating an active role for the basement membrane in this process. Apical expansion also fails to occur in laminin mutants, suggesting that apical expansion may be dependent on basal constriction. This study demonstrates laminin-dependent basal constriction as a previously undescribed molecular mechanism for brain morphogenesis.