Articles by Eric B. Berens in JoVE
A Cancer Cell Spheroid Assay to Assess Invasion in a 3D Setting Eric B. Berens1, Jon M. Holy2, Anna T. Riegel1, Anton Wellstein1 1Lombardi Comprehensive Cancer Center, Department of Oncology, Georgetown University, 2Department of Biomedical Sciences, University of Minnesota This method evaluates cancer cell invasion from spheroids into a surrounding 3D matrix. Spheroids are generated via the hanging drop culture method and then embedded in a matrix comprised of basement membrane materials and type I collagen. Invasion out of the spheroids is subsequently monitored.
Testing the Vascular Invasive Ability of Cancer Cells in Zebrafish (Danio Rerio) Eric B. Berens1, Ghada M. Sharif1, Anton Wellstein1, Eric Glasgow1 1Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University This method utilizes zebrafish embryos to efficiently test the vascular invasive ability of cancer cells. Fluorescent cancer cells are injected into the precardiac sinus or yolk sac of developing embryos. Cancer cell vascular invasion and extravasation is assessed via fluorescence microscopy of the tail region 24 to 96 hr later.
Other articles by Eric B. Berens on PubMed
Matriptase and Prostasin Are Expressed in Human Skin in an Inverse Trend over the Course of Differentiation and Are Targeted to Different Regions of the Plasma Membrane Biology Open. Oct, 2016 | Pubmed ID: 27543057 Matriptase and prostasin, acting as a tightly coupled proteolytic cascade, were reported to be required for epidermal barrier formation in mouse skin. Here we show that, in human skin, matriptase and prostasin are expressed with an inverse pattern over the course of differentiation. Matriptase was detected primarily in epidermal basal keratinocytes and the basaloid cells in the outer root sheath of hair follicles and the sebaceous gland, where prostasin was not detected. In contrast, prostasin was detected primarily in differentiated cells in the epidermal granular layer, the inner root sheath of hair follicles, and the sebaceous gland, where matriptase expression is negligible. While co-expressed in the middle stage of differentiation, prostasin was detected as polarized patches, and matriptase at intercellular junctions. Targeting to different subcellular localizations is also observed in HaCaT human keratinocytes, in which matriptase was detected primarily at intercellular junctions, and prostasin primarily on membrane protrusion. Furthermore, upon induction of zymogen activation, free active prostasin remains cell-associated and free active matriptase is rapidly shed into the extracellular milieu. Our data suggest that matriptase and prostasin likely function as independent entities in human skin rather than as a tightly coupled proteolytic cascade as observed in mouse skin.