Articles by Eric K. Hubner in JoVE
Constitutive and Inducible Systems for Genetic In Vivo Modification of Mouse Hepatocytes Using Hydrodynamic Tail Vein Injection Eric K. Hubner1,2, Christian Lechler1, Thomas N. Rösner1, Birgit Kohnke-Ertel1, Roland M. Schmid1, Ursula Ehmer1 1Department of Medicine II, Klinikum rechts der Isar, Technische Universität München, 2Department of Pneumology, Center for Medicine, Medical Center University of Freiburg Hydrodynamic tail vein injection of transposon-based integration vectors enables stable transfection of murine hepatocytes in vivo. Here, we present a practical protocol for transfection systems that enables the long-term constitutive expression of a single transgene or combined constitutive and doxycycline-inducible expression of a transgene or miR-shRNA in the liver.
Other articles by Eric K. Hubner on PubMed
An in Vivo Transfection System for Inducible Gene Expression and Gene Silencing in Murine Hepatocytes The Journal of Gene Medicine. | Pubmed ID: 28009940 Hydrodynamic tail vein injection (HTVI) of transposon-based integration vectors is an established system for stably transfecting mouse hepatocytes in vivo that has been successfully employed to study key questions in liver biology and cancer. Refining the vectors for transposon-mediated hepatocyte transfection will further expand the range of applications of this technique in liver research. In the present study, we report an advanced transposon-based system for manipulating gene expression in hepatocytes in vivo.