Articles by Erin T. Filbrandt in JoVE
Generation of Human Nasal Epithelial Cell Spheroids for Individualized Cystic Fibrosis Transmembrane Conductance Regulator Study John J. Brewington1, Erin T. Filbrandt1, Francis J. LaRosa III1, Jessica D. Moncivaiz1, Alicia J. Ostmann1, Lauren M. Strecker1, John P. Clancy1 1 Here we describe a method to generate three-dimensional spheroid cultures of human nasal epithelial cells. Spheroids are then stimulated to drive Cystic Fibrosis Transmembrane Conductance Regulator (CFTR)-dependent ion and fluid secretion, quantifying the change in the spheroid luminal size as a proxy for CFTR function.
Other articles by Erin T. Filbrandt on PubMed
Detection of CFTR Function and Modulation in Primary Human Nasal Cell Spheroids Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society. | Pubmed ID: 28712885 Expansion of CFTR modulators to patients with rare/undescribed mutations will be facilitated by patient-derived models quantifying CFTR function and restoration. We aimed to generate a personalized model system of CFTR function and modulation using non-surgically obtained nasal epithelial cells (NECs).