In JoVE (1)
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Articles by Eshita Khera in JoVE
Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability Hui Gao1, Eshita Khera2, Jung-Kul Lee1, Fei Wen2 1Department of Chemical Engineering, Konkuk University, 2Department of Chemical Engineering, University of Michigan Presented is the protocol for co-immobilizing whole-cell biocatalysts for cofactor regeneration and improved reusability, using the production of L-xylulose as an example. The cofactor regeneration is achieved by coupling two Escherichia coli strains expressing functionally complementary enzymes; the whole-cell biocatalyst immobilization is achieved by cell encapsulation in calcium alginate beads.