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Articles by Ewa Molska in JoVE
Investigation of RNA Synthesis Using 5-Bromouridine Labelling and Immunoprecipitation Rikke H. Kofoed1, Cristine Betzer1, Søren Lykke-Andersen2, Ewa Molska2, Poul H. Jensen1 1DANDRITE - Danish Research Institute of Translational Neuroscience, Dept. of Biomedicine, Aarhus University, 2Dept. of Molecular Biology and Genetics, Aarhus University This method can be used to measure RNA synthesis. 5-Bromouridine is added to cells and incorporated into synthesized RNA. RNA synthesis is measured by RNA extraction immediately after labelling, followed by 5-Bromouridine-targeted immunoprecipitation of labelled RNA and analysis by reverse transcription and quantitative polymerase chain reaction.