Fanrong Kong Centre for Infectious Diseases and Microbiology University of Sydney Biography Publications Institution JoVE Articles Fanrong Kong has not added a biography. If you are Fanrong Kong and would like to personalize this page please email our Author Liaison for assistance. Publications Multiplex PCR-Based Reverse Line Blot Assay for Simultaneous Detection of 22 Virulence Genes in Uropathogenic Escherichia Coli Applied and Environmental Microbiology. Feb, 2012 | Pubmed ID: 22156422 Three-locus Identification, Genotyping, and Antifungal Susceptibilities of Medically Important Trichosporon Species from China Journal of Clinical Microbiology. Nov, 2011 | Pubmed ID: 21900517 Rapid Detection of Isoniazid, Rifampin, and Ofloxacin Resistance in Mycobacterium Tuberculosis Clinical Isolates Using High-resolution Melting Analysis Journal of Clinical Microbiology. Oct, 2011 | Pubmed ID: 21832014 Defining Reference Sequences for Nocardia Species by Similarity and Clustering Analyses of 16S RRNA Gene Sequence Data PloS One. 2011 | Pubmed ID: 21687706 Genotyping of Human Adenoviruses Using a PCR-based Reverse Line Blot Hybridisation Assay Pathology. Aug, 2011 | Pubmed ID: 21670723 Computational Bacterial Genome-wide Analysis of Phylogenetic Profiles Reveals Potential Virulence Genes of Streptococcus Agalactiae PloS One. 2011 | Pubmed ID: 21483735 Contributions of Aromatic Pairs to the Folding and Stability of Long-lived Human γD-crystallin Protein Science : a Publication of the Protein Society. Mar, 2011 | Pubmed ID: 21432932 The Prevalence of Urogenital Micro-organisms Detected by a Multiplex PCR-reverse Line Blot Assay in Women Attending Three Sexual Health Clinics in Sydney, Australia Journal of Medical Microbiology. Jul, 2011 | Pubmed ID: 21415210 Accurate and Practical Identification of 20 Fusarium Species by Seven-locus Sequence Analysis and Reverse Line Blot Hybridization, and an in Vitro Antifungal Susceptibility Study Journal of Clinical Microbiology. May, 2011 | Pubmed ID: 21389150 Improved Identification of Gordonia, Rhodococcus and Tsukamurella Species by 5'-end 16S RRNA Gene Sequencing Pathology. Jan, 2011 | Pubmed ID: 21240067 Contributions of Aromatic Pairs to the Folding and Stability of Long-lived Human γD-crystallin Protein Science : a Publication of the Protein Society. Jan, 2011 | Pubmed ID: 21213250 Molecular Characterization of Enterovirus 71 and Coxsackievirus A16 Using the 5' Untranslated Region and VP1 Region Journal of Medical Microbiology. Mar, 2011 | Pubmed ID: 21148280 SecA1 Gene Sequence Polymorphisms for Species Identification of Nocardia Species and Recognition of Intraspecies Genetic Diversity Journal of Clinical Microbiology. Nov, 2010 | Pubmed ID: 20810768 Unexpected Diversity of Staphylococcal Cassette Chromosome Mec Type IV in Methicillin-resistant Staphylococcus Aureus Strains Journal of Clinical Microbiology. Oct, 2010 | Pubmed ID: 20686095 Rapid Identification of Methicillin-resistant Staphylococcus Aureus Transmission in Hospitals by Use of Phage-derived Open Reading Frame Typing Enhanced by Multiplex PCR and Reverse Line Blot Assay Journal of Clinical Microbiology. Aug, 2010 | Pubmed ID: 20519463 Molecular Identification and Analysis of Nonserotypeable Human Enteroviruses Journal of Clinical Microbiology. Apr, 2010 | Pubmed ID: 20164278 Reverse Line Blot Hybridization and DNA Sequencing Studies of the 16S-23S RRNA Gene Intergenic Spacer Regions of Five Emerging Pathogenic Nocardia Species Journal of Medical Microbiology. May, 2010 | Pubmed ID: 20110385 Distribution of Serotypes, Genotypes, and Resistance Determinants Among Macrolide-resistant Streptococcus Pneumoniae Isolates Antimicrobial Agents and Chemotherapy. Mar, 2010 | Pubmed ID: 20065057 Identification of Pathogenic Nocardia Species by Reverse Line Blot Hybridization Targeting the 16S RRNA and 16S-23S RRNA Gene Spacer Regions Journal of Clinical Microbiology. Feb, 2010 | Pubmed ID: 19955277 Commonly Used Molecular Epidemiology Markers of Streptococcus Agalactiae Do Not Appear to Predict Virulence Pathology. 2009 | Pubmed ID: 19900108 First Report of Putative Streptococcus Pneumoniae Serotype 6D Among Nasopharyngeal Isolates from Fijian Children The Journal of Infectious Diseases. Nov, 2009 | Pubmed ID: 19803727 Rapid Detection of ERG11 Gene Mutations in Clinical Candida Albicans Isolates with Reduced Susceptibility to Fluconazole by Rolling Circle Amplification and DNA Sequencing BMC Microbiology. 2009 | Pubmed ID: 19682357 Assignment of Reference 5'-end 16S RDNA Sequences and Species-Specific Sequence Polymorphisms Improves Species Identification of Nocardia The Open Microbiology Journal. 2009 | Pubmed ID: 19639036 Rolling Circle Amplification and Multiplex Allele-specific PCR for Rapid Detection of KatG and InhA Gene Mutations in Mycobacterium Tuberculosis International Journal of Medical Microbiology : IJMM. Dec, 2009 | Pubmed ID: 19604720 Identification of Surface Proteins of Group B Streptococci: Serotyping Versus Genotyping Journal of Microbiological Methods. Sep, 2009 | Pubmed ID: 19573567 Identification of 20 Common Human Enterovirus Serotypes by Use of a Reverse Transcription-PCR-based Reverse Line Blot Hybridization Assay Journal of Clinical Microbiology. Sep, 2009 | Pubmed ID: 19571022 Simple, Accurate, Serotype-specific PCR Assay to Differentiate Streptococcus Pneumoniae Serotypes 6A, 6B, and 6C Journal of Clinical Microbiology. Aug, 2009 | Pubmed ID: 19535528 A New Multiplex PCR-based Reverse Line-blot Hybridization (mPCR/RLB) Assay for Rapid Staphylococcal Cassette Chromosome Mec (SCCmec) Typing Journal of Medical Microbiology. Aug, 2009 | Pubmed ID: 19528184 Diversity of Group B Streptococcus Serotypes Causing Urinary Tract Infection in Adults Journal of Clinical Microbiology. Jul, 2009 | Pubmed ID: 19439533 Simultaneous Identification of 14 Genital Microorganisms in Urine by Use of a Multiplex PCR-based Reverse Line Blot Assay Journal of Clinical Microbiology. Jun, 2009 | Pubmed ID: 19357202 Integron Gene Cassettes in Acinetobacter Spp. Strains from South China International Journal of Antimicrobial Agents. Nov, 2008 | Pubmed ID: 18757181 Assignment of Streptococcus Agalactiae Isolates to Clonal Complexes Using a Small Set of Single Nucleotide Polymorphisms BMC Microbiology. 2008 | Pubmed ID: 18710585 Hyperbranched Rolling Circle Amplification As a Rapid and Sensitive Method for Species Identification Within the Cryptococcus Species Complex Electrophoresis. Aug, 2008 | Pubmed ID: 18600831 Extended Phage Locus Typing of Salmonella Enterica Serovar Typhimurium, Using Multiplex PCR-based Reverse Line Blot Hybridization Journal of Medical Microbiology. Jul, 2008 | Pubmed ID: 18566140 Use of Phenotypic and Molecular Serotype Identification Methods to Characterize Previously Nonserotypeable Group B Streptococci Journal of Clinical Microbiology. Aug, 2008 | Pubmed ID: 18562579 Practical Method for Detection and Identification of Candida, Aspergillus, and Scedosporium Spp. by Use of Rolling-circle Amplification Journal of Clinical Microbiology. Jul, 2008 | Pubmed ID: 18495860 Rapid Identification and Differentiation of Trichophyton Species, Based on Sequence Polymorphisms of the Ribosomal Internal Transcribed Spacer Regions, by Rolling-circle Amplification Journal of Clinical Microbiology. Apr, 2008 | Pubmed ID: 18234865 A Multiplex PCR-based Reverse Line Blot Hybridization (mPCR/RLB) Assay for Detection of Bacterial Respiratory Pathogens in Children with Pneumonia Pediatric Pulmonology. Feb, 2008 | Pubmed ID: 18085683 Agar Dilution Method for Detection of Inducible Clindamycin Resistance in Staphylococcus Spp Journal of Clinical Microbiology. Dec, 2007 | Pubmed ID: 17942656 Phase Studies of Model Biomembranes: Macroscopic Coexistence of Lalpha+Lbeta, with Light-induced Coexistence of Lalpha+Lo Phases Biochimica Et Biophysica Acta. Nov, 2007 | Pubmed ID: 17931595 Comparison of Single- and Multilocus Sequence Typing and Toxin Gene Profiling for Characterization of Methicillin-resistant Staphylococcus Aureus Journal of Clinical Microbiology. Oct, 2007 | Pubmed ID: 17715374 Identification of Less-common Streptococcus Pneumoniae Serotypes by a Multiplex PCR-based Reverse Line Blot Hybridization Assay Journal of Clinical Microbiology. Oct, 2007 | Pubmed ID: 17687009 Reverse Line Blot Hybridization Assay for Identification of Medically Important Fungi from Culture and Clinical Specimens Journal of Clinical Microbiology. Sep, 2007 | Pubmed ID: 17634313 Serotype IX, a Proposed New Streptococcus Agalactiae Serotype Journal of Clinical Microbiology. Sep, 2007 | Pubmed ID: 17634306 Three New Macrolide Efflux (mef) Gene Variants in Streptococcus Agalactiae Journal of Clinical Microbiology. Aug, 2007 | Pubmed ID: 17596365 In Vitro Activities of Miltefosine and Two Novel Antifungal Biscationic Salts Against a Panel of 77 Dermatophytes Antimicrobial Agents and Chemotherapy. Jun, 2007 | Pubmed ID: 17371821 Development of a DNA Microarray to Identify the Streptococcus Pneumoniae Serotypes Contained in the 23-valent Pneumococcal Polysaccharide Vaccine and Closely Related Serotypes Journal of Microbiological Methods. Jan, 2007 | Pubmed ID: 16904781 Multiplex PCR-based Reverse Line Blot Hybridization Assay (mPCR/RLB)--a Practical Epidemiological and Diagnostic Tool Nature Protocols. 2006 | Pubmed ID: 17406523 Evaluation of a Multiplex PCR-based Reverse Line Blot-hybridization Assay for Identification of Serotype and Surface Protein Antigens of Streptococcus Agalactiae Journal of Clinical Microbiology. Oct, 2006 | Pubmed ID: 17021119 Use of PCR and Reverse Line Blot Hybridization Macroarray Based on 16S-23S RRNA Gene Internal Transcribed Spacer Sequences for Rapid Identification of 34 Mycobacterium Species Journal of Clinical Microbiology. Oct, 2006 | Pubmed ID: 17021080 Identification of a Streptococcus Agalactiae Serotype III Subtype 4 Clone in Association with Adult Invasive Disease in Hong Kong Journal of Clinical Microbiology. Nov, 2006 | Pubmed ID: 17005749 Influence of Disk Separation Distance on Accuracy of the Disk Approximation Test for Detection of Inducible Clindamycin Resistance in Staphylococcus Spp Journal of Clinical Microbiology. Nov, 2006 | Pubmed ID: 17005747 Molecular Serotype Identification of Streptococcus Agalactiae of Bovine Origin by Multiplex PCR-based Reverse Line Blot (mPCR/RLB) Hybridization Assay FEMS Microbiology Letters. Oct, 2006 | Pubmed ID: 16978362 Streptococcus Agalactiae Cbeta Protein Gene (bac) Sequence Types, Based on the Repeated Region of the Cell-wall-spanning Domain: Relationship to Virulence and a Proposed Standardized Nomenclature Journal of Medical Microbiology. Jul, 2006 | Pubmed ID: 16772408 Multiplex PCR-based Reverse Line Blot Hybridization Assay to Identify 23 Streptococcus Pneumoniae Polysaccharide Vaccine Serotypes Journal of Clinical Microbiology. May, 2006 | Pubmed ID: 16672432 Use of a Serotype-specific DNA Microarray for Identification of Group B Streptococcus (Streptococcus Agalactiae) Journal of Clinical Microbiology. Apr, 2006 | Pubmed ID: 16597875 Use of PCR and Reverse Line Blot Hybridization Assay for Rapid Simultaneous Detection and Serovar Identification of Chlamydia Trachomatis Journal of Clinical Microbiology. Apr, 2006 | Pubmed ID: 16597870 Simultaneous Detection and Identification of Candida, Aspergillus, and Cryptococcus Species by Reverse Line Blot Hybridization Journal of Clinical Microbiology. Mar, 2006 | Pubmed ID: 16517870 Reverse Line Blot Assay for Direct Identification of Seven Streptococcus Agalactiae Major Surface Protein Antigen Genes Clinical and Vaccine Immunology : CVI. Jan, 2006 | Pubmed ID: 16426012 Simultaneous Detection of Nine Antibiotic Resistance-related Genes in Streptococcus Agalactiae Using Multiplex PCR and Reverse Line Blot Hybridization Assay Antimicrobial Agents and Chemotherapy. Jan, 2006 | Pubmed ID: 16377687 Simultaneous Detection and Serotype Identification of Streptococcus Agalactiae Using Multiplex PCR and Reverse Line Blot Hybridization Journal of Medical Microbiology. Dec, 2005 | Pubmed ID: 16278425 Comparison of a 3-set Genotyping System with Multilocus Sequence Typing for Streptococcus Agalactiae (Group B Streptococcus) Journal of Clinical Microbiology. Sep, 2005 | Pubmed ID: 16145130 A Molecular-capsular-type Prediction System for 90 Streptococcus Pneumoniae Serotypes Using Partial CpsA-cpsB Sequencing and Wzy- or Wzx-specific PCR Journal of Medical Microbiology. Apr, 2005 | Pubmed ID: 15770019 Postgenomic Taxonomy of Human Ureaplasmas -- a Case Study Based on Multiple Gene Sequences International Journal of Systematic and Evolutionary Microbiology. Sep, 2004 | Pubmed ID: 15388749 Simultaneous Detection and Identification of Common Cell Culture Contaminant and Pathogenic Mollicutes Strains by Reverse Line Blot Hybridization Applied and Environmental Microbiology. Mar, 2004 | Pubmed ID: 15006769 Using CpsA-cpsB Sequence Polymorphisms and Serotype-/group-specific PCR to Predict 51 Streptococcus Pneumoniae Capsular Serotypes Journal of Medical Microbiology. Dec, 2003 | Pubmed ID: 14614062 Towards a Genotyping System for Streptococcus Agalactiae (group B Streptococcus): Use of Mobile Genetic Elements in Australasian Invasive Isolates Journal of Medical Microbiology. Apr, 2003 | Pubmed ID: 12676873 Proposal of Ureaplasma Parvum Sp. Nov. and Emended Description of Ureaplasma Urealyticum (Shepard Et Al. 1974) Robertson Et Al. 2001 International Journal of Systematic and Evolutionary Microbiology. Mar, 2002 | Pubmed ID: 11931172 Molecular Profiles of Group B Streptococcal Surface Protein Antigen Genes: Relationship to Molecular Serotypes Journal of Clinical Microbiology. Feb, 2002 | Pubmed ID: 11825981 Serotype Identification of Group B Streptococci by PCR and Sequencing Journal of Clinical Microbiology. Jan, 2002 | Pubmed ID: 11773119 Multiplex PCR and Reverse Line Blot Hybridization Assay (mPCR/RLB) Matthew V. N. O'Sullivan1, Fei Zhou1, Vitali Sintchenko1, Fanrong Kong1, Gwendolyn L. Gilbert1 1Centre for Infectious Diseases and Microbiology, University of Sydney JoVE 2781 Biology
Multiplex PCR and Reverse Line Blot Hybridization Assay (mPCR/RLB) Matthew V. N. O'Sullivan1, Fei Zhou1, Vitali Sintchenko1, Fanrong Kong1, Gwendolyn L. Gilbert1 1Centre for Infectious Diseases and Microbiology, University of Sydney JoVE 2781 Biology