In JoVE (1)
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Articles by Francis J. LaRosa III in JoVE
Generation of Human Nasal Epithelial Cell Spheroids for Individualized Cystic Fibrosis Transmembrane Conductance Regulator Study John J. Brewington1, Erin T. Filbrandt1, Francis J. LaRosa III1, Jessica D. Moncivaiz1, Alicia J. Ostmann1, Lauren M. Strecker1, John P. Clancy1 1 Here we describe a method to generate three-dimensional spheroid cultures of human nasal epithelial cells. Spheroids are then stimulated to drive Cystic Fibrosis Transmembrane Conductance Regulator (CFTR)-dependent ion and fluid secretion, quantifying the change in the spheroid luminal size as a proxy for CFTR function.