Frank Sobott School of Molecular and Cellular Biology, Faculty of Biological Sciences & Astbury Centre for Structural and Molecular Biology University of Leeds Biography Publications Institution JoVE Articles Frank Sobott has not added a biography. If you are Frank Sobott and would like to personalize this page please email our Author Liaison for assistance. Publications Nanobody-aided Crystallization of the Transcription Regulator PaaR2 from Escherichia Coli O157:H7 Acta Crystallographica. Section F, Structural Biology Communications. Oct, 2021 | Pubmed ID: 34605442 On-grid and In-flow Mixing for Time-resolved Cryo-EM Acta Crystallographica. Section D, Structural Biology. Oct, 2021 | Pubmed ID: 34605427 Analysis of the PcrA-RNA Polymerase Complex Reveals a Helicase Interaction Motif and a Role for PcrA/UvrD Helicase in the Suppression of R-loops ELife. 07, 2021 | Pubmed ID: 34279225 Profiling Dopamine-Induced Oxidized Proteoforms of β-synuclein by Top-Down Mass Spectrometry Antioxidants (Basel, Switzerland). Jun, 2021 | Pubmed ID: 34206096 Entropic Pressure Controls the Oligomerization of the Vibrio Cholerae ParD2 Antitoxin Acta Crystallographica. Section D, Structural Biology. Jul, 2021 | Pubmed ID: 34196617 Structure of the Endocytic Adaptor Complex Reveals the Basis for Efficient Membrane Anchoring During Clathrin-mediated Endocytosis Nature Communications. 05, 2021 | Pubmed ID: 34001871 Structural Modeling of a Novel Membrane-bound Globin-coupled Sensor in Computational and Structural Biotechnology Journal. 2021 | Pubmed ID: 33995893 A Model of Full-length RAGE in Complex with S100B Structure (London, England : 1993). Sep, 2021 | Pubmed ID: 33887170 Brazilin Removes Toxic Alpha-Synuclein and Seeding Competent Assemblies from Parkinson Brain by Altering Conformational Equilibrium Journal of Molecular Biology. 04, 2021 | Pubmed ID: 33610557 Unveiling the Binding Mode of Perfluorooctanoic Acid to Human Serum Albumin Protein Science : a Publication of the Protein Society. 04, 2021 | Pubmed ID: 33550662 Cryo-EM Structure of Human Mitochondrial HSPD1 IScience. Jan, 2021 | Pubmed ID: 33506187 Discriminative SKP2 Interactions with CDK-Cyclin Complexes Support a Cyclin A-Specific Role in P27KIP1 Degradation Journal of Molecular Biology. 03, 2021 | Pubmed ID: 33422522 Aptamer-ligand Recognition Studied by Native Ion Mobility-mass Spectrometry Talanta. Mar, 2021 | Pubmed ID: 33379118 Interrogating Membrane Protein Structure and Lipid Interactions by Native Mass Spectrometry Methods in Molecular Biology (Clifton, N.J.). 2020 | Pubmed ID: 33582995 Further Insights from Structural Mass Spectrometry into Endocytosis Adaptor Protein Assemblies International Journal of Mass Spectrometry. Jan, 2020 | Pubmed ID: 33244295 Do Aptamers Always Bind? The Need for a Multifaceted Analytical Approach When Demonstrating Binding Affinity Between Aptamer and Low Molecular Weight Compounds Journal of the American Chemical Society. 11, 2020 | Pubmed ID: 33166132 Effects of Detergent on α-Synuclein Structure. A Native MS-Ion Mobility Study International Journal of Molecular Sciences. Oct, 2020 | Pubmed ID: 33114222 Toward Increased Reliability, Transparency, and Accessibility in Cross-linking Mass Spectrometry Structure (London, England : 1993). 11, 2020 | Pubmed ID: 33065067 Metal Ions Shape α-synuclein Scientific Reports. 10, 2020 | Pubmed ID: 33004902 Need for Speed: Examining Protein Behavior During CryoEM Grid Preparation at Different Timescales Structure (London, England : 1993). 11, 2020 | Pubmed ID: 32814033 Interlaboratory Study for Characterizing Monoclonal Antibodies by Top-Down and Middle-Down Mass Spectrometry Journal of the American Society for Mass Spectrometry. Sep, 2020 | Pubmed ID: 32812765 M42 Aminopeptidase Catalytic Site: the Structural and Functional Role of a Strictly Conserved Aspartate Residue Proteins. 12, 2020 | Pubmed ID: 32673419 Computational Strategies and Challenges for Using Native Ion Mobility Mass Spectrometry in Biophysics and Structural Biology Analytical Chemistry. 08, 2020 | Pubmed ID: 32667808 Software Requirements for the Analysis and Interpretation of Native Ion Mobility Mass Spectrometry Data Analytical Chemistry. 08, 2020 | Pubmed ID: 32649184 Extent of N-terminus Exposure of Monomeric Alpha-synuclein Determines Its Aggregation Propensity Nature Communications. 06, 2020 | Pubmed ID: 32499486 Sample Deposition Onto Cryo-EM Grids: from Sprays to Jets and Back Acta Crystallographica. Section D, Structural Biology. Apr, 2020 | Pubmed ID: 32254058 Allosteric Modulation of the GTPase Activity of a Bacterial LRRK2 Homolog by Conformation-specific Nanobodies The Biochemical Journal. 04, 2020 | Pubmed ID: 32167135 Styrene Maleic-acid Lipid Particles (SMALPs) into Detergent or Amphipols: An Exchange Protocol for Membrane Protein Characterisation Biochimica Et Biophysica Acta. Biomembranes. 05, 2020 | Pubmed ID: 31945320 The Escherichia Coli RnlA-RnlB Toxin-antitoxin Complex: Production, Characterization and Crystallization Acta Crystallographica. Section F, Structural Biology Communications. Jan, 2020 | Pubmed ID: 31929184 Enhanced Oligomerization of Full-length RAGE by Synergy of the Interaction of Its Domains Scientific Reports. 12, 2019 | Pubmed ID: 31889156 A Cryo-EM Grid Preparation Device for Time-resolved Structural Studies IUCrJ. Nov, 2019 | Pubmed ID: 31709058 How Metal Cofactors Drive Dimer-dodecamer Transition of the M42 Aminopeptidase TmPep1050 of The Journal of Biological Chemistry. Nov, 2019 | Pubmed ID: 31611236 Structural Mechanism of Synergistic Activation of Aurora Kinase B/C by Phosphorylated INCENP Nature Communications. 07, 2019 | Pubmed ID: 31320618 MIND: A Double-Linear Model To Accurately Determine Monoisotopic Precursor Mass in High-Resolution Top-Down Proteomics Analytical Chemistry. 08, 2019 | Pubmed ID: 31283196 Native Ion Mobility-Mass Spectrometry Reveals the Formation of β-Barrel Shaped Amyloid-β Hexamers in a Membrane-Mimicking Environment Journal of the American Chemical Society. 07, 2019 | Pubmed ID: 31141355 First Community-Wide, Comparative Cross-Linking Mass Spectrometry Study Analytical Chemistry. 06, 2019 | Pubmed ID: 31045356 Thermodynamic Stability of the Transcription Regulator PaaR2 from Escherichia Coli O157:H7 Biophysical Journal. 04, 2019 | Pubmed ID: 30979547 Cyclized NDGA Modifies Dynamic α-synuclein Monomers Preventing Aggregation and Toxicity Scientific Reports. 02, 2019 | Pubmed ID: 30814575 A Dual Role in Regulation and Toxicity for the Disordered N-terminus of the Toxin GraT Nature Communications. 02, 2019 | Pubmed ID: 30814507 Methionine Oxidation in α-synuclein Inhibits Its Propensity for Ordered Secondary Structure The Journal of Biological Chemistry. 04, 2019 | Pubmed ID: 30755483 Recommendations for Reporting Ion Mobility Mass Spectrometry Measurements Mass Spectrometry Reviews. 05, 2019 | Pubmed ID: 30707468 Masstodon: A Tool for Assigning Peaks and Modeling Electron Transfer Reactions in Top-Down Mass Spectrometry Analytical Chemistry. 02, 2019 | Pubmed ID: 30608646 Editorial and Review: 30th ASMS Sanibel Conference on Mass Spectrometry-Computational Modelling in Mass Spectrometry and Ion Mobility: Methods for Ion Structure and Reactivity Determination Journal of the American Society for Mass Spectrometry. Dec, 2018 | Pubmed ID: 30397862 The Effect of Reactive Oxygen and Nitrogen Species on the Structure of Cytoglobin: A Potential Tumor Suppressor Redox Biology. 10, 2018 | Pubmed ID: 30081385 Phosphorylation Decelerates Conformational Dynamics in Bacterial Translation Elongation Factors Science Advances. 03, 2018 | Pubmed ID: 29546243 Radical Solutions: Principles and Application of Electron-based Dissociation in Mass Spectrometry-based Analysis of Protein Structure Mass Spectrometry Reviews. 11, 2018 | Pubmed ID: 29425406 Fixed-Charge Trimethyl Pyrilium Modification for Enabling Enhanced Top-Down Mass Spectrometry Sequencing of Intact Protein Complexes Analytical Chemistry. 02, 2018 | Pubmed ID: 29360341 Evaluation of Ion Mobility for the Separation of Glycoconjugate Isomers Due to Different Types of Sialic Acid Linkage, at the Intact Glycoprotein, Glycopeptide and Glycan Level Journal of Proteomics. 02, 2018 | Pubmed ID: 29197583 Analysis of the Natively Unstructured RNA/protein-recognition Core in the Escherichia Coli RNA Degradosome and Its Interactions with Regulatory RNA/Hfq Complexes Nucleic Acids Research. 01, 2018 | Pubmed ID: 29136196 Advances in Native High-performance Liquid Chromatography and Intact Mass Spectrometry for the Characterization of Biopharmaceutical Products Journal of Separation Science. Jan, 2018 | Pubmed ID: 28990739 The Structural Basis for Dynamic DNA Binding and Bridging Interactions Which Condense the Bacterial Centromere ELife. 12, 2017 | Pubmed ID: 29244022 A Homologue of the Parkinson's Disease-associated Protein LRRK2 Undergoes a Monomer-dimer Transition During GTP Turnover Nature Communications. 10, 2017 | Pubmed ID: 29044096 Apparent Activation Energies of Protein-protein Complex Dissociation in the Gas-phase Determined by Electrospray Mass Spectrometry Analytical and Bioanalytical Chemistry. Nov, 2017 | Pubmed ID: 28900708 Native Mass Spectrometry for the Characterization of Structure and Interactions of Membrane Proteins Methods in Molecular Biology (Clifton, N.J.). 2017 | Pubmed ID: 28755371 Specific Sequences in the N-terminal Domain of Human Small Heat-shock Protein HSPB6 Dictate Preferential Hetero-oligomerization with the Orthologue HSPB1 The Journal of Biological Chemistry. 06, 2017 | Pubmed ID: 28487364 AtaT Blocks Translation Initiation by N-acetylation of the Initiator TRNA Nature Chemical Biology. 06, 2017 | Pubmed ID: 28369041 Phase Separation of C9orf72 Dipeptide Repeats Perturbs Stress Granule Dynamics Molecular Cell. Mar, 2017 | Pubmed ID: 28306503 Analyzing Complex Mixtures of Drug-like Molecules: Ion Mobility As an Adjunct to Existing Liquid Chromatography-(tandem) Mass Spectrometry Methods Journal of Chromatography. A. Mar, 2017 | Pubmed ID: 28228233 Proton Dynamics in Protein Mass Spectrometry The Journal of Physical Chemistry Letters. Mar, 2017 | Pubmed ID: 28207277 Conformational Space and Stability of ETD Charge Reduction Products of Ubiquitin Journal of the American Society for Mass Spectrometry. 01, 2017 | Pubmed ID: 27495285 Opposite Structural Effects of Epigallocatechin-3-gallate and Dopamine Binding to α-Synuclein Analytical Chemistry. 09, 2016 | Pubmed ID: 27467405 Combining Density Functional Theory (DFT) and Collision Cross-section (CCS) Calculations to Analyze the Gas-phase Behaviour of Small Molecules and Their Protonation Site Isomers The Analyst. Jun, 2016 | Pubmed ID: 27264846 Fast Grid Preparation for Time-Resolved Cryo-Electron Microscopy David P. Klebl1, Frank Sobott2, Howard D. White3, Stephen P. Muench1 1School of Biomedical Sciences, Faculty of Biological Sciences & Astbury Centre for Structural and Molecular Biology, University of Leeds, 2School of Molecular and Cellular Biology, Faculty of Biological Sciences & Astbury Centre for Structural and Molecular Biology, University of Leeds, 3Department of Physiological Sciences, Eastern Virginia Medical School JoVE 62199 Biochemistry
Fast Grid Preparation for Time-Resolved Cryo-Electron Microscopy David P. Klebl1, Frank Sobott2, Howard D. White3, Stephen P. Muench1 1School of Biomedical Sciences, Faculty of Biological Sciences & Astbury Centre for Structural and Molecular Biology, University of Leeds, 2School of Molecular and Cellular Biology, Faculty of Biological Sciences & Astbury Centre for Structural and Molecular Biology, University of Leeds, 3Department of Physiological Sciences, Eastern Virginia Medical School JoVE 62199 Biochemistry
