Gary R. Whittaker Department of Microbiology and Immunology, College of Veterinary Medicine Cornell University Biography Publications Institution JoVE Articles Gary R. Whittaker has not added a biography. If you are Gary R. Whittaker and would like to personalize this page please email our Author Liaison for assistance. Publications Characterizing Replication Kinetics and Plaque Production of Type I Feline Infectious Peritonitis Virus in Three Feline Cell Lines Virology. 12, 2018 | Pubmed ID: 30205273 Feline Coronavirus: Insights into Viral Pathogenesis Based on the Spike Protein Structure and Function Virology. 04, 2018 | Pubmed ID: 29329682 Improving Virus Taxonomy by Recontextualizing Sequence-Based Classification with Biologically Relevant Data: the Case of the Species MSphere. Jan-Feb, 2018 | Pubmed ID: 29299531 Physiological and Molecular Triggers for SARS-CoV Membrane Fusion and Entry into Host Cells Virology. 04, 2018 | Pubmed ID: 29275820 The SARS-CoV Fusion Peptide Forms an Extended Bipartite Fusion Platform That Perturbs Membrane Order in a Calcium-Dependent Manner Journal of Molecular Biology. Dec, 2017 | Pubmed ID: 29056462 A Single Dose and Long Lasting Vaccine Against Pandemic Influenza Through the Controlled Release of a Heterospecies Tandem M2 Sequence Embedded Within Detoxified Bacterial Outer Membrane Vesicles Vaccine. 09, 2017 | Pubmed ID: 28866291 Use of AAScatterPlot Tool for Monitoring the Evolution of the Hemagglutinin Cleavage Site in H9 Avian Influenza Viruses Bioinformatics (Oxford, England). Aug, 2017 | Pubmed ID: 28383669 A Peptide-based Approach to Evaluate the Adaptability of Influenza A Virus to Humans Based on Its Hemagglutinin Proteolytic Cleavage Site PloS One. 2017 | Pubmed ID: 28358853 Safe Recombinant Outer Membrane Vesicles That Display M2e Elicit Heterologous Influenza Protection Molecular Therapy : the Journal of the American Society of Gene Therapy. 04, 2017 | Pubmed ID: 28215994 Murine Leukemia Virus (MLV)-based Coronavirus Spike-pseudotyped Particle Production and Infection Bio-protocol. Dec, 2016 | Pubmed ID: 28018942 A Camel-derived MERS-CoV with a Variant Spike Protein Cleavage Site and Distinct Fusion Activation Properties Emerging Microbes & Infections. Dec, 2016 | Pubmed ID: 27999426 Viral Fusion Efficacy of Specific H3N2 Influenza Virus Reassortant Combinations at Single-particle Level Scientific Reports. 10, 2016 | Pubmed ID: 27752100 Middle East Respiratory Syndrome Coronavirus Infection is Inhibited by Griffithsin Antiviral Research. 09, 2016 | Pubmed ID: 27424494 Fusion of Enveloped Viruses in Endosomes Traffic (Copenhagen, Denmark). 06, 2016 | Pubmed ID: 26935856 Recombinant M2e Outer Membrane Vesicle Vaccines Protect Against Lethal Influenza A Challenge in BALB/c Mice Vaccine. Mar, 2016 | Pubmed ID: 26827663 Human Transferrin Receptor Triggers an Alternative Tacaribe Virus Internalization Pathway Archives of Virology. Feb, 2016 | Pubmed ID: 26559962 Modification of the Hemagglutinin Cleavage Site Allows Indirect Activation of Avian Influenza Virus H9N2 by Bacterial Staphylokinase Virology. Aug, 2015 | Pubmed ID: 25841078 Host Cell Proteases: Critical Determinants of Coronavirus Tropism and Pathogenesis Virus Research. Apr, 2015 | Pubmed ID: 25445340 Variations in PH Sensitivity, Acid Stability, and Fusogenicity of Three Influenza Virus H3 Subtypes Journal of Virology. Jan, 2015 | Pubmed ID: 25320308 Host Cell Entry of Middle East Respiratory Syndrome Coronavirus After Two-step, Furin-mediated Activation of the Spike Protein Proceedings of the National Academy of Sciences of the United States of America. Oct, 2014 | Pubmed ID: 25288733 Genotypic Characterization of Canine Coronaviruses Associated with Fatal Canine Neonatal Enteritis in the United States Journal of Clinical Microbiology. Dec, 2014 | Pubmed ID: 25253797 Canine Enteric Coronaviruses: Emerging Viral Pathogens with Distinct Recombinant Spike Proteins Viruses. Aug, 2014 | Pubmed ID: 25153347 Inhibition of Influenza Virus Infection and Hemagglutinin Cleavage by the Protease Inhibitor HAI-2 Biochemical and Biophysical Research Communications. Jul, 2014 | Pubmed ID: 24978308 Influenza Entry Pathways in Polarized MDCK Cells Biochemical and Biophysical Research Communications. Jul, 2014 | Pubmed ID: 24878525 A Novel Activation Mechanism of Avian Influenza Virus H9N2 by Furin Journal of Virology. Feb, 2014 | Pubmed ID: 24257604 Utilization of Human DC-SIGN and L-SIGN for Entry and Infection of Host Cells by the New World Arenavirus, Junín Virus Biochemical and Biophysical Research Communications. Nov, 2013 | Pubmed ID: 24183720 Single Particle Assay of Coronavirus Membrane Fusion with Proteinaceous Receptor-embedded Supported Bilayers Biomaterials. Oct, 2013 | Pubmed ID: 23886734 Entry of Rhabdoviruses into Animal Cells Advances in Experimental Medicine and Biology. 2013 | Pubmed ID: 23884591 Entry of Influenza Virus Advances in Experimental Medicine and Biology. 2013 | Pubmed ID: 23884586 Mutation in Spike Protein Cleavage Site and Pathogenesis of Feline Coronavirus Emerging Infectious Diseases. Jul, 2013 | Pubmed ID: 23763835 Cleavage Activation of Human-adapted Influenza Virus Subtypes by Kallikrein-related Peptidases 5 and 12 The Journal of Biological Chemistry. Jun, 2013 | Pubmed ID: 23612974 Plasmin-mediated Activation of Pandemic H1N1 Influenza Virus Hemagglutinin is Independent of the Viral Neuraminidase Journal of Virology. May, 2013 | Pubmed ID: 23449787 Acquisition of a Novel Eleven Amino Acid Insertion Directly N-terminal to a Tetrabasic Cleavage Site Confers Intracellular Cleavage of an H7N7 Influenza Virus Hemagglutinin Virology. Dec, 2012 | Pubmed ID: 23051710 Influenza Virus-membrane Fusion Triggered by Proton Uncaging for Single Particle Studies of Fusion Kinetics Analytical Chemistry. Oct, 2012 | Pubmed ID: 22974237 Influenza Virus-mediated Membrane Fusion: Determinants of Hemagglutinin Fusogenic Activity and Experimental Approaches for Assessing Virus Fusion Viruses. 07, 2012 | Pubmed ID: 22852045 Mechanisms of Coronavirus Cell Entry Mediated by the Viral Spike Protein Viruses. 06, 2012 | Pubmed ID: 22816037 Cleavage Activation of the Human-adapted Influenza Virus Subtypes by Matriptase Reveals Both Subtype and Strain Specificities Journal of Virology. Oct, 2012 | Pubmed ID: 22811538 Characterization of a Recombinant Canine Coronavirus with a Distinct Receptor-binding (S1) Domain Virology. Sep, 2012 | Pubmed ID: 22609354 Expression of the C-type Lectins DC-SIGN or L-SIGN Alters Host Cell Susceptibility for the Avian Coronavirus, Infectious Bronchitis Virus Veterinary Microbiology. Jun, 2012 | Pubmed ID: 22340967 Promotion of Vesicular Stomatitis Virus Fusion by the Endosome-specific Phospholipid Bis(monoacylglycero)phosphate (BMP) FEBS Letters. Mar, 2011 | Pubmed ID: 21333650 Internalization and Fusion Mechanism of Vesicular Stomatitis Virus and Related Rhabdoviruses Future Virology. 2010 | Pubmed ID: 23516023 Modifications to the Hemagglutinin Cleavage Site Control the Virulence of a Neurotropic H1N1 Influenza Virus Journal of Virology. Sep, 2010 | Pubmed ID: 20554779 Elastase-mediated Activation of the Severe Acute Respiratory Syndrome Coronavirus Spike Protein at Discrete Sites Within the S2 Domain The Journal of Biological Chemistry. Jul, 2010 | Pubmed ID: 20507992 Feline Lectin Activity is Critical for the Cellular Entry of Feline Infectious Peritonitis Virus Journal of Virology. Aug, 2010 | Pubmed ID: 20484511 SARS-coronavirus Spike S2 Domain Flanked by Cysteine Residues C822 and C833 is Important for Activation of Membrane Fusion Virology. Oct, 2009 | Pubmed ID: 19717178 Characterization of a Highly Conserved Domain Within the Severe Acute Respiratory Syndrome Coronavirus Spike Protein S2 Domain with Characteristics of a Viral Fusion Peptide Journal of Virology. Aug, 2009 | Pubmed ID: 19439480 Activation of the SARS Coronavirus Spike Protein Via Sequential Proteolytic Cleavage at Two Distinct Sites Proceedings of the National Academy of Sciences of the United States of America. Apr, 2009 | Pubmed ID: 19321428 Activation of P38 MAPK by Feline Infectious Peritonitis Virus Regulates Pro-inflammatory Cytokine Production in Primary Blood-derived Feline Mononuclear Cells Virology. Feb, 2009 | Pubmed ID: 19058829 Utilization of DC-SIGN for Entry of Feline Coronaviruses into Host Cells Journal of Virology. Dec, 2008 | Pubmed ID: 18799586 Differential Role for Low PH and Cathepsin-mediated Cleavage of the Viral Spike Protein During Entry of Serotype II Feline Coronaviruses Veterinary Microbiology. Dec, 2008 | Pubmed ID: 18606506 Molecular Architecture of the Bipartite Fusion Loops of Vesicular Stomatitis Virus Glycoprotein G, a Class III Viral Fusion Protein The Journal of Biological Chemistry. Mar, 2008 | Pubmed ID: 18165228 Role of the Actin Cytoskeleton During Influenza Virus Internalization into Polarized Epithelial Cells Cellular Microbiology. Jul, 2007 | Pubmed ID: 17578407 Heparan Sulfate is a Selective Attachment Factor for the Avian Coronavirus Infectious Bronchitis Virus Beaudette Avian Diseases. Mar, 2007 | Pubmed ID: 17461266 Feline Aminopeptidase N is Not a Functional Receptor for Avian Infectious Bronchitis Virus Virology Journal. Feb, 2007 | Pubmed ID: 17324273 Avian Infectious Bronchitis Virus Enters Cells Via the Endocytic Pathway Advances in Experimental Medicine and Biology. 2006 | Pubmed ID: 17037550 Fluorescence Dequenching Assays of Coronavirus Fusion Advances in Experimental Medicine and Biology. 2006 | Pubmed ID: 17037536 Bioinformatic and Comparative Localization of Rab Proteins Reveals Functional Insights into the Uncharacterized GTPases Ypt10p and Ypt11p Molecular and Cellular Biology. Oct, 2006 | Pubmed ID: 16980630 The Avian Coronavirus Infectious Bronchitis Virus Undergoes Direct Low-pH-dependent Fusion Activation During Entry into Host Cells Journal of Virology. Apr, 2006 | Pubmed ID: 16537586 Role of Clathrin-mediated Endocytosis During Vesicular Stomatitis Virus Entry into Host Cells Virology. Jul, 2005 | Pubmed ID: 15936793 Influenza Virus Entry and Infection Require Host Cell N-linked Glycoprotein Proceedings of the National Academy of Sciences of the United States of America. Dec, 2004 | Pubmed ID: 15601777 The Ubiquitin-vacuolar Protein Sorting System is Selectively Required During Entry of Influenza Virus into Host Cells Traffic (Copenhagen, Denmark). Dec, 2003 | Pubmed ID: 14617349 Role for Influenza Virus Envelope Cholesterol in Virus Entry and Infection Journal of Virology. Dec, 2003 | Pubmed ID: 14610177 Rab GTPases Are Recruited to Chlamydial Inclusions in Both a Species-dependent and Species-independent Manner Infection and Immunity. Oct, 2003 | Pubmed ID: 14500507 Virus Nuclear Import Advanced Drug Delivery Reviews. Jun, 2003 | Pubmed ID: 12788537 Differential Requirements of Rab5 and Rab7 for Endocytosis of Influenza and Other Enveloped Viruses Traffic (Copenhagen, Denmark). May, 2003 | Pubmed ID: 12713661 Role of Protein Kinase C BetaII in Influenza Virus Entry Via Late Endosomes Journal of Virology. Jan, 2003 | Pubmed ID: 12477851 Influenza Virus Can Enter and Infect Cells in the Absence of Clathrin-mediated Endocytosis Journal of Virology. Oct, 2002 | Pubmed ID: 12239322 Dissecting Virus Entry Via Endocytosis The Journal of General Virology. Jul, 2002 | Pubmed ID: 12075072 Role of the Influenza Virus M1 Protein in Nuclear Export of Viral Ribonucleoproteins Journal of Virology. Feb, 2000 | Pubmed ID: 10644350 Production of Pseudotyped Particles to Study Highly Pathogenic Coronaviruses in a Biosafety Level 2 Setting Jean K. Millet1,2, Tiffany Tang3, Lakshmi Nathan3, Javier A. Jaimes4, Hung-Lun Hsu3,5, Susan Daniel3, Gary R. Whittaker1 1Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, 2INRA, Virologie et Immunologie Moléculaires, 3Robert Frederick Smith School of Chemical and Biomolecular Engineering, Cornell University, 4Department of Microbiology, College of Agricultural and Life Sciences, Cornell University, 5Horae Gene Therapy Center, University of Massachusetts Medical School JoVE 59010 Immunology and Infection A Fluorogenic Peptide Cleavage Assay to Screen for Proteolytic Activity: Applications for coronavirus spike protein activation Javier A. Jaimes*1,2, Jean K. Millet*1,3, Monty E. Goldstein1,4, Gary R. Whittaker1, Marco R. Straus1 1Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, 2Department of Microbiology, College of Agricultural and Life Sciences, Cornell University, 3Virologie et Immunologie Moléculaires, Domaine de Vilvert, INRA, 4Department of Cell Biology and Molecular Genetics, University of Maryland JoVE 58892 Biochemistry
Production of Pseudotyped Particles to Study Highly Pathogenic Coronaviruses in a Biosafety Level 2 Setting Jean K. Millet1,2, Tiffany Tang3, Lakshmi Nathan3, Javier A. Jaimes4, Hung-Lun Hsu3,5, Susan Daniel3, Gary R. Whittaker1 1Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, 2INRA, Virologie et Immunologie Moléculaires, 3Robert Frederick Smith School of Chemical and Biomolecular Engineering, Cornell University, 4Department of Microbiology, College of Agricultural and Life Sciences, Cornell University, 5Horae Gene Therapy Center, University of Massachusetts Medical School JoVE 59010 Immunology and Infection
A Fluorogenic Peptide Cleavage Assay to Screen for Proteolytic Activity: Applications for coronavirus spike protein activation Javier A. Jaimes*1,2, Jean K. Millet*1,3, Monty E. Goldstein1,4, Gary R. Whittaker1, Marco R. Straus1 1Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, 2Department of Microbiology, College of Agricultural and Life Sciences, Cornell University, 3Virologie et Immunologie Moléculaires, Domaine de Vilvert, INRA, 4Department of Cell Biology and Molecular Genetics, University of Maryland JoVE 58892 Biochemistry
