Articles by Geneva Stein in JoVE
C. elegans Positieve Butanon Learning, op korte termijn en lange termijn geheugen Associatieve Assays Amanda Kauffman1, Lance Parsons2, Geneva Stein1, Airon Wills1, Rachel Kaletsky1, Coleen Murphy1 1Department of Molecular Biology, Lewis-Sigler Institute for Integrative Genomics, Princeton University, 2Lewis-Sigler Institute for Integrative Genomics, Princeton University Hier beschrijven we methoden om te testen C. elegans associatief leren en korte-en lange termijn associatief geheugen. Deze populatie testen maken gebruik van de wormen mogelijkheden om chemotax in de richting van vluchtige geur-, en vormen positieve associaties bij de paring voedsel met de chemoattractant butanon. Verhoging van het aantal van conditionering periodes leidt tot lange-termijn geheugen.
Other articles by Geneva Stein on PubMed
Growth and Regeneration of Adult Beta Cells Does Not Involve Specialized Progenitors Developmental Cell. May, 2007 | Pubmed ID: 17488631 Cellular progenitors remain poorly characterized in many adult tissues, limited in part by the lack of unbiased techniques to identify progenitors and their progeny. To address this fundamental problem, we developed a novel DNA analog-based lineage-tracing technique to detect multiple rounds of cell division in vivo. Here, we apply this technique to determine the adult lineage mechanism of the insulin-secreting beta cells of pancreatic islets, an important unresolved question in diabetes research. As expected, gastrointestinal and skin epithelia involve specialized progenitors that repeatedly divide to give rise to postmitotic cells. In contrast, specialized progenitors do not contribute to adult beta cells, not even during acute beta cell regeneration. Instead, beta cells are the products of uniform self-renewal, slowed by a replication refractory period that prevents beta cells from immediately redividing. Our approach provides unbiased resolution of previously inaccessible developmental niches and can elucidate lineage mechanisms without candidate markers.
Establishment of Paternal Allele-specific DNA Methylation at the Imprinted Mouse Gtl2 Locus Epigenetics : Official Journal of the DNA Methylation Society. Aug, 2011 | Pubmed ID: 21725202 The monoallelic expression of imprinted genes is controlled by epigenetic factors including DNA methylation and histone modifications. In mouse, the imprinted gene Gtl2 is associated with two differentially methylated regions: the IG-DMR, which serves as a gametic imprinting mark at which paternal allele-specific DNA methylation is inherited from sperm, and the Gtl2-DMR, which acquires DNA methylation on the paternal allele after fertilization. The timeframe during which DNA methylation is acquired at secondary DMRs during post-fertilization development and the relationship between secondary DMRs and imprinted expression have not been well established. In order to better understand the role of secondary DMRs in imprinting, we examined the methylation status of the Gtl2-DMR in pre- and post-implantation embryos. Paternal allele-specific DNA methylation of this region correlates with imprinted expression of Gtl2 during post-implantation development but is not required to implement imprinted expression during pre-implantation development, suggesting that this secondary DMR may play a role in maintaining imprinted expression. Furthermore, our developmental profile of DNA methylation patterns at the Cdkn1c- and Gtl2-DMRs illustrates that the temporal acquisition of DNA methylation at imprinted genes during post-fertilization development is not universally controlled.