George M. Church Department of Genetics Harvard Medical School Biography Publications Institution JoVE Articles George M. Church has not added a biography. If you are George M. Church and would like to personalize this page please email our Author Liaison for assistance. Publications Predispositional Genome Sequencing in Healthy Adults: Design, Participant Characteristics, and Early Outcomes of the PeopleSeq Consortium Genome Medicine. Feb, 2019 | Pubmed ID: 30808425 REST and Neural Gene Network Dysregulation in IPSC Models of Alzheimer's Disease Cell Reports. Jan, 2019 | Pubmed ID: 30699343 Significant Abundance of Cis Configurations of Coding Variants in Diploid Human Genomes Nucleic Acids Research. Jan, 2019 | Pubmed ID: 30698752 Crystal Structure of the Redβ C-terminal Domain in Complex with λ Exonuclease Reveals an Unexpected Homology with λ Orf and an Interaction with Escherichia Coli Single Stranded DNA Binding Protein Nucleic Acids Research. Feb, 2019 | Pubmed ID: 30624736 CRISPR-C: Circularization of Genes and Chromosome by CRISPR in Human Cells Nucleic Acids Research. Dec, 2018 | Pubmed ID: 30551175 Characterizing Posttranslational Modifications in Prokaryotic Metabolism Using a Multiscale Workflow Proceedings of the National Academy of Sciences of the United States of America. 10, 2018 | Pubmed ID: 30301795 Establishing a Cell-Free Vibrio Natriegens Expression System ACS Synthetic Biology. Oct, 2018 | Pubmed ID: 30160938 Developmental Barcoding of Whole Mouse Via Homing CRISPR Science (New York, N.Y.). 08, 2018 | Pubmed ID: 30093604 Inter-homologue Repair in Fertilized Human Eggs? Nature. 08, 2018 | Pubmed ID: 30089924 Biosensor Libraries Harness Large Classes of Binding Domains for Construction of Allosteric Transcriptional Regulators Nature Communications. Aug, 2018 | Pubmed ID: 30082754 An Enhanced CRISPR Repressor for Targeted Mammalian Gene Regulation Nature Methods. Aug, 2018 | Pubmed ID: 30013045 Directed Differentiation of Human Induced Pluripotent Stem Cells into Mature Kidney Podocytes and Establishment of a Glomerulus Chip Nature Protocols. Jul, 2018 | Pubmed ID: 29995874 Enhanced Bacterial Immunity and Mammalian Genome Editing Via RNA-Polymerase-Mediated Dislodging of Cas9 from Double-Strand DNA Breaks Molecular Cell. Jul, 2018 | Pubmed ID: 29979968 Characterizing Protein Kinase Substrate Specificity Using the Proteomic Peptide Library (ProPeL) Approach Current Protocols in Chemical Biology. 06, 2018 | Pubmed ID: 29927115 Current CRISPR Gene Drive Systems Are Likely to Be Highly Invasive in Wild Populations ELife. Jun, 2018 | Pubmed ID: 29916367 High-throughput Creation and Functional Profiling of DNA Sequence Variant Libraries Using CRISPR-Cas9 in Yeast Nature Biotechnology. Jul, 2018 | Pubmed ID: 29786095 Codon Usage of Highly Expressed Genes Affects Proteome-wide Translation Efficiency Proceedings of the National Academy of Sciences of the United States of America. 05, 2018 | Pubmed ID: 29735666 High-resolution Mapping of DNA Polymerase Fidelity Using Nucleotide Imbalances and Next-generation Sequencing Nucleic Acids Research. Jul, 2018 | Pubmed ID: 29718339 Simultaneous Discovery of Cell-Free DNA and the Nucleosome Ladder Genetics. May, 2018 | Pubmed ID: 29716984 An Integrated Genome-wide CRISPRa Approach to Functionalize LncRNAs in Drug Resistance Cell. Apr, 2018 | Pubmed ID: 29677511 Enabling Multiplexed Testing of Pooled Donor Cells Through Whole-genome Sequencing Genome Medicine. 04, 2018 | Pubmed ID: 29673390 Response to "Unexpected Mutations After CRISPR-Cas9 Editing in Vivo" Nature Methods. Apr, 2018 | Pubmed ID: 29600989 Precise Cas9 Targeting Enables Genomic Mutation Prevention Proceedings of the National Academy of Sciences of the United States of America. 04, 2018 | Pubmed ID: 29555762 Adaptive Evolution of Genomically Recoded Proceedings of the National Academy of Sciences of the United States of America. 03, 2018 | Pubmed ID: 29440500 Spontaneous CRISPR Loci Generation in Vivo by Non-canonical Spacer Integration Nature Microbiology. Mar, 2018 | Pubmed ID: 29379209 From Designing the Molecules of Life to Designing Life: Future Applications Derived from Advances in DNA Technologies Angewandte Chemie (International Ed. in English). Apr, 2018 | Pubmed ID: 29316123 Engineering Posttranslational Proofreading to Discriminate Nonstandard Amino Acids Proceedings of the National Academy of Sciences of the United States of America. 01, 2018 | Pubmed ID: 29301968 The Future of Multiplexed Eukaryotic Genome Engineering ACS Chemical Biology. 02, 2018 | Pubmed ID: 29241002 Efficient in Situ Barcode Sequencing Using Padlock Probe-based BaristaSeq Nucleic Acids Research. Feb, 2018 | Pubmed ID: 29190363 Heterologous Erythromycin Production Across Strain and Plasmid Construction Biotechnology Progress. Jan, 2018 | Pubmed ID: 28960932 A CRISPR-Cas9-based Gene Drive Platform for Genetic Interaction Analysis in Candida Albicans Nature Microbiology. Oct, 2017 | Pubmed ID: 29062088 Mature Induced-pluripotent-stem-cell-derived Human Podocytes Reconstitute Kidney Glomerular-capillary-wall Function on a Chip Nature Biomedical Engineering. 2017 | Pubmed ID: 29038743 DNA Sequencing at 40: Past, Present and Future Nature. 10, 2017 | Pubmed ID: 29019985 Rapid Sequential in Situ Multiplexing with DNA Exchange Imaging in Neuronal Cells and Tissues Nano Letters. 10, 2017 | Pubmed ID: 28933153 A Systematic Comparison of Error Correction Enzymes by Next-generation Sequencing Nucleic Acids Research. Sep, 2017 | Pubmed ID: 28911123 Beyond Editing to Writing Large Genomes Nature Reviews. Genetics. 12, 2017 | Pubmed ID: 28852223 Imaging of Isolated Extracellular Vesicles Using Fluorescence Microscopy Methods in Molecular Biology (Clifton, N.J.). 2017 | Pubmed ID: 28828661 Extracellular Vesicle Isolation and Analysis by Western Blotting Methods in Molecular Biology (Clifton, N.J.). 2017 | Pubmed ID: 28828654 Inactivation of Porcine Endogenous Retrovirus in Pigs Using CRISPR-Cas9 Science (New York, N.Y.). 09, 2017 | Pubmed ID: 28798043 Rates, Distribution and Implications of Postzygotic Mosaic Mutations in Autism Spectrum Disorder Nature Neuroscience. 09, 2017 | Pubmed ID: 28714951 Dissecting Limiting Factors of the Protein Synthesis Using Recombinant Elements (PURE) System Translation (Austin, Tex.). 2017 | Pubmed ID: 28702280 CRISPR-Cas Encoding of a Digital Movie into the Genomes of a Population of Living Bacteria Nature. 07, 2017 | Pubmed ID: 28700573 The Promise of Organ and Tissue Preservation to Transform Medicine Nature Biotechnology. 06, 2017 | Pubmed ID: 28591112 Scalable Device for Automated Microbial Electroporation in a Digital Microfluidic Platform ACS Synthetic Biology. 09, 2017 | Pubmed ID: 28569062 Millstone: Software for Multiplex Microbial Genome Analysis and Engineering Genome Biology. 05, 2017 | Pubmed ID: 28545559 Optimizing Complex Phenotypes Through Model-guided Multiplex Genome Engineering Genome Biology. 05, 2017 | Pubmed ID: 28545477 Addressing the Ethical Issues Raised by Synthetic Human Entities with Embryo-like Features ELife. 03, 2017 | Pubmed ID: 28494856 Nucleotide-time Alignment for Molecular Recorders PLoS Computational Biology. May, 2017 | Pubmed ID: 28459860 Evolutionary Dynamics of CRISPR Gene Drives Science Advances. Apr, 2017 | Pubmed ID: 28435878 An Unbiased Index to Quantify Participant's Phenotypic Contribution to an Open-access Cohort Scientific Reports. 04, 2017 | Pubmed ID: 28387241 Cogenerating Synthetic Parts Toward a Self-Replicating System ACS Synthetic Biology. 07, 2017 | Pubmed ID: 28330337 Programmable Transcriptional Repression in Mycobacteria Using an Orthogonal CRISPR Interference Platform Nature Microbiology. Feb, 2017 | Pubmed ID: 28165460 SgRNA Scorer 2.0: A Species-Independent Model To Predict CRISPR/Cas9 Activity ACS Synthetic Biology. 05, 2017 | Pubmed ID: 28146356 Cushing's Syndrome Mutant PKA Exhibits Altered Substrate Specificity FEBS Letters. 02, 2017 | Pubmed ID: 28100013 Efficient, Footprint-free Human IPSC Genome Editing by Consolidation of Cas9/CRISPR and PiggyBac Technologies Nature Protocols. Jan, 2017 | Pubmed ID: 27929521 Rapidly Evolving Homing CRISPR Barcodes Nature Methods. 02, 2017 | Pubmed ID: 27918539 An Ethnically Relevant Consensus Korean Reference Genome is a Step Towards Personal Reference Genomes Nature Communications. 11, 2016 | Pubmed ID: 27882922 Complete Mitochondrial Genomes of Living and Extinct Pigeons Revise the Timing of the Columbiform Radiation BMC Evolutionary Biology. 10, 2016 | Pubmed ID: 27782796 Design and Characterization of a Nanopore-coupled Polymerase for Single-molecule DNA Sequencing by Synthesis on an Electrode Array Proceedings of the National Academy of Sciences of the United States of America. 11, 2016 | Pubmed ID: 27729524 The Whole Genome Sequences and Experimentally Phased Haplotypes of over 100 Personal Genomes GigaScience. Oct, 2016 | Pubmed ID: 27724973 Emergent Rules for Codon Choice Elucidated by Editing Rare Arginine Codons in Escherichia Coli Proceedings of the National Academy of Sciences of the United States of America. 09, 2016 | Pubmed ID: 27601680 A Multifunctional AAV-CRISPR-Cas9 and Its Host Response Nature Methods. 10, 2016 | Pubmed ID: 27595405 CRISPR-Cas9 System: Opportunities and Concerns Clinical Chemistry. 10, 2016 | Pubmed ID: 27551006 Design, Synthesis, and Testing Toward a 57-codon Genome Science (New York, N.Y.). Aug, 2016 | Pubmed ID: 27540174 Nanoscale Imaging of RNA with Expansion Microscopy Nature Methods. 08, 2016 | Pubmed ID: 27376770 Molecular Recordings by Directed CRISPR Spacer Acquisition Science (New York, N.Y.). Jul, 2016 | Pubmed ID: 27284167 Extensive Sequencing of Seven Human Genomes to Characterize Benchmark Reference Materials Scientific Data. Jun, 2016 | Pubmed ID: 27271295 Characterization of Cas9-Guide RNA Orthologs Cold Spring Harbor Protocols. 05, 2016 | Pubmed ID: 27140923 Characterizing Cas9 Protospacer-Adjacent Motifs with High-Throughput Sequencing of Library Depletion Experiments Cold Spring Harbor Protocols. 05, 2016 | Pubmed ID: 27140916 Real-time Single-molecule Electronic DNA Sequencing by Synthesis Using Polymer-tagged Nucleotides on a Nanopore Array Proceedings of the National Academy of Sciences of the United States of America. May, 2016 | Pubmed ID: 27091962 Performance of Optimized Noncanonical Amino Acid Mutagenesis Systems in the Absence of Release Factor 1 Molecular BioSystems. 05, 2016 | Pubmed ID: 27027374 Biosensor-based Engineering of Biosynthetic Pathways Current Opinion in Biotechnology. 12, 2016 | Pubmed ID: 26998575 Simulating Serial-Target Antibacterial Drug Synergies Using Flux Balance Analysis PloS One. 2016 | Pubmed ID: 26821252 In Vivo Gene Editing in Dystrophic Mouse Muscle and Muscle Stem Cells Science (New York, N.Y.). Jan, 2016 | Pubmed ID: 26721686 Engineering an Allosteric Transcription Factor to Respond to New Ligands Nature Methods. Feb, 2016 | Pubmed ID: 26689263 Efficient Reassignment of a Frequent Serine Codon in Wild-Type Escherichia Coli ACS Synthetic Biology. Feb, 2016 | Pubmed ID: 26544153 Precision Chemistry for Precision Medicine ACS Central Science. Mar, 2015 | Pubmed ID: 27162939 Crispr-mediated Gene Targeting of Human Induced Pluripotent Stem Cells Current Protocols in Stem Cell Biology. 2015 | Pubmed ID: 26949444 A General Strategy to Construct Small Molecule Biosensors in Eukaryotes ELife. Dec, 2015 | Pubmed ID: 26714111 Bioart Trends in Biotechnology. Dec, 2015 | Pubmed ID: 26617334 Safeguarding CRISPR-Cas9 Gene Drives in Yeast Nature Biotechnology. Dec, 2015 | Pubmed ID: 26571100 Neutralizing Antibodies Against West Nile Virus Identified Directly from Human B Cells by Single-cell Analysis and Next Generation Sequencing Integrative Biology : Quantitative Biosciences from Nano to Macro. Dec, 2015 | Pubmed ID: 26481611 A National Network of Neurotechnology Centers for the BRAIN Initiative Neuron. Nov, 2015 | Pubmed ID: 26481036 BIOSAFETY. Safeguarding Gene Drive Experiments in the Laboratory Science (New York, N.Y.). Aug, 2015 | Pubmed ID: 26229113 Puzzle Imaging: Using Large-Scale Dimensionality Reduction Algorithms for Localization PloS One. 2015 | Pubmed ID: 26192446 Unraveling CRISPR-Cas9 Genome Engineering Parameters Via a Library-on-library Approach Nature Methods. Sep, 2015 | Pubmed ID: 26167643 Synthetic Biosensors for Precise Gene Control and Real-time Monitoring of Metabolites Nucleic Acids Research. Sep, 2015 | Pubmed ID: 26152303 Genome and Transcriptome of Clostridium Phytofermentans, Catalyst for the Direct Conversion of Plant Feedstocks to Fuels PloS One. 2015 | Pubmed ID: 26035711 Highly Efficient Cas9-mediated Transcriptional Programming Nature Methods. Apr, 2015 | Pubmed ID: 25730490 Fluorescent in Situ Sequencing (FISSEQ) of RNA for Gene Expression Profiling in Intact Cells and Tissues Nature Protocols. Mar, 2015 | Pubmed ID: 25675209 Biocontainment of Genetically Modified Organisms by Synthetic Protein Design Nature. Feb, 2015 | Pubmed ID: 25607366 Insights into the Evolution of Longevity from the Bowhead Whale Genome Cell Reports. Jan, 2015 | Pubmed ID: 25565328 Complete Genome Sequences of T4-Like Bacteriophages RB3, RB5, RB6, RB7, RB9, RB10, RB27, RB33, RB55, RB59, and RB68 Genome Announcements. Jan, 2015 | Pubmed ID: 25555735 Accurate Whole Genome Sequencing As the Ultimate Genetic Test Clinical Chemistry. Jan, 2015 | Pubmed ID: 25479756 Multi-kilobase Homozygous Targeted Gene Replacement in Human Induced Pluripotent Stem Cells Nucleic Acids Research. Feb, 2015 | Pubmed ID: 25414332 Direct Mutagenesis of Thousands of Genomic Targets Using Microarray-derived Oligonucleotides ACS Synthetic Biology. Jan, 2015 | Pubmed ID: 24856730 Spatial Information in Large-scale Neural Recordings Frontiers in Computational Neuroscience. 2014 | Pubmed ID: 25653613 Evolution-guided Optimization of Biosynthetic Pathways Proceedings of the National Academy of Sciences of the United States of America. Dec, 2014 | Pubmed ID: 25453111 Multiple Haplotype-resolved Genomes Reveal Population Patterns of Gene and Protein Diplotypes Nature Communications. Nov, 2014 | Pubmed ID: 25424553 Rapid Neurogenesis Through Transcriptional Activation in Human Stem Cells Molecular Systems Biology. Nov, 2014 | Pubmed ID: 25403753 Genome Editing in Human Stem Cells Methods in Enzymology. 2014 | Pubmed ID: 25398338 Engineering Allostery Trends in Genetics : TIG. Dec, 2014 | Pubmed ID: 25306102 Multiplex Single-molecule Interaction Profiling of DNA-barcoded Proteins Nature. Nov, 2014 | Pubmed ID: 25252978 Improved Cell-free RNA and Protein Synthesis System PloS One. 2014 | Pubmed ID: 25180701 The Naked Mole Rat Genome Resource: Facilitating Analyses of Cancer and Longevity-related Adaptations Bioinformatics (Oxford, England). Dec, 2014 | Pubmed ID: 25172923 Concerning RNA-guided Gene Drives for the Alteration of Wild Populations ELife. 07, 2014 | Pubmed ID: 25035423 Genome Editing Assessment Using CRISPR Genome Analyzer (CRISPR-GA) Bioinformatics (Oxford, England). Oct, 2014 | Pubmed ID: 24990609 CRISPR/Cas9-Directed Genome Editing of Cultured Cells Current Protocols in Molecular Biology. Jul, 2014 | Pubmed ID: 24984853 CRISPR/Cas9-mediated Phage Resistance is Not Impeded by the DNA Modifications of Phage T4 PloS One. 2014 | Pubmed ID: 24886988 CHOPCHOP: a CRISPR/Cas9 and TALEN Web Tool for Genome Editing Nucleic Acids Research. Jul, 2014 | Pubmed ID: 24861617 Modeling the Mitochondrial Cardiomyopathy of Barth Syndrome with Induced Pluripotent Stem Cell and Heart-on-chip Technologies Nature Medicine. Jun, 2014 | Pubmed ID: 24813252 Fungal Lysis by a Soil Bacterium Fermenting Cellulose Environmental Microbiology. May, 2014 | Pubmed ID: 24798076 Large-scale De Novo DNA Synthesis: Technologies and Applications Nature Methods. May, 2014 | Pubmed ID: 24781323 Syntrophic Exchange in Synthetic Microbial Communities Proceedings of the National Academy of Sciences of the United States of America. May, 2014 | Pubmed ID: 24778240 Harvard Personal Genome Project: Lessons from Participatory Public Research Genome Medicine. Feb, 2014 | Pubmed ID: 24713084 High-resolution Antibody Dynamics of Vaccine-induced Immune Responses Proceedings of the National Academy of Sciences of the United States of America. Apr, 2014 | Pubmed ID: 24639495 Realizing the Potential of Synthetic Biology Nature Reviews. Molecular Cell Biology. 04, 2014 | Pubmed ID: 24622617 Raw Data: Access to Inaccuracy--response Science (New York, N.Y.). Feb, 2014 | Pubmed ID: 24578564 Highly Multiplexed Subcellular RNA Sequencing in Situ Science (New York, N.Y.). Mar, 2014 | Pubmed ID: 24578530 Recoding the Genetic Code with Selenocysteine Angewandte Chemie (International Ed. in English). Jan, 2014 | Pubmed ID: 24511637 Information Access. Raw Personal Data: Providing Access Science (New York, N.Y.). Jan, 2014 | Pubmed ID: 24458627 Rational Optimization of TolC As a Powerful Dual Selectable Marker for Genome Engineering Nucleic Acids Research. Apr, 2014 | Pubmed ID: 24452804 Integrating B Cell Lineage Information into Statistical Tests for Detecting Selection in Ig Sequences Journal of Immunology (Baltimore, Md. : 1950). Feb, 2014 | Pubmed ID: 24376267 The Role of Replicates for Error Mitigation in Next-generation Sequencing Nature Reviews. Genetics. 01, 2014 | Pubmed ID: 24322726 Natural Reassignment of CUU and CUA Sense Codons to Alanine in Ashbya Mitochondria Nucleic Acids Research. Jan, 2014 | Pubmed ID: 24049072 Next-generation Carrier Screening Genetics in Medicine : Official Journal of the American College of Medical Genetics. Feb, 2014 | Pubmed ID: 23765052 The Effects of Somatic Hypermutation on Neutralization and Binding in the PGT121 Family of Broadly Neutralizing HIV Antibodies PLoS Pathogens. 2013 | Pubmed ID: 24278016 BRAIN: Innovative Neurotechnologies for Imaging and Therapeutics Dialogues in Clinical Neuroscience. Sep, 2013 | Pubmed ID: 24174897 Deciphering the Functions and Regulation of Brain-enriched A-to-I RNA Editing Nature Neuroscience. Nov, 2013 | Pubmed ID: 24165678 Yeast Oligo-mediated Genome Engineering (YOGE) ACS Synthetic Biology. Dec, 2013 | Pubmed ID: 24160921 Genomically Recoded Organisms Expand Biological Functions Science (New York, N.Y.). Oct, 2013 | Pubmed ID: 24136966 Cas9 As a Versatile Tool for Engineering Biology Nature Methods. Oct, 2013 | Pubmed ID: 24076990 Orthogonal Cas9 Proteins for RNA-guided Gene Regulation and Editing Nature Methods. Nov, 2013 | Pubmed ID: 24076762 Mutants of Cre Recombinase with Improved Accuracy Nature Communications. 2013 | Pubmed ID: 24056590 Heritable Custom Genomic Modifications in Caenorhabditis Elegans Via a CRISPR-Cas9 System Genetics. Nov, 2013 | Pubmed ID: 23979579 Multiplex and Homologous Recombination-mediated Genome Editing in Arabidopsis and Nicotiana Benthamiana Using Guide RNA and Cas9 Nature Biotechnology. Aug, 2013 | Pubmed ID: 23929339 Composability of Regulatory Sequences Controlling Transcription and Translation in Escherichia Coli Proceedings of the National Academy of Sciences of the United States of America. Aug, 2013 | Pubmed ID: 23924614 CAS9 Transcriptional Activators for Target Specificity Screening and Paired Nickases for Cooperative Genome Engineering Nature Biotechnology. Sep, 2013 | Pubmed ID: 23907171 Optical Control of Mammalian Endogenous Transcription and Epigenetic States Nature. Aug, 2013 | Pubmed ID: 23877069 Quantification of MicroRNA Expression with Next-generation Sequencing Current Protocols in Molecular Biology. Jul, 2013 | Pubmed ID: 23821442 Heritable Genome Editing in C. Elegans Via a CRISPR-Cas9 System Nature Methods. Aug, 2013 | Pubmed ID: 23817069 In Vitro Integration of Ribosomal RNA Synthesis, Ribosome Assembly, and Translation Molecular Systems Biology. Jun, 2013 | Pubmed ID: 23799452 Nanotools for Neuroscience and Brain Activity Mapping ACS Nano. Mar, 2013 | Pubmed ID: 23514423 Barcoding Cells Using Cell-surface Programmable DNA-binding Domains Nature Methods. May, 2013 | Pubmed ID: 23503053 PhIP-Seq Characterization of Autoantibodies from Patients with Multiple Sclerosis, Type 1 Diabetes and Rheumatoid Arthritis Journal of Autoimmunity. Jun, 2013 | Pubmed ID: 23497938 Neuroscience. The Brain Activity Map Science (New York, N.Y.). Mar, 2013 | Pubmed ID: 23470729 Genome Engineering in Saccharomyces Cerevisiae Using CRISPR-Cas Systems Nucleic Acids Research. Apr, 2013 | Pubmed ID: 23460208 Redirector: Designing Cell Factories by Reconstructing the Metabolic Objective PLoS Computational Biology. 2013 | Pubmed ID: 23341769 Reading and Writing Omes Molecular Systems Biology. 2013 | Pubmed ID: 23340848 An Analysis and Validation Pipeline for Large-scale RNAi-based Screens Scientific Reports. 2013 | Pubmed ID: 23326633 RNA-guided Human Genome Engineering Via Cas9 Science (New York, N.Y.). Feb, 2013 | Pubmed ID: 23287722 Gene Assembly from Chip-Synthesized Oligonucleotides Current Protocols in Chemical Biology. Mar, 2012 | Pubmed ID: 25077042 Using Bacteria to Determine Protein Kinase Specificity and Predict Target Substrates PloS One. 2012 | Pubmed ID: 23300758 Submicrometre Geometrically Encoded Fluorescent Barcodes Self-assembled from DNA Nature Chemistry. Oct, 2012 | Pubmed ID: 23000997 Improving Lambda Red Genome Engineering in Escherichia Coli Via Rational Removal of Endogenous Nucleases PloS One. 2012 | Pubmed ID: 22957093 Next-generation Digital Information Storage in DNA Science (New York, N.Y.). Sep, 2012 | Pubmed ID: 22903519 How Accurate Can Genetic Predictions Be? BMC Genomics. Jul, 2012 | Pubmed ID: 22827772 Un Recurso Público, Facilitando El Uso Clínico De Los Genomas Proceedings of the National Academy of Sciences of the United States of America. Jul, 2012 | Pubmed ID: 22797899 Accurate Whole-genome Sequencing and Haplotyping from 10 to 20 Human Cells Nature. Jul, 2012 | Pubmed ID: 22785314 Iterative Capped Assembly: Rapid and Scalable Synthesis of Repeat-module DNA Such As TAL Effectors from Individual Monomers Nucleic Acids Research. Aug, 2012 | Pubmed ID: 22740649 Multiplexed in Vivo His-tagging of Enzyme Pathways for in Vitro Single-pot Multienzyme Catalysis ACS Synthetic Biology. Feb, 2012 | Pubmed ID: 22737598 The Brain Activity Map Project and the Challenge of Functional Connectomics Neuron. Jun, 2012 | Pubmed ID: 22726828 A BioBrick Compatible Strategy for Genetic Modification of Plants Journal of Biological Engineering. 2012 | Pubmed ID: 22716313 Enhanced Multiplex Genome Engineering Through Co-operative Oligonucleotide Co-selection Nucleic Acids Research. Sep, 2012 | Pubmed ID: 22638574 Stable Gene Targeting in Human Cells Using Single-strand Oligonucleotides with Modified Bases PloS One. 2012 | Pubmed ID: 22615794 Systematic Identification of Edited MicroRNAs in the Human Brain Genome Research. Aug, 2012 | Pubmed ID: 22499667 Genome-scale Promoter Engineering by Coselection MAGE Nature Methods. Jun, 2012 | Pubmed ID: 22484848 High-throughput Multiplex Sequencing of MiRNA Current Protocols in Human Genetics. Apr, 2012 | Pubmed ID: 22470142 Back to the Future: from Genome to Metabolome Human Mutation. May, 2012 | Pubmed ID: 22431014 Genomic Analysis of the Hydrocarbon-producing, Cellulolytic, Endophytic Fungus Ascocoryne Sarcoides PLoS Genetics. 2012 | Pubmed ID: 22396667 A Logic-gated Nanorobot for Targeted Transport of Molecular Payloads Science (New York, N.Y.). Feb, 2012 | Pubmed ID: 22344439 La Secuencia De RNA Revela La Expresión Diferencial De Mitocondrial Y Genes De Reducción De Oxidación En La Larga Vida Desnuda Mole-rata En Comparación Con Ratones PloS One. 2011 | Pubmed ID: 22073188 La Arquitectura Tridimensional De Un Genoma Bacteriano Y Su Alteración Por La Perturbación Genética Molecular Cell. Oct, 2011 | Pubmed ID: 22017872 Panel Discussion: Can There Be a Biomarker for Sleepiness? Journal of Clinical Sleep Medicine : JCSM : Official Publication of the American Academy of Sleep Medicine. Oct, 2011 | Pubmed ID: 22003333 New Technologies for Integrating Genomic, Environmental and Trait Data Journal of Clinical Sleep Medicine : JCSM : Official Publication of the American Academy of Sleep Medicine. Oct, 2011 | Pubmed ID: 22003332 Phased Whole-genome Genetic Risk in a Family Quartet Using a Major Allele Reference Sequence PLoS Genetics. Sep, 2011 | Pubmed ID: 21935354 Precise Manipulation of Chromosomes in Vivo Enables Genome-wide Codon Replacement Science (New York, N.Y.). Jul, 2011 | Pubmed ID: 21764749 Barcoding Bias in High-throughput Multiplex Sequencing of MiRNA Genome Research. Sep, 2011 | Pubmed ID: 21750102 Multiplexed Genome Engineering and Genotyping Methods Applications for Synthetic Biology and Metabolic Engineering Methods in Enzymology. 2011 | Pubmed ID: 21601688 Proteome-wide Systems Analysis of a Cellulosic Biofuel-producing Microbe Molecular Systems Biology. Jan, 2011 | Pubmed ID: 21245846 Research, Collaboration, and Open Science Using Web 2.0 Journal of Microbiology & Biology Education. 2010 | Pubmed ID: 23653712 The Human Microbiome Harbors a Diverse Reservoir of Antibiotic Resistance Genes Virulence. Jul-Aug, 2010 | Pubmed ID: 21178459 Scalable Gene Synthesis by Selective Amplification of DNA Pools from High-fidelity Microchips Nature Biotechnology. Dec, 2010 | Pubmed ID: 21113165 Collection and Motif-based Prediction of Phosphorylation Sites in Human Viruses Science Signaling. Aug, 2010 | Pubmed ID: 20807955 La Reprogramación De Las Células T De Sangre Periférica Humana Cell Stem Cell. Jul, 2010 | Pubmed ID: 20621044 A Survey of Genomic Traces Reveals a Common Sequencing Error, RNA Editing, and DNA Editing PLoS Genetics. May, 2010 | Pubmed ID: 20531933 A Functional Metagenomic Approach for Expanding the Synthetic Biology Toolbox for Biomass Conversion Molecular Systems Biology. Apr, 2010 | Pubmed ID: 20393580 Extensive Phosphorylation with Overlapping Specificity by Mycobacterium Tuberculosis Serine/threonine Protein Kinases Proceedings of the National Academy of Sciences of the United States of America. Apr, 2010 | Pubmed ID: 20368441 Generación De Endodermo Funcional Hepático Humano a Partir De Células Madre Pluripotentes Inducidas Hepatology (Baltimore, Md.). Jan, 2010 | Pubmed ID: 19877180 Un Enfoque Sólido Para Identificación De Tejidos Específicos De Expresión Génica Utilizando Variantes De Regulación Personalizados Humanos Células Madre Pluripotentes Inducidas PLoS Genetics. Nov, 2009 | Pubmed ID: 19911041 Targeted Gene Inactivation in Clostridium Phytofermentans Shows That Cellulose Degradation Requires the Family 9 Hydrolase Cphy3367 Molecular Microbiology. Dec, 2009 | Pubmed ID: 19775243 Evidence for Large Diversity in the Human Transcriptome Created by Alu RNA Editing Nucleic Acids Research. Nov, 2009 | Pubmed ID: 19740767 Functional Characterization of the Antibiotic Resistance Reservoir in the Human Microflora Science (New York, N.Y.). Aug, 2009 | Pubmed ID: 19713526 Large-scale Identification of Genetic Design Strategies Using Local Search Molecular Systems Biology. 2009 | Pubmed ID: 19690565 Programming Cells by Multiplex Genome Engineering and Accelerated Evolution Nature. Aug, 2009 | Pubmed ID: 19633652 The Scientific Foundation for Personal Genomics: Recommendations from a National Institutes of Health-Centers for Disease Control and Prevention Multidisciplinary Workshop Genetics in Medicine : Official Journal of the American College of Medical Genetics. Aug, 2009 | Pubmed ID: 19617843 A Highly Annotated Whole-genome Sequence of a Korean Individual Nature. Aug, 2009 | Pubmed ID: 19587683 Rapid Prototyping of 3D DNA-origami Shapes with CaDNAno Nucleic Acids Research. Aug, 2009 | Pubmed ID: 19531737 Multiplex Padlock Targeted Sequencing Reveals Human Hypermutable CpG Variations Genome Research. Sep, 2009 | Pubmed ID: 19525355 Genome-wide Identification of Human RNA Editing Sites by Parallel DNA Capturing and Sequencing Science (New York, N.Y.). May, 2009 | Pubmed ID: 19478186 Dirigido a Escala Del Genoma Y Las Estrategias Revelar El Cuerpo Gene-Firmas De Metilación En Las Células Humanas Nature Biotechnology. Apr, 2009 | Pubmed ID: 19329998 Meta-analysis of Age-related Gene Expression Profiles Identifies Common Signatures of Aging Bioinformatics (Oxford, England). Apr, 2009 | Pubmed ID: 19189975 The Human Ageing Genomic Resources: Online Databases and Tools for Biogerontologists Aging Cell. Feb, 2009 | Pubmed ID: 18986374 Predicting Protein Post-translational Modifications Using Meta-analysis of Proteome Scale Data Sets Molecular & Cellular Proteomics : MCP. Feb, 2009 | Pubmed ID: 18974045 Efficient MicroRNA Capture and Bar-coding Via Enzymatic Oligonucleotide Adenylation Nature Methods. Sep, 2008 | Pubmed ID: 19160512 Model-driven Analysis of Experimentally Determined Growth Phenotypes for 465 Yeast Gene Deletion Mutants Under 16 Different Conditions Genome Biology. 2008 | Pubmed ID: 18808699 Hippocrates Revisited? Old Ideals and New Realities Genomic Medicine. Jan, 2008 | Pubmed ID: 18716915 Bacteria Subsisting on Antibiotics Science (New York, N.Y.). Apr, 2008 | Pubmed ID: 18388292 Gene Synthesis by Circular Assembly Amplification Nature Methods. Jan, 2008 | Pubmed ID: 18037891 Patterns and Implications of Gene Gain and Loss in the Evolution of Prochlorococcus PLoS Genetics. Dec, 2007 | Pubmed ID: 18159947 Multiplex Amplification of Large Sets of Human Exons Nature Methods. Nov, 2007 | Pubmed ID: 17934468 A Proposal to Sequence Genomes of Unique Interest for Research on Aging The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences. Jun, 2007 | Pubmed ID: 17595413 Chromosomal Periodicity of Evolutionarily Conserved Gene Pairs Proceedings of the National Academy of Sciences of the United States of America. Jun, 2007 | Pubmed ID: 17563360 Analyses of Human-chimpanzee Orthologous Gene Pairs to Explore Evolutionary Hypotheses of Aging Mechanisms of Ageing and Development. May-Jun, 2007 | Pubmed ID: 17459455 An Analysis of the Relationship Between Metabolism, Developmental Schedules, and Longevity Using Phylogenetic Independent Contrasts The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences. Feb, 2007 | Pubmed ID: 17339640 Polony DNA Sequencing Current Protocols in Molecular Biology. Nov, 2006 | Pubmed ID: 18265387 Relationships Between P63 Binding, DNA Sequence, Transcription Activity, and Biological Function in Human Cells Molecular Cell. Nov, 2006 | Pubmed ID: 17188034 The Transition Between Transcriptional Initiation and Elongation in E. Coli is Highly Variable and Often Rate Limiting Molecular Cell. Dec, 2006 | Pubmed ID: 17157257 Global Gene Expression of Prochlorococcus Ecotypes in Response to Changes in Nitrogen Availability Molecular Systems Biology. 2006 | Pubmed ID: 17016519 Mamíferos Ultraconserved Elementos Fuertemente Se Agotan Entre Duplicaciones Segmentarias Y Copiar Números Variantes Nature Genetics. Oct, 2006 | Pubmed ID: 16998490 Identifying Regulatory Mechanisms Using Individual Variation Reveals Key Role for Chromatin Modification Proceedings of the National Academy of Sciences of the United States of America. Sep, 2006 | Pubmed ID: 16968785 Towards Synthesis of a Minimal Cell Molecular Systems Biology. 2006 | Pubmed ID: 16924266 Sequencing Genomes from Single Cells by Polymerase Cloning Nature Biotechnology. Jun, 2006 | Pubmed ID: 16732271 Identifying Metabolic Enzymes with Multiple Types of Association Evidence BMC Bioinformatics. Mar, 2006 | Pubmed ID: 16571130 Long-range Polony Haplotyping of Individual Human Chromosome Molecules Nature Genetics. Mar, 2006 | Pubmed ID: 16493423 An Integrated Strategy for Analyzing the Unique Developmental Programs of Different Myoblast Subtypes PLoS Genetics. Feb, 2006 | Pubmed ID: 16482229 MapQuant: Open-source Software for Large-scale Protein Quantification Proteomics. Mar, 2006 | Pubmed ID: 16470651 Genomes for All Scientific American. Jan, 2006 | Pubmed ID: 16468433 Cells Discover Fire: Employing Reactive Oxygen Species in Development and Consequences for Aging Experimental Gerontology. Jan, 2006 | Pubmed ID: 16226003 From Systems Biology to Synthetic Biology Molecular Systems Biology. 2005 | Pubmed ID: 16729067 Expression Dynamics of a Cellular Metabolic Network Molecular Systems Biology. 2005 | Pubmed ID: 16729051 Genomic Analysis of LexA Binding Reveals the Permissive Nature of the Escherichia Coli Genome and Identifies Unconventional Target Sites Genes & Development. Nov, 2005 | Pubmed ID: 16264194 A Network of Transcriptionally Coordinated Functional Modules in Saccharomyces Cerevisiae Genome Research. Sep, 2005 | Pubmed ID: 16109970 Accurate Multiplex Polony Sequencing of an Evolved Bacterial Genome Science (New York, N.Y.). Sep, 2005 | Pubmed ID: 16081699 Genomes Optimize Reproduction: Aging As a Consequence of the Developmental Program Physiology (Bethesda, Md.). Aug, 2005 | Pubmed ID: 16024513 Molecular Weight Assessment of Proteins in Total Proteome Profiles Using 1D-PAGE and LC/MS/MS Proteome Science. Jun, 2005 | Pubmed ID: 15941491 Discovering Functional Transcription-factor Combinations in the Human Cell Cycle Genome Research. Jun, 2005 | Pubmed ID: 15930495 Preferred Analysis Methods for Affymetrix GeneChips Revealed by a Wholly Defined Control Dataset Genome Biology. 2005 | Pubmed ID: 15693945 Modular Epistasis in Yeast Metabolism Nature Genetics. Jan, 2005 | Pubmed ID: 15592468 Estimating and Improving Protein Interaction Error Rates Proceedings. IEEE Computational Systems Bioinformatics Conference. 2004 | Pubmed ID: 16448015 The Complete Genome and Proteome of Mycoplasma Mobile Genome Research. Aug, 2004 | Pubmed ID: 15289470 Filling Gaps in a Metabolic Network Using Expression Information Bioinformatics (Oxford, England). Aug, 2004 | Pubmed ID: 15262797 Preferente En Los Sitios Del Ubiquitinación Vivo Bioinformatics (Oxford, England). Dec, 2004 | Pubmed ID: 15256413 Predicción De Actuar Del Mismo Modo Módulos Cis-regulación Por Subsequence Genómica Comparativa Y Perfilado En Drosophila Melanogaster Y D.pseudoobscura Bioinformatics (Oxford, England). Nov, 2004 | Pubmed ID: 15145800 Advanced Sequencing Technologies: Methods and Goals Nature Reviews. Genetics. May, 2004 | Pubmed ID: 15143316 Mathematical Models of Diffusion-constrained Polymerase Chain Reactions: Basis of High-throughput Nucleic Acid Assays and Simple Self-organizing Systems Journal of Theoretical Biology. May, 2004 | Pubmed ID: 15064081 A Motif Co-occurrence Approach for Genome-wide Prediction of Transcription-factor-binding Sites in Escherichia Coli Genome Research. Feb, 2004 | Pubmed ID: 14762058 Identification of Many MicroRNAs That Copurify with Polyribosomes in Mammalian Neurons Proceedings of the National Academy of Sciences of the United States of America. Jan, 2004 | Pubmed ID: 14691248 YMGV: a Cross-species Expression Data Mining Tool Nucleic Acids Research. Jan, 2004 | Pubmed ID: 14681424 The Amino-acid Mutational Spectrum of Human Genetic Disease Genome Biology. 2003 | Pubmed ID: 14611658 Parallel Competition Analysis of Saccharomyces Cerevisiae Strains Differing by a Single Base Using Polymerase Colonies Nucleic Acids Research. Aug, 2003 | Pubmed ID: 12888536 Digital Genotyping and Haplotyping with Polymerase Colonies Proceedings of the National Academy of Sciences of the United States of America. May, 2003 | Pubmed ID: 12730373 Global RNA Half-life Analysis in Escherichia Coli Reveals Positional Patterns of Transcript Degradation Genome Research. Feb, 2003 | Pubmed ID: 12566399 A Microarray-based Antibiotic Screen Identifies a Regulatory Role for Supercoiling in the Osmotic Stress Response of Escherichia Coli Genome Research. Feb, 2003 | Pubmed ID: 12566398 Genome-wide Co-occurrence of Promoter Elements Reveals a Cis-regulatory Cassette of RRNA Transcription Motifs in Saccharomyces Cerevisiae Genome Research. Nov, 2002 | Pubmed ID: 12421759 Analysis of Optimality in Natural and Perturbed Metabolic Networks Proceedings of the National Academy of Sciences of the United States of America. Nov, 2002 | Pubmed ID: 12415116 Escherichia Coli Gene Expression Responsive to Levels of the Response Regulator EvgA Journal of Bacteriology. Nov, 2002 | Pubmed ID: 12399493 Computational Discovery of Sense-antisense Transcription in the Human and Mouse Genomes Genome Biology. Aug, 2002 | Pubmed ID: 12225583 Genome-scale Metabolic Model of Helicobacter Pylori 26695 Journal of Bacteriology. Aug, 2002 | Pubmed ID: 12142428 Computation-based Discovery of Related Transcriptional Regulatory Modules and Motifs Using an Experimentally Validated Combinatorial Model Genome Research. Jul, 2002 | Pubmed ID: 12097338 Measuring Absolute Expression with Microarrays with a Calibrated Reference Sample and an Extended Signal Intensity Range Proceedings of the National Academy of Sciences of the United States of America. May, 2002 | Pubmed ID: 12032321 Discrimination Between Paralogs Using Microarray Analysis: Application to the Yap1p and Yap2p Transcriptional Networks Molecular Biology of the Cell. May, 2002 | Pubmed ID: 12006656 Nucleotides of Transcription Factor Binding Sites Exert Interdependent Effects on the Binding Affinities of Transcription Factors Nucleic Acids Research. Mar, 2002 | Pubmed ID: 11861919 Exploring the DNA-binding Specificities of Zinc Fingers with DNA Microarrays Proceedings of the National Academy of Sciences of the United States of America. Jun, 2001 | Pubmed ID: 11404456 Predicting Regulons and Their Cis-regulatory Motifs by Comparative Genomics Nucleic Acids Research. Nov, 2000 | Pubmed ID: 11071941 Sin células proteína expresión usando el crecimiento rápido de bacteria Vibrio natriegens Daniel J. Wiegand1,2, Henry H. Lee1, Nili Ostrov*1, George M. Church*1,2 1Department of Genetics, Harvard Medical School, 2Wyss Institute for Biologically Inspired Engineering JoVE 59495 Bioengineering
Sin células proteína expresión usando el crecimiento rápido de bacteria Vibrio natriegens Daniel J. Wiegand1,2, Henry H. Lee1, Nili Ostrov*1, George M. Church*1,2 1Department of Genetics, Harvard Medical School, 2Wyss Institute for Biologically Inspired Engineering JoVE 59495 Bioengineering