In JoVE (1)
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Articles by Hamida Merzougui in JoVE
Nuclear Magnetic Resonance Spectroscopy for the Identification of Multiple Phosphorylations of Intrinsically Disordered Proteins Clément Danis*1,2, Clément Despres*1, Luiza M. Bessa1, Idir Malki1, Hamida Merzougui1, Isabelle Huvent1, Haoling Qi1, Guy Lippens1, François-Xavier Cantrelle1, Robert Schneider1, Xavier Hanoulle1, Caroline Smet-Nocca1, Isabelle Landrieu1 1UMR8576, CNRS, Lille University, 2UMR-S1172, INSERM CNRS, Lille University We describe here a method to identify multiple phosphorylations of an intrinsically disordered protein by Nuclear Magnetic Resonance Spectroscopy (NMR), using Tau protein as a case study. Recombinant Tau is isotopically enriched and modified in vitro by a kinase prior to data acquisition and analysis.