Other Publications (1)
Articles by Hannah Grover in JoVE
Probing the Roles of Physical Forces in Early Chick Embryonic Morphogenesis Yan Li*1, Hannah Grover*1, Eric Dai2, Kevin Yang1, Zi Chen1 1Thayer School of Engineering, Dartmouth College, 2Department of Bioengineering, University of Pennsylvania Here, we present a protocol introducing a set of new ex-ovo experiments and physical modeling approaches for studying the mechanics of morphogenesis during early chick embryonic brain torsion.
Other articles by Hannah Grover on PubMed
Modeling Physiological Events in 2D Vs. 3D Cell Culture Physiology (Bethesda, Md.). | Pubmed ID: 28615311 Cell culture has become an indispensable tool to help uncover fundamental biophysical and biomolecular mechanisms by which cells assemble into tissues and organs, how these tissues function, and how that function becomes disrupted in disease. Cell culture is now widely used in biomedical research, tissue engineering, regenerative medicine, and industrial practices. Although flat, two-dimensional (2D) cell culture has predominated, recent research has shifted toward culture using three-dimensional (3D) structures, and more realistic biochemical and biomechanical microenvironments. Nevertheless, in 3D cell culture, many challenges remain, including the tissue-tissue interface, the mechanical microenvironment, and the spatiotemporal distributions of oxygen, nutrients, and metabolic wastes. Here, we review 2D and 3D cell culture methods, discuss advantages and limitations of these techniques in modeling physiologically and pathologically relevant processes, and suggest directions for future research.