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Articles by Henrique S. Seckler in JoVE
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CN-GELFrEE - Clear Native Gel-eluted Liquid Fraction Entrapment Electrophoresis
Rafael D. Melani*1,2, Henrique S. Seckler*1, Owen S. Skinner1, Luis H. F. Do Vale1,3, Adam D. Catherman1, Pierre C. Havugimana1, Marcelo Valle de Sousa3, Gilberto B. Domont2, Neil L. Kelleher1, Philip D. Compton1
1Departments of Chemistry and Molecular Biosciences, Chemistry of Life Processes Institute, Proteomics Center of Excellence, Robert H. Lurie Comprehensive Cancer Center, Northwestern University, 2Institute of Chemistry, Proteomics Unit, Federal University of Rio de Janeiro, 3Department of Cell Biology, Brazilian Center for Protein Research, Laboratory of Biochemistry and Protein Chemistry, University of Brasilia
This protocol describes how to prepare and perform clear native gel-eluted liquid fraction entrapment electrophoresis (CN-GELFrEE), a native separation technique for non-covalent biomolecular assemblies and proteins from heterogeneous samples that is compatible with various downstream protein analysis techniques.
Other articles by Henrique S. Seckler on PubMed
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