Hiroshi Kitani Transgenic Animal Research Center National Institute of Agrobiological Sciences, Tsukuba, Japan Biography Publications Institution JoVE Articles Hiroshi Kitani has not added a biography. If you are Hiroshi Kitani and would like to personalize this page please email our Author Liaison for assistance. Publications Critical Roles of the WASP N-Terminal Domain and Btk in LPS-Induced Inflammatory Response in Macrophages PloS One. 2012 | Pubmed ID: 22253930 Carnosic Acid, a Pro-electrophilic Compound, Inhibits LPS-induced Activation of Microglia Biochemical and Biophysical Research Communications. Feb, 2012 | Pubmed ID: 22214931 PLD$ is Involved in Phagocytosis of Microglia: Expression and Localization Changes of PLD4 Are Correlated with Activation State of Microglia PloS One. 2011 | Pubmed ID: 22102906 Identification of Fyn As the Binding Partner for the WASP N-terminal Domain in T Cells International Immunology. Aug, 2011 | Pubmed ID: 21705469 The Activation of P2X7 Receptor Induces Cathepsin D-dependent Production of a 20-kDa Form of IL-1β Under Acidic Extracellular PH in LPS-primed Microglial Cells Journal of Neurochemistry. May, 2011 | Pubmed ID: 21395581 Isolation and Characterization of Macrophages from a Mixed Primary Culture of Bovine Liver Cells Veterinary Immunology and Immunopathology. Apr, 2011 | Pubmed ID: 21334751 Angiopoietin-1/Tie2 Signal Augments Basal Notch Signal Controlling Vascular Quiescence by Inducing Delta-like 4 Expression Through AKT-mediated Activation of Beta-catenin The Journal of Biological Chemistry. Mar, 2011 | Pubmed ID: 21212269 Cold Stress Induced Morphological Microglial Activation and Increased IL-1β Expression in Astroglial Cells in Rat Brain Journal of Neuroimmunology. Apr, 2011 | Pubmed ID: 21115202 Enhancement of Phagocytotic Activity by Prion Protein in PrP-deficient Macrophage Cells International Journal of Molecular Medicine. Oct, 2010 | Pubmed ID: 20818492 A Novel Isolation Method for Macrophage-like Cells from Mixed Primary Cultures of Adult Rat Liver Cells Journal of Immunological Methods. Aug, 2010 | Pubmed ID: 20600081 Celastrol Suppresses Morphological and Transcriptional Responses in Microglial Cells Upon Stimulation with Double-stranded RNA The International Journal of Neuroscience. Apr, 2010 | Pubmed ID: 20374071 P2X7 Receptor Signaling Pathway As a Therapeutic Target for Neurodegenerative Diseases Archivum Immunologiae Et Therapiae Experimentalis. Apr, 2010 | Pubmed ID: 20143170 Gamma-Irradiation Induces P2X(7) Receptor-dependent ATP Release from B16 Melanoma Cells Biochimica Et Biophysica Acta. Jan, 2010 | Pubmed ID: 19854240 Possible Roles of Microglial Cells for Neurotoxicity in Clinical Neurodegenerative Diseases and Experimental Animal Models Inflammation & Allergy Drug Targets. Sep, 2009 | Pubmed ID: 19754411 Modulation of the ATP-lnduced Release and Processing of IL-1beta in Microglial Cells Critical Reviews in Immunology. 2009 | Pubmed ID: 19673687 Characteristics of Novel Insect Defensin-based Membrane-disrupting Trypanocidal Peptides Bioscience, Biotechnology, and Biochemistry. Jul, 2009 | Pubmed ID: 19584534 The Role of the P2X7 Receptor Signaling Pathway for the Release of Autolysosomes in Microglial Cells Autophagy. Jul, 2009 | Pubmed ID: 19337025 Synthetic Nonamer Peptides Derived from Insect Defensin Mediate the Killing of African Trypanosomes in Axenic Culture Parasitology Research. Jul, 2009 | Pubmed ID: 19308456 The Activation of P2X7 Receptor Impairs Lysosomal Functions and Stimulates the Release of Autophagolysosomes in Microglial Cells Journal of Immunology (Baltimore, Md. : 1950). Feb, 2009 | Pubmed ID: 19201858 Microglial Activation is Inhibited by Corticosterone in Dopaminergic Neurodegeneration Journal of Neuroimmunology. Mar, 2009 | Pubmed ID: 19201037 Cytoplasmic Expression and Specific Binding of the VH/VL Single Domain Intrabodies in Transfected NIH3T3 Cells Experimental and Molecular Pathology. Feb, 2009 | Pubmed ID: 19094983 Establishment of an SV40 Large T Antigen-immortalized Bovine Brain Cell Line and Its Neuronal Differentiation by Dibutyryl-cyclic AMP Cell Biology International. Feb, 2009 | Pubmed ID: 19032985 Lactoferrin Induces Cell Surface Retention of Prion Protein and Inhibits Prion Accumulation Journal of Neurochemistry. Nov, 2008 | Pubmed ID: 18717818 [Establishment and Characterization of Prion-infected Microglial Cells] Seikagaku. The Journal of Japanese Biochemical Society. Jul, 2008 | Pubmed ID: 18712065 Lysophospholipids and ATP Mutually Suppress Maturation and Release of IL-1 Beta in Mouse Microglial Cells Using a Rho-dependent Pathway Journal of Immunology (Baltimore, Md. : 1950). Jun, 2008 | Pubmed ID: 18523246 Activin As an Anti-inflammatory Cytokine Produced by Microglia Journal of Neuroimmunology. Dec, 2007 | Pubmed ID: 17976743 Impaired LPS-induced Signaling in Microglia Overexpressing the Wiskott-Aldrich Syndrome Protein N-terminal Domain International Immunology. Aug, 2007 | Pubmed ID: 17698982 Prion Infection Correlates with Hypersensitivity of P2X7 Nucleotide Receptor in a Mouse Microglial Cell Line FEBS Letters. Jun, 2007 | Pubmed ID: 17544414 Lysophosphatidylcholine Potentiates Ca2+ Influx, Pore Formation and P44/42 MAP Kinase Phosphorylation Mediated by P2X7 Receptor Activation in Mouse Microglial Cells Journal of Neurochemistry. Sep, 2007 | Pubmed ID: 17437542 Roles of NF-kappaB and MAPK Signaling Pathways in Morphological and Cytoskeletal Responses of Microglia to Double-stranded RNA Neuroscience Letters. Mar, 2007 | Pubmed ID: 17284350 Microglial Cell Line Established from Prion Protein-overexpressing Mice is Susceptible to Various Murine Prion Strains Journal of Virology. Feb, 2007 | Pubmed ID: 17121794 Establishment and Characterization of SV40 Large T Antigen-immortalized Cell Lines Derived from Fetal Bovine Brain Tissues After Prolonged Cryopreservation Cell Biology International. Jan, 2007 | Pubmed ID: 17049468 Suppressive Effect of Simvastatin on Interferon-beta-induced Expression of CC Chemokine Ligand 5 in Microglia Neuroscience Letters. Oct, 2006 | Pubmed ID: 16978784 Mouse Embryonic Stem Cells Are Not Susceptible to Cytomegalovirus but Acquire Susceptibility During Differentiation Birth Defects Research. Part A, Clinical and Molecular Teratology. Feb, 2006 | Pubmed ID: 16470616 Protective Effects of Antimicrobial Peptides Derived from the Beetle Allomyrina Dichotoma Defensin on Endotoxic Shock in Mice International Immunopharmacology. Feb, 2006 | Pubmed ID: 16399628 Intrabodies Against the EVH1 Domain of Wiskott-Aldrich Syndrome Protein Inhibit T Cell Receptor Signaling in Transgenic Mice T Cells The FEBS Journal. Dec, 2005 | Pubmed ID: 16302976 Inhibitory Effects of U73122 and U73343 on Ca2+ Influx and Pore Formation Induced by the Activation of P2X7 Nucleotide Receptors in Mouse Microglial Cell Line Biochimica Et Biophysica Acta. Nov, 2005 | Pubmed ID: 16122875 Susceptibility of TNF-alpha-deficient Mice to Trypanosoma Congolense is Not Due to a Defective Antibody Response Acta Tropica. Nov-Dec, 2004 | Pubmed ID: 15533287 The Secretion of Acute Phase Proteins and Inflammatory Cytokines During Trypanosoma Congolense Infection is Not Affected by the Absence of the TNF-alpha Gene Acta Tropica. Sep, 2004 | Pubmed ID: 15301973 Evaluation of the Delta Subunit of Bovine Adaptor Protein Complex 3 As a Receptor for Bovine Leukaemia Virus The Journal of General Virology. May, 2003 | Pubmed ID: 12692298 Recombinant Tumor Necrosis Factor Alpha Does Not Inhibit the Growth of African Trypanosomes in Axenic Cultures Infection and Immunity. Apr, 2002 | Pubmed ID: 11895989 A Simple and Efficient Method to Isolate Macrophages from Mixed Primary Cultures of Adult Liver Cells Hiroshi Kitani1, Takato Takenouchi1, Mitsuru Sato1, Miyako Yoshioka2, Noriko Yamanaka2 1Transgenic Animal Research Center, National Institute of Agrobiological Sciences, Tsukuba, Japan, 2Safety Research Team, National Institute of Animal Health, Tsukuba, Japan JoVE 2757 Immunology and Infection
A Simple and Efficient Method to Isolate Macrophages from Mixed Primary Cultures of Adult Liver Cells Hiroshi Kitani1, Takato Takenouchi1, Mitsuru Sato1, Miyako Yoshioka2, Noriko Yamanaka2 1Transgenic Animal Research Center, National Institute of Agrobiological Sciences, Tsukuba, Japan, 2Safety Research Team, National Institute of Animal Health, Tsukuba, Japan JoVE 2757 Immunology and Infection