In JoVE (1)
Other Publications (1)
Articles by Holger Fehlauer in JoVE
Using a Microfluidics Device for Mechanical Stimulation and High Resolution Imaging of C. elegans Holger Fehlauer*1, Adam L. Nekimken*1,2, Anna A. Kim1,2, Beth L. Pruitt1,2,3, Miriam B. Goodman1,2, Michael Krieg4 1Department of Molecular and Cellular Physiology, Stanford University, 2Department of Mechanical Engineering, Stanford University, 3Department of Bioengineering, Stanford University, 4Group of Neurophotonics and Mechanical Systems Biology, The Institute of Photonic Sciences (ICFO) New tools for mechanobiology research are needed to understand how mechanical stress activates biochemical pathways and elicits biological responses. Here, we showcase a new method for selective mechanical stimulation of immobilized animals with a microfluidic trap allowing high-resolution imaging of cellular responses.
Other articles by Holger Fehlauer on PubMed
Pneumatic Stimulation of C. Elegans Mechanoreceptor Neurons in a Microfluidic Trap Lab on a Chip. | Pubmed ID: 28207921 New tools for applying force to animals, tissues, and cells are critically needed in order to advance the field of mechanobiology, as few existing tools enable simultaneous imaging of tissue and cell deformation as well as cellular activity in live animals. Here, we introduce a novel microfluidic device that enables high-resolution optical imaging of cellular deformations and activity while applying precise mechanical stimuli to the surface of the worm's cuticle with a pneumatic pressure reservoir. To evaluate device performance, we compared analytical and numerical simulations conducted during the design process to empirical measurements made with fabricated devices. Leveraging the well-characterized touch receptor neurons (TRNs) with an optogenetic calcium indicator as a model mechanoreceptor neuron, we established that individual neurons can be stimulated and that the device can effectively deliver steps as well as more complex stimulus patterns. This microfluidic device is therefore a valuable platform for investigating the mechanobiology of living animals and their mechanosensitive neurons.