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Articles by Hong Cui in JoVE
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Elektroporation av funktionella Bakteriella Effektorer i däggdjursceller
Ryan L. Sontag1, Cosmin Mihai2, Galya Orr2, Alexei Savchenko3, Tatiana Skarina3, Hong Cui3, John R. Cort1, Joshua N. Adkins1, Roslyn N. Brown4
1Biological Sciences Division, Pacific Northwest National Laboratory, 2Environmental Molecular Science Laboratory, Pacific Northwest National Laboratory, 3Structural Proteomics Group, Ontario Center for Structural Proteomics, University of Toronto, 4Center for Bioproducts and Bioenergy, Washington State University
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Karakterisering av glykoproteiner med immunglobulin luckan av röntgenkristallografi och biofysiska tekniker
June Ereño-Orbea*1, Taylor Sicard*1,2, Hong Cui1, Indira Akula1, Jean-Philippe Julien1,2,3
1Program in Molecular Medicine, The Hospital for Sick Children Research Institute, 2Department of Biochemistry, University of Toronto, 3Department of Immunology, University of Toronto
Vi presenterar strategier för biofysiska och strukturella karakterisering av glykoproteiner med immunglobulin luckan genom biolayer interferometri, isotermiska titrering kalorimetri och röntgenkristallografi.
Other articles by Hong Cui on PubMed
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Discovery of Ubiquitin Deamidases in the Pathogenic Arsenal of Legionella Pneumophila
Cell Reports.
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Pubmed ID: 29642013 Legionella pneumophila translocates the largest known arsenal of over 330 pathogenic factors, called "effectors," into host cells during infection, enabling L. pneumophila to establish a replicative niche inside diverse amebas and human macrophages. Here, we reveal that the L. pneumophila effectors MavC (Lpg2147) and MvcA (Lpg2148) are structural homologs of cycle inhibiting factor (Cif) effectors and that the adjacent gene, lpg2149, produces a protein that directly inhibits their activity. In contrast to canonical Cifs, both MavC and MvcA contain an insertion domain and deamidate the residue Gln40 of ubiquitin but not Gln40 of NEDD8. MavC and MvcA are functionally diverse, with only MavC interacting with the human E2-conjugating enzyme UBE2N (Ubc13). MavC deamidates the UBE2N∼Ub conjugate, disrupting Lys63 ubiquitination and dampening NF-κB signaling. Combined, our data reveal a molecular mechanism of host manipulation by pathogenic bacteria and highlight the complex regulatory mechanisms integral to L. pneumophila's pathogenic strategy.
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