Articles by Hsin-pin Ho Liang in JoVE

• Immunology and Infection

  
  An In Vitro Caseum Binding Assay that Predicts Drug Penetration in Tuberculosis Lesions Jansy P. Sarathy¹, Hsin-pin Ho Liang¹, Danielle Weiner², Jacqueline Gonzales², Laura E. Via², Véronique Dartois^{1 1}Public Health Research Institute Centre, New Jersey Medical School, Rutgers, ²Tuberculosis Research Section, Laboratory of Clinical Infectious Diseases, NIAID, NIH Here we describe a rapid equilibrium dialysis (RED) method to measure drug binding to caseum from pulmonary tuberculosis lesions and cavities. The protocol is also used with a foamy macrophage-derived matrix that is an effective surrogate to caseum.