Articles by Hyung Kyung Kim in JoVE
A Protocol to Acquire the Degenerative Tenocyte from Humans Soo-Hong Han*1, Hyung Kyung Kim*2, Jong-Ho Ahn*1, Dong Hyeon Lee3, Minjung Baek1, Geunhee Ye1, Joong-Myung Lee1, Kyunghoon Min4, Chihoon Oh1, Soonchul Lee1 1Department of Orthopaedic Surgery, CHA Bundang Medical Center, CHA University School of Medicine, 2Department of Pathology, Kyung Hee University Hospital at Gangdong, Kyung Hee University School of Medicine, 3Department of Physiology, CHA University School of Medicine, 4Department of Rehabilitation, CHA Bundang Medical Center, CHA University School of Medicine In vitro use of degenerative tenocytes is essential when investigating the efficacy of novel treatment on tendinopathy. However, most research studies use only the animal model or a healthy tenocyte. We propose the following protocol to isolate human degenerative tenocytes during surgery.
Other articles by Hyung Kyung Kim on PubMed
Prognostic Value of KRAS Codon 13 Gene Mutation for Overall Survival in Colorectal Cancer: Direct and Indirect Comparison Meta-analysis Medicine. | Pubmed ID: 28858102 The clinical significance of KRAS codon 13 mutation in patients with colorectal cancer (CRC) remains controversial. A systematic review and meta-analysis is necessary for a more precise estimation of the predictive role of KRAS codon 13 mutations in CRC patients.
Tumoral FOXP3 Expression is Associated with Favorable Clinicopathological Variables and Good Prognosis in Gastric Adenocarcinoma: the Tumor Suppressor Function of Tumoral FOXP3 is Related with the P21 Expression in Gastric Adenocarcinoma Human Pathology. | Pubmed ID: 28882702 The function and contribution of tumoral FOXP3 in gastric cancer development remain poorly understood. Thus, we studied the expression of tumoral FOXP3 and its relationship with the well-known tumor suppressor proteins P21 and P53 in gastric adenocarcinoma. The tissue microarray was constructed from 182 cases of gastric adenocarcinoma. The immunohistochemistry was performed on 4-μm tissue sections from each tissue microarray block. We found that positive tumoral FOXP3 expression was significantly correlated with a lower T category, a lower N category, a lower recurrence rate, and less lymphatic invasion. Furthermore, the survival analysis revealed that the tumoral FOXP3-positive group had significantly increased overall survival and disease-free survival rates compared with the tumoral FOXP3-negative group. Additionally, P21 expression showed a significant positive correlation with tumoral FOXP3 expression in gastric adenocarcinoma cells. Taken together, these findings demonstrate that tumoral FOXP3 expression is associated with favorable clinicopathological variables and good prognosis in gastric adenocarcinoma through increased expression of the tumor suppression protein P21.