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Articles by Inna S. Yanez-Orozco in JoVE
High Precision FRET at Single-molecule Level for Biomolecule Structure Determination Junyan Ma1, Inna S. Yanez-Orozco2, Soheila Rezaei Adariani2, Drew Dolino3, Vasanthi Jayaraman3, Hugo Sanabria2 1Department of Chemistry, Clemson University, 2Department of Physics and Astronomy, Clemson University, 3Department of Biochemistry and Molecular Biology, Center for Membrane Biology, Graduate School for Biomedical Sciences, University of Texas Health Science Center A protocol for high-precision FRET experiments at the single molecule level is presented here. Additionally, this methodology can be used to identify three conformational states in the ligand-binding domain of the N-methyl-D-aspartate (NMDA) receptor. Determining precise distances is the first step towards building structural models based on FRET experiments.