Jürgen Hubbuch Department of Biomolecular Separation Engineering Karlsruhe Institute of Technology (KIT) Biography Publications Institution JoVE Articles Jürgen Hubbuch has not added a biography. If you are Jürgen Hubbuch and would like to personalize this page please email our Author Liaison for assistance. Publications Concentration-dependent Changes in Apparent Diffusion Coefficients As Indicator for Colloidal Stability of Protein Solutions International Journal of Pharmaceutics. Jul, 2016 | Pubmed ID: 27421911 A Versatile Noninvasive Method for Adsorber Quantification in Batch and Column Chromatography Based on the Ionic Capacity Biotechnology Progress. May, 2016 | Pubmed ID: 27324662 Prediction of Salt Effects on Protein Phase Behavior by HIC Retention and Thermal Stability Journal of Pharmaceutical and Biomedical Analysis. Sep, 2016 | Pubmed ID: 27268946 Impact of Polymer Surface Characteristics on the Microrheological Measurement Quality of Protein Solutions - A Tracer Particle Screening International Journal of Pharmaceutics. May, 2016 | Pubmed ID: 27025292 Application of Spectral Deconvolution and Inverse Mechanistic Modelling As a Tool for Root Cause Investigation in Protein Chromatography Journal of Chromatography. A. Mar, 2016 | Pubmed ID: 26879457 Predictive Approach for Protein Aggregation: Correlation of Protein Surface Characteristics and Conformational Flexibility to Protein Aggregation Propensity Biotechnology and Bioengineering. Feb, 2016 | Pubmed ID: 26853436 High-throughput Characterization of Virus-like Particles by Interlaced Size-exclusion Chromatography Vaccine. Mar, 2016 | Pubmed ID: 26845741 High-throughput Cell Quantification Assays for Use in Cell Purification Development - Enabling Technologies for Cell Production Biotechnology Journal. May, 2016 | Pubmed ID: 26814049 Modeling and Simulation of Anion-exchange Membrane Chromatography for Purification of Sf9 Insect Cell-derived Virus-like Particles Journal of Chromatography. A. Jan, 2016 | Pubmed ID: 26718185 High-throughput Process Development of an Alternative Platform for the Production of Virus-like Particles in Escherichia Coli Journal of Biotechnology. Feb, 2016 | Pubmed ID: 26707548 Squeeze Flow Rheometry As a Novel Tool for the Characterization of Highly Concentrated Protein Solutions Biotechnology and Bioengineering. Mar, 2016 | Pubmed ID: 26375304 The Influence of Mixed Salts on the Capacity of HIC Adsorbers: A Predictive Correlation to the Surface Tension and the Aggregation Temperature Biotechnology Progress. Mar, 2016 | Pubmed ID: 26358156 Effect of Lysozyme Solid-phase PEGylation on Reaction Kinetics and Isoform Distribution Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences. Oct, 2015 | Pubmed ID: 26359552 Custom-tailored Adsorbers: A Molecular Dynamics Study on Optimal Design of Ion Exchange Chromatography Material Journal of Chromatography. A. Sep, 2015 | Pubmed ID: 26319376 Manipulation of Lysozyme Phase Behavior by Additives As Function of Conformational Stability International Journal of Pharmaceutics. Oct, 2015 | Pubmed ID: 26302861 Non-invasive High Throughput Approach for Protein Hydrophobicity Determination Based on Surface Tension Biotechnology and Bioengineering. Dec, 2015 | Pubmed ID: 26074500 High-throughput Micro-scale Cultivations and Chromatography Modeling: Powerful Tools for Integrated Process Development Biotechnology and Bioengineering. Oct, 2015 | Pubmed ID: 25988478 High-throughput Process Development of Purification Alternatives for the Protein Avidin Biotechnology Progress. Jul-Aug, 2015 | Pubmed ID: 25958993 Influence of Binding PH and Protein Solubility on the Dynamic Binding Capacity in Hydrophobic Interaction Chromatography Journal of Chromatography. A. May, 2015 | Pubmed ID: 25911386 Light Extinction and Scattering by Agarose Based Resin Beads and Applications in High-throughput Screening Journal of Chromatography. A. Jun, 2015 | Pubmed ID: 25900741 Next Generation Vaccines and Vectors: Designing Downstream Processes for Recombinant Protein-based Virus-like Particles Biotechnology Journal. May, 2015 | Pubmed ID: 25880158 Robust High-throughput Batch Screening Method in 384-well Format with Optical In-line Resin Quantification Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences. Apr, 2015 | Pubmed ID: 25765136 Integrated Development of Up- and Downstream Processes Supported by the Cherry-Tag™ for Real-time Tracking of Stability and Solubility of Proteins Journal of Biotechnology. Apr, 2015 | Pubmed ID: 25747171 Advances in Inline Quantification of Co-eluting Proteins in Chromatography: Process-data-based Model Calibration and Application Towards Real-life Separation Issues Biotechnology and Bioengineering. Jul, 2015 | Pubmed ID: 25683378 Downstream Processing of Virus-like Particles: Single-stage and Multi-stage Aqueous Two-phase Extraction Journal of Chromatography. A. Feb, 2015 | Pubmed ID: 25637013 A Comprehensive Molecular Dynamics Approach to Protein Retention Modeling in Ion Exchange Chromatography Journal of Chromatography. A. Feb, 2015 | Pubmed ID: 25618359 Determination of Protein Phase Diagrams by Microbatch Experiments: Exploring the Influence of Precipitants and PH International Journal of Pharmaceutics. Feb, 2015 | Pubmed ID: 25541147 Computational Study of Elements of Stability of a Four-helix Bundle Protein Biosurfactant Journal of Computer-aided Molecular Design. Jan, 2015 | Pubmed ID: 25323391 Characterization of Aqueous Two Phase Systems by Combining Lab-on-a-chip Technology with Robotic Liquid Handling Stations Journal of Chromatography. A. Nov, 2014 | Pubmed ID: 25280873 Soluble Full-length Expression and Characterization of SnRNP Protein U1-68/70K Protein Expression and Purification. Dec, 2014 | Pubmed ID: 25201698 Moving Through Three-dimensional Phase Diagrams of Monoclonal Antibodies Biotechnology Progress. Sep-Oct, 2014 | Pubmed ID: 25044865 A Tool for Selective Inline Quantification of Co-eluting Proteins in Chromatography Using Spectral Analysis and Partial Least Squares Regression Biotechnology and Bioengineering. Jul, 2014 | Pubmed ID: 24522836 Molecular Dynamics Simulations Approach for the Characterization of Peptides with Respect to Hydrophobicity The Journal of Physical Chemistry. B. Feb, 2014 | Pubmed ID: 24506060 Optimization of Random PEGylation Reactions by Means of High Throughput Screening Biotechnology and Bioengineering. Jan, 2014 | Pubmed ID: 23939788 Alternative Separation Steps for Monoclonal Antibody Purification: Combination of Centrifugal Partitioning Chromatography and Precipitation Journal of Chromatography. A. Dec, 2013 | Pubmed ID: 24182866 A High-throughput 2D-analytical Technique to Obtain Single Protein Parameters from Complex Cell Lysates for in Silico Process Development of Ion Exchange Chromatography Journal of Chromatography. A. Nov, 2013 | Pubmed ID: 24139506 Analytical Characterization of Complex, Biotechnological Feedstocks by PH Gradient Ion Exchange Chromatography for Purification Process Development Journal of Chromatography. A. Oct, 2013 | Pubmed ID: 24016717 Microfluidics on Liquid Handling Stations (μF-on-LHS): an Industry Compatible Chip Interface Between Microfluidics and Automated Liquid Handling Stations Lab on a Chip. Jun, 2013 | Pubmed ID: 23639992 Systematic Generation of Buffer Systems for PH Gradient Ion Exchange Chromatography and Their Application Journal of Chromatography. A. Apr, 2013 | Pubmed ID: 23489486 Accurate Retention Time Determination of Co-eluting Proteins in Analytical Chromatography by Means of Spectral Data Biotechnology and Bioengineering. Mar, 2013 | Pubmed ID: 23042430 Selective High Throughput Protein Quantification Based on UV Absorption Spectra Biotechnology and Bioengineering. Feb, 2013 | Pubmed ID: 22903696 Isoform Separation and Binding Site Determination of Mono-PEGylated Lysozyme with PH Gradient Chromatography Journal of Chromatography. A. Dec, 2012 | Pubmed ID: 23146338 Rapid Quantification of Protein-polyethylene Glycol Conjugates by Multivariate Evaluation of Chromatographic Data Journal of Chromatography. A. Sep, 2012 | Pubmed ID: 22920306 High-throughput Screening-based Selection and Scale-up of Aqueous Two-phase Systems for PDNA Purification Journal of Separation Science. Nov, 2012 | Pubmed ID: 22888101 Molecular Dynamics Simulations on Aqueous Two-phase Systems - Single PEG-molecules in Solution BMC Biophysics. 2012 | Pubmed ID: 22873343 Development and Characterization of an Automated High Throughput Screening Method for Optimization of Protein Refolding Processes Journal of Separation Science. Nov, 2012 | Pubmed ID: 22821717 High Throughput Screening Based Selection of Phases for Aqueous Two-phase System-centrifugal Partitioning Chromatography of Monoclonal Antibodies Journal of Chromatography. A. Aug, 2012 | Pubmed ID: 22789754 Examination of a Genetic Algorithm for the Application in High-throughput Downstream Process Development Biotechnology Journal. Oct, 2012 | Pubmed ID: 22700464 High-throughput Methods for Miniaturization and Automation of Monoclonal Antibody Purification Processes Biotechnology Progress. May-Jun, 2012 | Pubmed ID: 22467605 A Sub-two Minutes Method for Monoclonal Antibody-aggregate Quantification Using Parallel Interlaced Size Exclusion High Performance Liquid Chromatography Journal of Chromatography. A. Dec, 2011 | Pubmed ID: 22078232 A Label-free Methodology for Selective Protein Quantification by Means of Absorption Measurements Biotechnology and Bioengineering. Nov, 2011 | Pubmed ID: 21755495 Application of an Aqueous Two-phase Systems High-throughput Screening Method to Evaluate MAb HCP Separation Biotechnology and Bioengineering. Jan, 2011 | Pubmed ID: 20717969 Rational and Systematic Protein Purification Process Development: the Next Generation Trends in Biotechnology. Dec, 2009 | Pubmed ID: 19815300 Effects of Ionic Strength and Mobile Phase PH on the Binding Orientation of Lysozyme on Different Ion-exchange Adsorbents Journal of Chromatography. A. Jun, 2008 | Pubmed ID: 18234205 Confocal Laser Scanning Microscopy As an Analytical Tool in Chromatographic Research Bioprocess and Biosystems Engineering. Apr, 2008 | Pubmed ID: 18196281 Comparison of Chromatographic Ion-exchange Resins VI. Weak Anion-exchange Resins Journal of Chromatography. A. Sep, 2007 | Pubmed ID: 17658538 A Novel Two-zone Protein Uptake Model for Affinity Chromatography and Its Application to the Description of Elution Band Profiles of Proteins Fused to a Family 9 Cellulose Binding Module Affinity Tag Journal of Chromatography. A. Aug, 2007 | Pubmed ID: 17602694 A Novel Approach to Characterize the Binding Orientation of Lysozyme on Ion-exchange Resins Journal of Chromatography. A. May, 2007 | Pubmed ID: 17439816 Investigation of Pore Diffusion Hindrance of Monoclonal Antibody in Hydrophobic Interaction Chromatography Using Confocal Laser Scanning Microscopy Journal of Chromatography. A. May, 2007 | Pubmed ID: 17418853 High-throughput Screening of Packed-bed Chromatography Coupled with SELDI-TOF MS Analysis: Monoclonal Antibodies Versus Host Cell Protein Biotechnology and Bioengineering. Oct, 2007 | Pubmed ID: 17335062 Changes in Retention Behavior of Fluorescently Labeled Proteins During Ion-exchange Chromatography Caused by Different Protein Surface Labeling Positions Biotechnology and Bioengineering. Sep, 2007 | Pubmed ID: 17318908 Separation of Genomic DNA, RNA, and Open Circular Plasmid DNA from Supercoiled Plasmid DNA by Combining Denaturation, Selective Renaturation and Aqueous Two-phase Extraction Biotechnology and Bioengineering. Jan, 2007 | Pubmed ID: 16937402 Short-cut Method for the Correction of Light Attenuation Influences in the Experimental Data Obtained from Confocal Laser Scanning Microscopy Journal of Chromatography. A. Dec, 2006 | Pubmed ID: 17078960 Developing a Chromatographic Column Model for Bovine Serum Albumin on Strong Anion-exchanger Source30Q Using Data from Confocal Laser Scanning Microscopy Journal of Chromatography. A. Dec, 2006 | Pubmed ID: 17055517 Competitive Adsorption of Labeled and Native Protein in Confocal Laser Scanning Microscopy Biotechnology and Bioengineering. Sep, 2006 | Pubmed ID: 16752371 Generation of Equally Sized Particle Plaques Using Solid-liquid Suspensions Biotechnology Progress. May-Jun, 2006 | Pubmed ID: 16739980 Exploitation of the Coil-globule Plasmid DNA Transition Induced by Small Changes in Temperature, PH Salt, and Poly(ethylene Glycol) Compositions for Directed Partitioning in Aqueous Two-phase Systems Langmuir : the ACS Journal of Surfaces and Colloids. Apr, 2006 | Pubmed ID: 16618176 The Influence of Homogenisation Conditions on Biomass-adsorbent Interactions During Ion-exchange Expanded Bed Adsorption Biotechnology and Bioengineering. Jun, 2006 | Pubmed ID: 16518839 Direct Quantification of Intraparticle Protein Diffusion in Chromatographic Media The Journal of Physical Chemistry. B. Jan, 2006 | Pubmed ID: 16471694 Protein-labeling Effects in Confocal Laser Scanning Microscopy The Journal of Physical Chemistry. B. Jul, 2005 | Pubmed ID: 16852729 Integrated Bioprocesses Current Opinion in Microbiology. Jun, 2005 | Pubmed ID: 15939352 Biochemical Engineering Aspects of Expanded Bed Adsorption Advances in Biochemical Engineering/biotechnology. 2005 | Pubmed ID: 15791934 Scalable Recovery of Plasmid DNA Based on Aqueous Two-phase Separation Biotechnology and Applied Biochemistry. Aug, 2005 | Pubmed ID: 15612880 The Influence of Biomass on the Hydrodynamic Behavior and Stability of Expanded Beds Biotechnology and Bioengineering. Aug, 2004 | Pubmed ID: 15281108 Superparamagnetic Adsorbents for High-gradient Magnetic Fishing of Lectins out of Legume Extracts Biotechnology and Bioengineering. Aug, 2004 | Pubmed ID: 15281106 Mechanism and Kinetics of Protein Transport in Chromatographic Media Studied by Confocal Laser Scanning Microscopy. Part II. Impact on Chromatographic Separations Journal of Chromatography. A. Dec, 2003 | Pubmed ID: 14735979 Mechanism and Kinetics of Protein Transport in Chromatographic Media Studied by Confocal Laser Scanning Microscopy. Part I. The Interplay of Sorbent Structure and Fluid Phase Conditions Journal of Chromatography. A. Dec, 2003 | Pubmed ID: 14735978 Biomass/adsorbent Electrostatic Interactions in Expanded Bed Adsorption: a Zeta Potential Study Biotechnology and Bioengineering. Jul, 2003 | Pubmed ID: 12768620 Dynamics of Protein Uptake Within the Adsorbent Particle During Packed Bed Chromatography Biotechnology and Bioengineering. Nov, 2002 | Pubmed ID: 12325144 High-gradient Magnetic Affinity Separation of Trypsin from Porcine Pancreatin Biotechnology and Bioengineering. Aug, 2002 | Pubmed ID: 12115419 A New Fluid Distribution System for Scale-flexible Expanded Bed Adsorption Biotechnology and Bioengineering. Apr, 2002 | Pubmed ID: 11857279 無傷の植物をブランチングすることによって、または抽出物の熱処理によりタバコ宿主細胞タンパク質の除去方法の比較 Johannes F. Buyel1,2, Jürgen Hubbuch3, Rainer Fischer1,2 1Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e. V., 2Institute for Molecular Biotechnology, RWTH Aachen University, 3Department of Biomolecular Separation Engineering, Karlsruhe Institute of Technology (KIT) JoVE 54343 Biology
無傷の植物をブランチングすることによって、または抽出物の熱処理によりタバコ宿主細胞タンパク質の除去方法の比較 Johannes F. Buyel1,2, Jürgen Hubbuch3, Rainer Fischer1,2 1Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e. V., 2Institute for Molecular Biotechnology, RWTH Aachen University, 3Department of Biomolecular Separation Engineering, Karlsruhe Institute of Technology (KIT) JoVE 54343 Biology
