James B. Hurley Department of Biochemistry University of Washington Biography Publications Institution JoVE Articles James B. Hurley has not added a biography. If you are James B. Hurley and would like to personalize this page please email our Author Liaison for assistance. Publications Aerobic Glycolysis in Photoreceptors Supports Energy Demand in the Absence of Mitochondrial Coupling Advances in Experimental Medicine and Biology. 2023 | Pubmed ID: 37440069 Proline Provides a Nitrogen Source in the Retinal Pigment Epithelium to Synthesize and Export Amino Acids for the Neural Retina BioRxiv : the Preprint Server for Biology. Apr, 2023 | Pubmed ID: 37131780 The Multi-tissue Landscape of Somatic MtDNA Mutations Indicates Tissue-specific Accumulation and Removal in Aging ELife. Feb, 2023 | Pubmed ID: 36799304 Transducin-Deficient Rod Photoreceptors Evaluated With Optical Coherence Tomography and Oxygen Consumption Rate Energy Biomarkers Investigative Ophthalmology & Visual Science. Dec, 2022 | Pubmed ID: 36576748 Succinate Metabolism in the Retinal Pigment Epithelium Uncouples Respiration from ATP Synthesis Cell Reports. Jun, 2022 | Pubmed ID: 35675773 Monocarboxylate Transporter 1 (MCT1) Mediates Succinate Export in the Retina Investigative Ophthalmology & Visual Science. Apr, 2022 | Pubmed ID: 35363247 Extracellular Matrix Dysfunction in Sorsby Patient-derived Retinal Pigment Epithelium Experimental Eye Research. Feb, 2022 | Pubmed ID: 34929159 An Analysis of Metabolic Changes in the Retina and Retinal Pigment Epithelium of Aging Mice Investigative Ophthalmology & Visual Science. Nov, 2021 | Pubmed ID: 34797906 Fluidics System for Resolving Concentration-dependent Effects of Dissolved Gases on Tissue Metabolism ELife. Nov, 2021 | Pubmed ID: 34734803 Retina Metabolism and Metabolism in the Pigmented Epithelium: A Busy Intersection Annual Review of Vision Science. Sep, 2021 | Pubmed ID: 34102066 Mitochondria: The Retina's Achilles' Heel in AMD Advances in Experimental Medicine and Biology. 2021 | Pubmed ID: 33848005 Effect of Selectively Knocking Down Key Metabolic Genes in Müller Glia on Photoreceptor Health Glia. Aug, 2021 | Pubmed ID: 33835598 Absence of Retbindin Blocks Glycolytic Flux, Disrupts Metabolic Homeostasis, and Leads to Photoreceptor Degeneration Proceedings of the National Academy of Sciences of the United States of America. Feb, 2021 | Pubmed ID: 33526685 Daily Mitochondrial Dynamics in Cone Photoreceptors Proceedings of the National Academy of Sciences of the United States of America. Nov, 2020 | Pubmed ID: 33144507 Mitochondrial Calcium Uniporter (MCU) Deficiency Reveals an Alternate Path for Ca Uptake in Photoreceptor Mitochondria Scientific Reports. 09, 2020 | Pubmed ID: 32994451 Succinate Can Shuttle Reducing Power from the Hypoxic Retina to the O-Rich Pigment Epithelium Cell Reports. May, 2020 | Pubmed ID: 32375026 Non-photopic and Photopic Visual Cycles Differentially Regulate Immediate, Early, and Late Phases of Cone Photoreceptor-mediated Vision The Journal of Biological Chemistry. May, 2020 | Pubmed ID: 32238432 Retinal Disease: How to Use Proteomics to Speed Up Diagnosis and Metabolomics to Slow Down Degeneration EBioMedicine. Mar, 2020 | Pubmed ID: 32113163 Increasing Ca in Photoreceptor Mitochondria Alters Metabolites, Accelerates Photoresponse Recovery, and Reveals Adaptations to Mitochondrial Stress Cell Death and Differentiation. 03, 2020 | Pubmed ID: 31371786 Loss of MPC1 Reprograms Retinal Metabolism to Impair Visual Function Proceedings of the National Academy of Sciences of the United States of America. Feb, 2019 | Pubmed ID: 30808746 Impact of Euthanasia, Dissection and Postmortem Delay on Metabolic Profile in Mouse Retina and RPE/choroid Experimental Eye Research. Sep, 2018 | Pubmed ID: 29864440 How Excessive CGMP Impacts Metabolic Proteins in Retinas at the Onset of Degeneration Advances in Experimental Medicine and Biology. 2018 | Pubmed ID: 29721955 Pyruvate Kinase M2 Regulates Photoreceptor Structure, Function, and Viability Cell Death & Disease. Feb, 2018 | Pubmed ID: 29445082 Biochemical Adaptations of the Retina and Retinal Pigment Epithelium Support a Metabolic Ecosystem in the Vertebrate Eye ELife. Sep, 2017 | Pubmed ID: 28901286 Warburg's Vision ELife. Jul, 2017 | Pubmed ID: 28681722 Human Retinal Pigment Epithelial Cells Prefer Proline As a Nutrient and Transport Metabolic Intermediates to the Retinal Side The Journal of Biological Chemistry. 08, 2017 | Pubmed ID: 28615447 Mitochondria Maintain Distinct Ca Pools in Cone Photoreceptors The Journal of Neuroscience : the Official Journal of the Society for Neuroscience. Feb, 2017 | Pubmed ID: 28115482 Reductive Carboxylation is a Major Metabolic Pathway in the Retinal Pigment Epithelium Proceedings of the National Academy of Sciences of the United States of America. 12, 2016 | Pubmed ID: 27911769 Reprogramming Metabolism by Targeting Sirtuin 6 Attenuates Retinal Degeneration The Journal of Clinical Investigation. Dec, 2016 | Pubmed ID: 27841758 Deficient Glucose and Glutamine Metabolism in Knockout Mice Contributes to Altered Visual Function Molecular Vision. 2016 | Pubmed ID: 27746674 Phototransduction Influences Metabolic Flux and Nucleotide Metabolism in Mouse Retina The Journal of Biological Chemistry. Feb, 2016 | Pubmed ID: 26677218 Probing Metabolism in the Intact Retina Using Stable Isotope Tracers Methods in Enzymology. 2015 | Pubmed ID: 26358904 It's Never Too Late to Save a Photoreceptor The Journal of Clinical Investigation. Sep, 2015 | Pubmed ID: 26301805 Glucose, Lactate, and Shuttling of Metabolites in Vertebrate Retinas Journal of Neuroscience Research. Jul, 2015 | Pubmed ID: 25801286 Deregulated Myc Requires MondoA/Mlx for Metabolic Reprogramming and Tumorigenesis Cancer Cell. Feb, 2015 | Pubmed ID: 25640402 Pyruvate Kinase and Aspartate-glutamate Carrier Distributions Reveal Key Metabolic Links Between Neurons and Glia in Retina Proceedings of the National Academy of Sciences of the United States of America. Oct, 2014 | Pubmed ID: 25313047 The Retinal Pigment Epithelium Utilizes Fatty Acids for Ketogenesis The Journal of Biological Chemistry. Jul, 2014 | Pubmed ID: 24898254 Inhibition of Mitochondrial Pyruvate Transport by Zaprinast Causes Massive Accumulation of Aspartate at the Expense of Glutamate in the Retina The Journal of Biological Chemistry. Dec, 2013 | Pubmed ID: 24187136 Cytosolic Reducing Power Preserves Glutamate in Retina Proceedings of the National Academy of Sciences of the United States of America. Nov, 2013 | Pubmed ID: 24127593 Linee Guida Per L'uso E L'interpretazione Delle Analisi Per Il Monitoraggio Autophagy Autophagy. Apr, 2012 | Pubmed ID: 22966490 Na(V)1.1 Channels Are Critical for Intercellular Communication in the Suprachiasmatic Nucleus and for Normal Circadian Rhythms Proceedings of the National Academy of Sciences of the United States of America. Feb, 2012 | Pubmed ID: 22223655 Roles of Glucose in Photoreceptor Survival The Journal of Biological Chemistry. Oct, 2011 | Pubmed ID: 21840997 CTCF Regola L'espressione Ataxin-7 Attraverso La Promozione Di Un RNA Non Codificante Convergently Trascritto, Antisenso Neuron. Jun, 2011 | Pubmed ID: 21689595 PRE-1, a Cis Element Sufficient to Enhance Cone- and Rod- Specific Expression in Differentiating Zebrafish Photoreceptors BMC Developmental Biology. Jan, 2011 | Pubmed ID: 21261954 Flusso Di Energia Nella Retina Esterna Nel Buio E Nella Luce Proceedings of the National Academy of Sciences of the United States of America. May, 2010 | Pubmed ID: 20445106 Trattamento Ottima Dei Fotorecettori Segnali è Necessaria Per Massimizzare La Sensibilità Comportamentale The Journal of Physiology. Jun, 2010 | Pubmed ID: 20403975 Relationships Among Visual Cycle Retinoids, Rhodopsin Phosphorylation, and Phototransduction in Mouse Eyes During Light and Dark Adaptation Biochemistry. Mar, 2010 | Pubmed ID: 20155952 Arrestin Competition Influences the Kinetics and Variability of the Single-photon Responses of Mammalian Rod Photoreceptors The Journal of Neuroscience : the Official Journal of the Society for Neuroscience. Sep, 2009 | Pubmed ID: 19776273 AIPL1, a Protein Associated with Childhood Blindness, Interacts with Alpha-subunit of Rod Phosphodiesterase (PDE6) and is Essential for Its Proper Assembly The Journal of Biological Chemistry. Nov, 2009 | Pubmed ID: 19758987 Loss of the Synaptic Vesicle Protein SV2B Results in Reduced Neurotransmission and Altered Synaptic Vesicle Protein Expression in the Retina PloS One. 2009 | Pubmed ID: 19381277 Mechanism of Light-induced Translocation of Arrestin and Transducin in Photoreceptors: Interaction-restricted Diffusion IUBMB Life. Jan, 2008 | Pubmed ID: 18379987 Regulation of Arrestin Binding by Rhodopsin Phosphorylation Level The Journal of Biological Chemistry. Nov, 2007 | Pubmed ID: 17848565 Subunit Dissociation and Diffusion Determine the Subcellular Localization of Rod and Cone Transducins The Journal of Neuroscience : the Official Journal of the Society for Neuroscience. May, 2007 | Pubmed ID: 17507570 Biochemical Function of the LCA Linked Protein, Aryl Hydrocarbon Receptor Interacting Protein Like-1 (AIPL1). Role of AIPL1 in Retina Advances in Experimental Medicine and Biology. 2006 | Pubmed ID: 17249560 Scotopic and Photopic Visual Thresholds and Spatial and Temporal Discrimination Evaluated by Behavior of Mice in a Water Maze Photochemistry and Photobiology. Nov-Dec, 2006 | Pubmed ID: 16683905 Light-dependent Redistribution of Arrestin in Vertebrate Rods is an Energy-independent Process Governed by Protein-protein Interactions Neuron. May, 2005 | Pubmed ID: 15944125 Recoverina Migliora La Visione Mediata Da Rod Potenziando La Trasmissione Del Segnale Nella Retina Del Mouse Neuron. May, 2005 | Pubmed ID: 15882641 The Zebrafish Nrc Mutant Reveals a Role for the Polyphosphoinositide Phosphatase Synaptojanin 1 in Cone Photoreceptor Ribbon Anchoring The Journal of Neuroscience : the Official Journal of the Society for Neuroscience. Oct, 2004 | Pubmed ID: 15470129 Leber Congenital Amaurosis Linked to AIPL1: a Mouse Model Reveals Destabilization of CGMP Phosphodiesterase Proceedings of the National Academy of Sciences of the United States of America. Sep, 2004 | Pubmed ID: 15365178 Zebrafish Rx3 and Mab21l2 Are Required During Eye Morphogenesis Developmental Biology. Jun, 2004 | Pubmed ID: 15183718 Functional Analyses of Mutant Recessive GUCY2D Alleles Identified in Leber Congenital Amaurosis Patients: Protein Domain Comparisons and Dominant Negative Effects Molecular Vision. Apr, 2004 | Pubmed ID: 15123990 A Zebrafish Model for Pyruvate Dehydrogenase Deficiency: Rescue of Neurological Dysfunction and Embryonic Lethality Using a Ketogenic Diet Proceedings of the National Academy of Sciences of the United States of America. Mar, 2004 | Pubmed ID: 15070761 Visual Pigment Phosphorylation but Not Transducin Translocation Can Contribute to Light Adaptation in Zebrafish Cones Neuron. Mar, 2004 | Pubmed ID: 15046724 AIPL1, a Protein Implicated in Leber's Congenital Amaurosis, Interacts with and Aids in Processing of Farnesylated Proteins Proceedings of the National Academy of Sciences of the United States of America. Oct, 2003 | Pubmed ID: 14555765 Acceleration of Key Reactions As a Strategy to Elucidate the Rate-limiting Chemistry Underlying Phototransduction Inactivation Investigative Ophthalmology & Visual Science. Mar, 2003 | Pubmed ID: 12601023 Luce Stimola Un Aumento Transducina-indipendente Di Citoplasmico Ca2 + E La Soppressione Di Corrente in Coni Dal Nof Mutante Di Zebrafish The Journal of Neuroscience : the Official Journal of the Society for Neuroscience. Jan, 2003 | Pubmed ID: 12533607 Analysis of the Visual Cycle in Cellular Retinol-binding Protein Type I (CRBPI) Knockout Mice Investigative Ophthalmology & Visual Science. Jun, 2002 | Pubmed ID: 12036972 Mass Spectrometric Analysis of the Kinetics of in Vivo Rhodopsin Phosphorylation Protein Science : a Publication of the Protein Society. Apr, 2002 | Pubmed ID: 11910029 Shedding Light on Adaptation The Journal of General Physiology. Feb, 2002 | Pubmed ID: 11815663 Normal Light Response, Photoreceptor Integrity, and Rhodopsin Dephosphorylation in Mice Lacking Both Protein Phosphatases with EF Hands (PPEF-1 and PPEF-2) Molecular and Cellular Biology. Dec, 2001 | Pubmed ID: 11713293 Folding and Subunit Assembly of Photoreceptor Peripherin/rds is Mediated by Determinants Within the Extracellular/intradiskal EC2 Domain: Implications for Heterogeneous Molecular Pathologies The Journal of Biological Chemistry. Nov, 2001 | Pubmed ID: 11553636 Biochemical Analysis of a Dimerization Domain Mutation in RetGC-1 Associated with Dominant Cone-rod Dystrophy Proceedings of the National Academy of Sciences of the United States of America. Aug, 1999 | Pubmed ID: 10430891 Abnormal Photoresponses and Light-induced Apoptosis in Rods Lacking Rhodopsin Kinase Proceedings of the National Academy of Sciences of the United States of America. Mar, 1999 | Pubmed ID: 10097103 Two Amino Acid Substitutions Convert a Guanylyl Cyclase, RetGC-1, into an Adenylyl Cyclase Proceedings of the National Academy of Sciences of the United States of America. May, 1998 | Pubmed ID: 9600905 A New Form of Inherited Red-blindness Identified in Zebrafish The Journal of Neuroscience : the Official Journal of the Society for Neuroscience. Jun, 1997 | Pubmed ID: 9151740 The Core Domain of a New Retina Specific RGS Protein Stimulates the GTPase Activity of Transducin in Vitro Proceedings of the National Academy of Sciences of the United States of America. Apr, 1997 | Pubmed ID: 9096326 Author Spotlight: Advancing Eye Physiology Research via a Multi-Channel Flow Culture for Optimal Tissue Maintenance and Real-Time Assessment Matthew K. Grumbine1, Varun Kamat2, Khang Bao1, Trevor Crupi1, Kedar Mokate2, Rayne Lim3, Jennifer R. Chao3, Brian M. Robbings4, Daniel T. Hass4, James B. Hurley4, Ian R. Sweet1,2 1EnTox Sciences, Inc, 2UW Medicine Diabetes Institute, University of Washington, 3Department of Ophthalmology, University of Washington, 4Department of Biochemistry, University of Washington JoVE 65399 Bioengineering Preparazione fette fresche della retina dallo Zebrafish adulto per Ex Vivo Imaging esperimenti Michelle M. Giarmarco1, Whitney M. Cleghorn1, James B. Hurley1,2, Susan E. Brockerhoff1,2 1Department of Biochemistry, University of Washington, 2Department of Ophthalmology, University of Washington JoVE 56977 Neuroscience
Author Spotlight: Advancing Eye Physiology Research via a Multi-Channel Flow Culture for Optimal Tissue Maintenance and Real-Time Assessment Matthew K. Grumbine1, Varun Kamat2, Khang Bao1, Trevor Crupi1, Kedar Mokate2, Rayne Lim3, Jennifer R. Chao3, Brian M. Robbings4, Daniel T. Hass4, James B. Hurley4, Ian R. Sweet1,2 1EnTox Sciences, Inc, 2UW Medicine Diabetes Institute, University of Washington, 3Department of Ophthalmology, University of Washington, 4Department of Biochemistry, University of Washington JoVE 65399 Bioengineering
Preparazione fette fresche della retina dallo Zebrafish adulto per Ex Vivo Imaging esperimenti Michelle M. Giarmarco1, Whitney M. Cleghorn1, James B. Hurley1,2, Susan E. Brockerhoff1,2 1Department of Biochemistry, University of Washington, 2Department of Ophthalmology, University of Washington JoVE 56977 Neuroscience
