James R. Sellers Laboratory of Molecular Physiology, Cell and Developmental Biology Center, National Heart, Lung and Blood Institute National Institutes of Health Biography Publications Institution JoVE Articles James R. Sellers has not added a biography. If you are James R. Sellers and would like to personalize this page please email our Author Liaison for assistance. Publications A Binding Protein Regulates Myosin-7a Dimerization and Actin Bundle Assembly Nature Communications. 01, 2021 | Pubmed ID: 33495456 The ATPase Mechanism of Myosin 15, the Molecular Motor Mutated in DFNB3 Human Deafness The Journal of Biological Chemistry. Jan, 2021 | Pubmed ID: 33428946 The ATPase Mechanism of Myosin 15, the Molecular Motor Mutated in DFNB3 Human Deafness The Journal of Biological Chemistry. Dec, 2020 | Pubmed ID: 33372036 Tyrosine Phosphorylation of the Myosin Regulatory Light Chain Controls Non-muscle Myosin II Assembly and Function in Migrating Cells Current Biology : CB. Jul, 2020 | Pubmed ID: 32502416 How Myosin 5 Walks Deduced from Single-Molecule Biophysical Approaches Advances in Experimental Medicine and Biology. 2020 | Pubmed ID: 32451859 Megakaryocyte Migration Defects Due to Nonmuscle Myosin IIA Mutations Underlie Thrombocytopenia in MYH9-related Disease Blood. 05, 2020 | Pubmed ID: 32315395 Competition Between Kinesin-1 and Myosin-V Defines Posterior Determination ELife. Feb, 2020 | Pubmed ID: 32057294 Perfringolysin O-Induced Plasma Membrane Pores Trigger Actomyosin Remodeling and Endoplasmic Reticulum Redistribution Toxins. Jul, 2019 | Pubmed ID: 31319618 Remarkable Rigidity of the Single α-Helical Domain of Myosin-VI As Revealed by NMR Spectroscopy Journal of the American Chemical Society. Jun, 2019 | Pubmed ID: 31117653 Nonmuscle Myosin-2 Isoforms Current Biology : CB. Apr, 2019 | Pubmed ID: 31014482 Multiple S100 Protein Isoforms and C-terminal Phosphorylation Contribute to the Paralog-selective Regulation of Nonmuscle Myosin 2 Filaments The Journal of Biological Chemistry. 09, 2018 | Pubmed ID: 30087119 Dissecting Myosin-5B Mechanosensitivity and Calcium Regulation at the Single Molecule Level Nature Communications. 07, 2018 | Pubmed ID: 30030431 Bipolar Filaments of Human Nonmuscle Myosin 2-A and 2-B Have Distinct Motile and Mechanical Properties ELife. 02, 2018 | Pubmed ID: 29419377 Mechanistic Insights into the Active Site and Allosteric Communication Pathways in Human Nonmuscle Myosin-2C ELife. 12, 2017 | Pubmed ID: 29256864 Kinetic Signatures of Myosin-5B, the Motor Involved in Microvillus Inclusion Disease The Journal of Biological Chemistry. 11, 2017 | Pubmed ID: 28882893 Effect of ATP and Regulatory Light-chain Phosphorylation on the Polymerization of Mammalian Nonmuscle Myosin II Proceedings of the National Academy of Sciences of the United States of America. 08, 2017 | Pubmed ID: 28739905 Re-evaluating the Roles of Myosin 18Aα and F-actin in Determining Golgi Morphology Cytoskeleton (Hoboken, N.J.). May, 2017 | Pubmed ID: 28329908 Local Pulsatile Contractions Are an Intrinsic Property of the Myosin 2A Motor in the Cortical Cytoskeleton of Adherent Cells Molecular Biology of the Cell. 01, 2017 | Pubmed ID: 27881665 Non-muscle Myosin II Motor Activity Determines the Rate of Tissue Folding ELife. 12, 2016 | Pubmed ID: 28035903 Mammalian Nonmuscle Myosin II Binds to Anionic Phospholipids with Concomitant Dissociation of the Regulatory Light Chain The Journal of Biological Chemistry. Nov, 2016 | Pubmed ID: 27697842 Mechanisms and Functional Diversity of Macromolecular Remodeling by ATP-Dependent Motors Journal of Molecular Biology. 05, 2016 | Pubmed ID: 27113061 Kinetic Adaptations of Myosins for Their Diverse Cellular Functions Traffic (Copenhagen, Denmark). 08, 2016 | Pubmed ID: 26929436 The Arf GTPase-activating Protein, ASAP1, Binds Nonmuscle Myosin 2A to Control Remodeling of the Actomyosin Network The Journal of Biological Chemistry. Apr, 2016 | Pubmed ID: 26893376 Various Themes of Myosin Regulation Journal of Molecular Biology. 05, 2016 | Pubmed ID: 26827725 Myosin 18A Coassembles with Nonmuscle Myosin 2 to Form Mixed Bipolar Filaments Current Biology : CB. Mar, 2015 | Pubmed ID: 25754640 Structural Dynamics of Myosin 5 During Processive Motion Revealed by Interferometric Scattering Microscopy ELife. Mar, 2015 | Pubmed ID: 25748137 Four Things to Know About Myosin Light Chains As Reporters for Non-muscle Myosin-2 Dynamics in Live Cells Cytoskeleton (Hoboken, N.J.). Feb, 2015 | Pubmed ID: 25712372 Kinetic Characterization of the Sole Nonmuscle Myosin-2 from the Model Organism Drosophila Melanogaster FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology. Apr, 2015 | Pubmed ID: 25636739 Myosin Light Chains: Teaching Old Dogs New Tricks Bioarchitecture. 2014 | Pubmed ID: 26155737 Chaperone-enhanced Purification of Unconventional Myosin 15, a Molecular Motor Specialized for Stereocilia Protein Trafficking Proceedings of the National Academy of Sciences of the United States of America. Aug, 2014 | Pubmed ID: 25114250 Use of Fluorescent Techniques to Study the in Vitro Movement of Myosins Experientia Supplementum (2012). 2014 | Pubmed ID: 25095996 Regulation of Nonmuscle Myosin II by Tropomyosin Biochemistry. Jun, 2014 | Pubmed ID: 24873380 Myosin-10 Produces Its Power-stroke in Two Phases and Moves Processively Along a Single Actin Filament Under Low Load Proceedings of the National Academy of Sciences of the United States of America. May, 2014 | Pubmed ID: 24753602 To Understand Muscle You Must Take It Apart Frontiers in Physiology. 2014 | Pubmed ID: 24653704 Characterization of Three Full-length Human Nonmuscle Myosin II Paralogs The Journal of Biological Chemistry. Nov, 2013 | Pubmed ID: 24072716 Actin Structure-dependent Stepping of Myosin 5a and 10 During Processive Movement PloS One. 2013 | Pubmed ID: 24069366 Kinetic Characterization of the ATPase and Actin-activated ATPase Activities of Acanthamoeba Castellanii Myosin-2 The Journal of Biological Chemistry. Sep, 2013 | Pubmed ID: 23897814 Loss of Actomyosin Regulation in Distal Arthrogryposis Myopathy Due to Mutant Myosin Binding Protein-C Slow FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology. Aug, 2013 | Pubmed ID: 23657818 Myosin Regulatory Light Chain (RLC) Phosphorylation Change As a Modulator of Cardiac Muscle Contraction in Disease The Journal of Biological Chemistry. May, 2013 | Pubmed ID: 23530050 Mammalian Myosin-18A, a Highly Divergent Myosin The Journal of Biological Chemistry. Mar, 2013 | Pubmed ID: 23382379 Kinetic Characterization of Nonmuscle Myosin IIb at the Single Molecule Level The Journal of Biological Chemistry. Jan, 2013 | Pubmed ID: 23148220 Dynamic Exchange of Myosin VI on Endocytic Structures The Journal of Biological Chemistry. Nov, 2012 | Pubmed ID: 22992744 Nonmuscle Myosin II Exerts Tension but Does Not Translocate Actin in Vertebrate Cytokinesis Proceedings of the National Academy of Sciences of the United States of America. Mar, 2012 | Pubmed ID: 22393000 Walking to Work: Roles for Class V Myosins As Cargo Transporters Nature Reviews. Molecular Cell Biology. Dec, 2011 | Pubmed ID: 22146746 Regulation of Myosin 5a and Myosin 7a Biochemical Society Transactions. Oct, 2011 | Pubmed ID: 21936778 Myosin Motor Proteins Are Involved in the Final Stages of the Secretory Pathways Biochemical Society Transactions. Oct, 2011 | Pubmed ID: 21936774 Nucleotide Pyrophosphatase Employs a P-loop-like Motif to Enhance Catalytic Power and NDP/NTP Discrimination Proceedings of the National Academy of Sciences of the United States of America. Aug, 2011 | Pubmed ID: 21831832 Dynein Struts Its Stuff Nature Structural & Molecular Biology. Jun, 2011 | Pubmed ID: 21642959 Light-triggered Myosin Activation for Probing Dynamic Cellular Processes Angewandte Chemie (International Ed. in English). Jun, 2011 | Pubmed ID: 21542072 Drosophila Melanogaster Myosin-18 Represents a Highly Divergent Motor with Actin Tethering Properties The Journal of Biological Chemistry. Jun, 2011 | Pubmed ID: 21498886 The Kinetic Mechanism of Mouse Myosin VIIA The Journal of Biological Chemistry. Mar, 2011 | Pubmed ID: 21212272 Myosin VI and Its Binding Partner Optineurin Are Involved in Secretory Vesicle Fusion at the Plasma Membrane Molecular Biology of the Cell. Jan, 2011 | Pubmed ID: 21148290 Nonmuscle Myosin IIA with a GFP Fused to the N-terminus of the Regulatory Light Chain is Regulated Normally Journal of Muscle Research and Cell Motility. Sep, 2010 | Pubmed ID: 20711642 Direct Observation of the Myosin-Va Power Stroke and Its Reversal Nature Structural & Molecular Biology. May, 2010 | Pubmed ID: 20418880 Influence of Lever Structure on Myosin 5a Walking Proceedings of the National Academy of Sciences of the United States of America. Feb, 2010 | Pubmed ID: 20133809 Extensibility of the Extended Tail Domain of Processive and Nonprocessive Myosin V Molecules Biophysical Journal. Dec, 2009 | Pubmed ID: 20006949 A FERM Domain Autoregulates Drosophila Myosin 7a Activity Proceedings of the National Academy of Sciences of the United States of America. Mar, 2009 | Pubmed ID: 19255446 An Alternatively Spliced Isoform of Non-muscle Myosin II-C is Not Regulated by Myosin Light Chain Phosphorylation The Journal of Biological Chemistry. Apr, 2009 | Pubmed ID: 19240025 Switch 1 Mutation S217A Converts Myosin V into a Low Duty Ratio Motor The Journal of Biological Chemistry. Jan, 2009 | Pubmed ID: 19008235 The Small Molecule Tool (S)-(-)-blebbistatin: Novel Insights of Relevance to Myosin Inhibitor Design Organic & Biomolecular Chemistry. Jun, 2008 | Pubmed ID: 18528569 Mutation Spectrum of MYO7A and Evaluation of a Novel Nonsyndromic Deafness DFNB2 Allele with Residual Function Human Mutation. Apr, 2008 | Pubmed ID: 18181211 Calcium and Cargoes As Regulators of Myosin 5a Activity Biochemical and Biophysical Research Communications. Apr, 2008 | Pubmed ID: 18060865 The B2 Alternatively Spliced Isoform of Nonmuscle Myosin II-B Lacks Actin-activated MgATPase Activity and in Vitro Motility Biochemical and Biophysical Research Communications. Apr, 2008 | Pubmed ID: 18060863 Folding and Regulation in Myosins II and V Journal of Muscle Research and Cell Motility. 2007 | Pubmed ID: 18427938 Myo4p is a Monomeric Myosin with Motility Uniquely Adapted to Transport MRNA The Journal of Cell Biology. Sep, 2007 | Pubmed ID: 17893244 Diversity of Structural Behavior in Vertebrate Conventional Myosins Complexed with Actin Journal of Molecular Biology. May, 2007 | Pubmed ID: 17433365 Loop 2 of Limulus Myosin III is Phosphorylated by Protein Kinase A and Autophosphorylation Biochemistry. Apr, 2007 | Pubmed ID: 17367164 In Vitro Reconstitution of a Transport Complex Containing Rab27a, Melanophilin and Myosin Va FEBS Letters. Oct, 2006 | Pubmed ID: 17045265 The Cargo-binding Domain Regulates Structure and Activity of Myosin 5 Nature. Jul, 2006 | Pubmed ID: 16838021 Dimerized Drosophila Myosin VIIa: a Processive Motor Proceedings of the National Academy of Sciences of the United States of America. Apr, 2006 | Pubmed ID: 16585515 Walking with Myosin V Current Opinion in Cell Biology. Feb, 2006 | Pubmed ID: 16378722 Step-size is Determined by Neck Length in Myosin V Biochemistry. Dec, 2005 | Pubmed ID: 16331980 Myosin VIIB from Drosophila is a High Duty Ratio Motor The Journal of Biological Chemistry. Sep, 2005 | Pubmed ID: 16055438 Myosin V from Drosophila Reveals Diversity of Motor Mechanisms Within the Myosin V Family The Journal of Biological Chemistry. Aug, 2005 | Pubmed ID: 15980429 Disease-associated Mutations and Alternative Splicing Alter the Enzymatic and Motile Activity of Nonmuscle Myosins II-B and II-C The Journal of Biological Chemistry. Jun, 2005 | Pubmed ID: 15845534 Mechanism of Action of Myosin X, a Membrane-associated Molecular Motor The Journal of Biological Chemistry. Apr, 2005 | Pubmed ID: 15705568 Myosin-XVa is Required for Tip Localization of Whirlin and Differential Elongation of Hair-cell Stereocilia Nature Cell Biology. Feb, 2005 | Pubmed ID: 15654330 Blebbistatin, a Myosin II Inhibitor, is Photoinactivated by Blue Light Biochemistry. Jan, 2005 | Pubmed ID: 15641783 Fifty Years of Contractility Research Post Sliding Filament Hypothesis Journal of Muscle Research and Cell Motility. 2004 | Pubmed ID: 15630612 Specificity of Blebbistatin, an Inhibitor of Myosin II Journal of Muscle Research and Cell Motility. 2004 | Pubmed ID: 15548862 Nanometer Localization of Single Green Fluorescent Proteins: Evidence That Myosin V Walks Hand-over-hand Via Telemark Configuration Biophysical Journal. Sep, 2004 | Pubmed ID: 15345556 Mechanism of Blebbistatin Inhibition of Myosin II The Journal of Biological Chemistry. Aug, 2004 | Pubmed ID: 15205456 Two-headed Binding of the Unphosphorylated Nonmuscle Heavy Meromyosin.ADP Complex to Actin Biochemistry. Apr, 2004 | Pubmed ID: 15065866 Regulated Conformation of Myosin V The Journal of Biological Chemistry. Jan, 2004 | Pubmed ID: 14634000 Trifluoperazine Inhibits the MgATPase Activity and in Vitro Motility of Conventional and Unconventional Myosins Journal of Muscle Research and Cell Motility. 2003 | Pubmed ID: 14870973 Functional Divergence of Human Cytoplasmic Myosin II: Kinetic Characterization of the Non-muscle IIA Isoform The Journal of Biological Chemistry. Oct, 2003 | Pubmed ID: 12847096 Utility of Genetic Screening in Hypertrophic Cardiomyopathy: Prevalence and Significance of Novel and Double (homozygous and Heterozygous) Beta-myosin Mutations Genetic Testing. 2003 | Pubmed ID: 12820698 Neck Length and Processivity of Myosin V The Journal of Biological Chemistry. Aug, 2003 | Pubmed ID: 12740393 Kinetic Mechanism of Non-muscle Myosin IIB: Functional Adaptations for Tension Generation and Maintenance The Journal of Biological Chemistry. Jul, 2003 | Pubmed ID: 12704189 The Prepower Stroke Conformation of Myosin V The Journal of Cell Biology. Dec, 2002 | Pubmed ID: 12499355 Mutations in Human Nonmuscle Myosin IIA Found in Patients with May-Hegglin Anomaly and Fechtner Syndrome Result in Impaired Enzymatic Function The Journal of Biological Chemistry. Nov, 2002 | Pubmed ID: 12237319 Rab27a is an Essential Component of Melanosome Receptor for Myosin Va Molecular Biology of the Cell. May, 2002 | Pubmed ID: 12006666 Identification of an Organelle Receptor for Myosin-Va Nature Cell Biology. Apr, 2002 | Pubmed ID: 11887186 Myosin-I Nomenclature The Journal of Cell Biology. Nov, 2001 | Pubmed ID: 11724811 Analysis of the Regulatory Phosphorylation Site in Acanthamoeba Myosin IC by Using Site-directed Mutagenesis Proceedings of the National Academy of Sciences of the United States of America. Dec, 1998 | Pubmed ID: 9860946 A Myosin III from Limulus Eyes is a Clock-regulated Phosphoprotein The Journal of Neuroscience : the Official Journal of the Society for Neuroscience. Jun, 1998 | Pubmed ID: 9614231 Миозин-специфические адаптации анализов подвижности на основе флуоресцентной микроскопии in vitro Ananya Tripathi1, Charles Bond1,2, James R. Sellers1, Neil Billington1, Yasuharu Takagi1 1Laboratory of Molecular Physiology, Cell and Developmental Biology Center, National Heart, Lung and Blood Institute, National Institutes of Health, 2Department of Physiology, Perelman School of Medicine, University of Pennsylvania JoVE 62180 Biochemistry Полу-высокой пропускной адаптации NADH-связанных АТПас анализ для скрининга малых ингибиторы молекулы Laszlo Radnai1,2, Rebecca F. Stremel1,2, James R. Sellers3, Gavin Rumbaugh2, Courtney A. Miller1,2 1Department of Molecular Medicine, The Scripps Research Institute, 2Department of Neuroscience, The Scripps Research Institute, 3Laboratory of Molecular Physiology, NHLBI, National Institutes of Health JoVE 60017 Biochemistry
Миозин-специфические адаптации анализов подвижности на основе флуоресцентной микроскопии in vitro Ananya Tripathi1, Charles Bond1,2, James R. Sellers1, Neil Billington1, Yasuharu Takagi1 1Laboratory of Molecular Physiology, Cell and Developmental Biology Center, National Heart, Lung and Blood Institute, National Institutes of Health, 2Department of Physiology, Perelman School of Medicine, University of Pennsylvania JoVE 62180 Biochemistry
Полу-высокой пропускной адаптации NADH-связанных АТПас анализ для скрининга малых ингибиторы молекулы Laszlo Radnai1,2, Rebecca F. Stremel1,2, James R. Sellers3, Gavin Rumbaugh2, Courtney A. Miller1,2 1Department of Molecular Medicine, The Scripps Research Institute, 2Department of Neuroscience, The Scripps Research Institute, 3Laboratory of Molecular Physiology, NHLBI, National Institutes of Health JoVE 60017 Biochemistry
