In JoVE (1)
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Articles by Jeanne M. LeBon in JoVE
In Vitro Colony Assays for Characterizing Tri-potent Progenitor Cells Isolated from the Adult Murine Pancreas Jacob R. Tremblay*1,2, Jeanne M. LeBon*1, Angela Luo1, Janine C. Quijano1, Lena Wedeken1, Kevin Jou1, Arthur D. Riggs1, David A. Tirrell3, H. Teresa Ku1 1Diabetes and Metabolism Research Institute, Beckman Research Institute of City of Hope, 2Irell & Manella Graduate School of Biological Sciences, Beckman Research Institute of City of Hope, 3Division of Chemistry and Chemical Engineering, California Institute of Technology In vitro colony assays to detect self-renewal and differentiation of progenitor cells isolated from adult murine pancreas are devised. In these assays, pancreatic progenitors give rise to cell colonies in 3-dimensional space in methylcellulose-containing semi-solid medium. Protocols for handling single cells and characterization of individual colonies are described.