In JoVE (1)
Other Publications (1)
Articles by Jenessa A. Winston in JoVE
Cefoperazone-treated Mouse Model of Clinically-relevant Clostridium difficile Strain R20291 Jenessa A. Winston1, Rajani Thanissery1, Stephanie A. Montgomery2, Casey M. Theriot1 1Department of Population Health and Pathobiology, North Carolina State University College of Veterinary Medicine, 2Department of Pathology and Laboratory Medicine, Lineberger Comprehensive Cancer Center, University of North Carolina School of Medicine This protocol outlines the cefoperazone mouse model of Clostridium difficile infection (CDI) using a clinically-relevant and genetically-tractable strain, R20291. Emphasis on clinical disease monitoring, C. difficile bacterial enumeration, toxin cytotoxicity, and histopathological changes throughout CDI in a mouse model are detailed in the protocol.
Other articles by Jenessa A. Winston on PubMed
Impact of Microbial Derived Secondary Bile Acids on Colonization Resistance Against Clostridium Difficile in the Gastrointestinal Tract Anaerobe. Oct, 2016 | Pubmed ID: 27163871 Clostridium difficile is an anaerobic, Gram positive, spore-forming bacillus that is the leading cause of nosocomial gastroenteritis. Clostridium difficile infection (CDI) is associated with increasing morbidity and mortality, consequently posing an urgent threat to public health. Recurrence of CDI after successful treatment with antibiotics is high, thus necessitating discovery of novel therapeutics against this pathogen. Susceptibility to CDI is associated with alterations in the gut microbiota composition and bile acid metabolome, specifically a loss of microbial derived secondary bile acids. This review aims to summarize in vitro, ex vivo, and in vivo studies done by our group and others that demonstrate how secondary bile acids affect the different stages of the C. difficile life cycle. Understanding the dynamic interplay of C. difficile and microbial derived secondary bile acids within the gastrointestinal tract will shed light on how bile acids play a role in colonization resistance against C. difficile. Rational manipulation of secondary bile acids may prove beneficial as a treatment for patients with CDI.