In JoVE (1)
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Articles by Jenna M Guynn in JoVE
Procedure and Key Optimization Strategies for an Automated Capillary Electrophoretic-based Immunoassay Method Gail M Nelson1, Jenna M Guynn2, Brian N Chorley1 1National Health and Environmental Effects Research Laboratory, U.S. Environmental Protection Agency, 2Oak Ridge Institute for Science and Education at U.S. Environmental Protection Agency A capillary-based immunoassay using a commercial platform to measure target proteins from total protein preparations is demonstrated. In addition, assay parameters of exposure time, protein concentration, and antibody dilution are optimized for a cell culture model system.