Articles by Jeremy Hughes in JoVE
Renal Ischaemia Reperfusion Injury: A Mouse Model of Injury and Regeneration Emily E. Hesketh1, Alicja Czopek1, Michael Clay1, Gary Borthwick1, David Ferenbach1, David Kluth1, Jeremy Hughes1 1MRC Centre for Inflammation Research, University of Edinburgh The mouse model of renal ischaemia reperfusion injury described here comprises of a right nephrectomy that provides control tissue and clamping of the left renal pedicle to induce ischaemia that results in acute kidney injury. This model uses a midline laparotomy approach with all steps performed via one incision.
Mouse Kidney Transplantation: Models of Allograft Rejection George H. Tse*1, Emily E. Hesketh*1, Michael Clay1, Gary Borthwick1, Jeremy Hughes1, Lorna P. Marson1 1MRC Centre for Inflammation Research, The Queen's Medical Research Institute, The University of Edinburgh Here, we present a protocol to study the immunology of rejection. The surgical model presented reports a short operating time and a concise technique. Depending on the donor-recipient strain combination, the transplanted kidney may develop acute cellular rejection or chronic allograft damage, defined by interstitial fibrosis and tubular atrophy.
A Murine Model of Irreversible and Reversible Unilateral Ureteric Obstruction Emily E. Hesketh1, Madeleine A. Vernon1, Peng Ding1, Spike Clay1, Gary Borthwick1, Bryan Conway1, Jeremy Hughes1 1MRC Centre for Inflammation Research, University of Edinburgh The murine model of irreversible unilateral ureteric obstruction (UUO) is presented together with the model of reversible UUO in which the ureteric obstruction is relieved by anastomosis of the severed ureter into the bladder. These models enable the study of renal inflammation and scarring as well as tissue remodeling.