Other Publications (2)
Articles by Jesse Barnes in JoVE
चूहे में Sucrose तरस के ऊष्मायन का मूल्यांकन करने के लिए एक सामान्य विधि Jeffrey W. Grimm1, Jesse Barnes1, Kindsey North1, Stefan Collins1, Rachel Weber1 1Department of Psychology and Program in Behavioral Neuroscience, Western Washington University भोजन या दवा बनती cues के लिए जवाब संयम की अवधि के दौरान बढ़ जाती है और यह एक वृद्धि की डूबने व्यवहार करने के लिए संवेदनशीलता के लिए संबंधित हो सकती है. यहाँ चूहों कि आत्म प्रशासित sucrose में इस लालसा के ऊष्मायन "का मूल्यांकन करने के लिए हम एक प्रक्रिया विस्तार.
Other articles by Jesse Barnes on PubMed
Aggregation of Granulocyte-colony Stimulating Factor in Vitro Involves a Conformationally Altered Monomeric State Protein Science : a Publication of the Protein Society. Sep, 2005 | Pubmed ID: 16131655 Aggregation of partially folded intermediates populated during protein folding processes has been described for many proteins. Likewise, partially unfolded chains, generated by perturbation of numerous proteins by heat or chemical denaturants, have also been shown to aggregate readily. However, the process of protein aggregation from native-state conditions is less well understood. Granulocyte-colony stimulating factor (G-CSF), a member of the four-helix bundle class of cytokines, is a therapeutically relevant protein involved in stimulating the growth and maturation of phagocytotic white blood cells. Under native-like conditions (37 degrees C [pH 7.0]), G-CSF shows a significant propensity to aggregate. Our data suggest that under these conditions, native G-CSF exists in equilibrium with an altered conformation, which is highly aggregation prone. This species is enriched in 1-2 M GdmCl, as determined by tryptophan fluorescence and increased aggregation kinetics. In particular, specific changes in Trp58 fluorescence report a local rearrangement in the large loop region between helices A and B. However, circular dichroism, reactivity toward cyanylation, and ANS binding demonstrate that this conformational change is subtle, having no substantial disruption of secondary and tertiary structure, reactivity of the free sulfhydryl at Cys17 or exposure of buried hydrophobic regions. There is no indication that this altered conformation is important to biological activity, making it an attractive target for rational protein stabilization.
Effects of Systemic or Nucleus Accumbens-directed Dopamine D1 Receptor Antagonism on Sucrose Seeking in Rats Psychopharmacology. Jul, 2011 | Pubmed ID: 21318562 Conditioned cues can elicit relapse to drug- and food-seeking behavior over prolonged periods of abstinence. If seeking behavior depends on mesolimbic dopamine D1 receptors, blocking these receptors should reduce seeking behavior.