In JoVE (1)
Other Publications (1)
Articles by Jessica M. Motherwell in JoVE
Other articles by Jessica M. Motherwell on PubMed
Re-endothelialization of Rat Lung Scaffolds Through Passive, Gravity-driven Seeding of Segment-specific Pulmonary Endothelial Cells Journal of Tissue Engineering and Regenerative Medicine. Dec, 2016 | Pubmed ID: 27943597 Effective re-endothelialization is critical for the use of decellularized scaffolds for ex vivo lung engineering. Current approaches yield insufficiently re-endothelialized scaffolds that hemorrhage and become thrombogenic upon implantation. Herein, gravity-driven seeding coupled with bioreactor culture facilitated widespread distribution and engraftment of endothelial cells throughout rat lung scaffolds. Initially, human umbilical vein endothelial cells (HUVECs) were seeded into the pulmonary artery by either gravity-driven, variable flow perfusion seeding or pump-driven, pulsatile flow perfusion seeding. Gravity seeding evenly distributed cells and supported cell survival and re-lining of the vascular walls while perfusion pump-driven seeding led to increased cell fragmentation and death. Using gravity seeding, rat pulmonary artery endothelial cells (PAECs) and rat pulmonary vein endothelial cells (PVECs) attached in intermediate and large vessels, while rat pulmonary microvascular endothelial cells (MVECs) deposited mostly in microvessels. Combination seeding of PAECs, PVECs, and MVECs led to positive VE-cadherin staining. In addition, combination seeding improved barrier function as assessed by serum albumin extravasation; however, leakage was observed in the distal portions of the re-endothelialized tissue suggesting that recellularization of the alveoli is necessary to complete barrier function of the capillary-alveolar network. Overall, these data indicate that vascular recellularization of rat lung scaffolds is achieved through gravity seeding. This article is protected by copyright. All rights reserved.